PURPOSE: To study the ultrastructural of rat intestinal villi (ileum) after total hepatic pedicle clumping for different period of time. METHODS: Forty male rats were divided into four groups of ten animals each. The Sham group was not being submitted to ischemia (control). E1, E2 and E3 group were submitted to 10, 20 and 30 minutes of ischemia, respectively. At the end of the experiment fragments of the ileum were taken off and examined by electromicroscope. RESULTS: Leucocyte infiltration, vascular dilation and electrotransparent areas were observed in intestinal villi of lamina propria of E1 and E2 groups. In reference to group E3 desquamation of epithelial layer, desquamation of intestinal villi and signs of apoptosis were observed. Hemorrhagic areas, dilated lymphatics and blood vessels and lots of leucocytes were observed in lamina propria. CONCLUSION: The clumping of hepatic pedicle provokes esplacnic congestion, time dependent, and to the 30 minutes were epithelial cell desquamation, apoptosis signs and hemorrhage.
Ischemia; Intestine; Rats; Ultrastructure
