Lycopene and resveratrol pretreatment did not interfere with the liver of hepatectomized rats1

The lycopene group and the resveratrol group received 40 mg/kg/day of lycopene or resveratrol, respectively (dissolved in olive oil or in saline solution, respectively) and administered via a gastric tube for 30 days. The partially hepatectomzed (PH) control groups received saline or olive oil via a gastric tube for 30 days, respectively, and the normal control group received no treatment. Liver tissue and intracardiac blood samples were obtained 24, 36 or 48 h after PH.

Results:

No areas of fibrosis were detected. No significant changes in mitotic index, in the number of apoptosis events or in aspartate aminotransferase and alanine aminotransferase levels were observed.

Conclusions:

Lycopene and resveratrol pretreatment did not interfere on hepatic hyperplasia in partially hepatectomized rats.

Key words :
Alanine Transaminase; Aspartate Aminotransferases; Apoptosis; Mitotic Index; Rats.

Authorship

Pedro Paulo Barros

PhD, Full Professor, Researcher, Faculty of Pharmaceutical Sciences, Pontifícia Universidade Católica de Campinas (PUC-Campinas), Brazil. Conception of the study, interpretation of data.Pontifícia Universidade Católica de CampinasBrazilBrazilPhD, Full Professor, Researcher, Faculty of Pharmaceutical Sciences, Pontifícia Universidade Católica de Campinas (PUC-Campinas), Brazil. Conception of the study, interpretation of data.

Gisele Mara Silva Gonçalves

PhD, Full Professor, Researcher, Faculty of Pharmaceutical Sciences, PUC-Campinas, Brazil. Conception of the study, interpretation of data. PUCBrazilCampinas, BrazilPhD, Full Professor, Researcher, Faculty of Pharmaceutical Sciences, PUC-Campinas, Brazil. Conception of the study, interpretation of data.

Gustavo Henrique da Silva

Maria Clara Villaça Dias Bastos

Graduate student, School of Medicine, FAPIC, PUC-Campinas, Brazil. Interpretation of data.PUCBrazilCampinas, BrazilGraduate student, School of Medicine, FAPIC, PUC-Campinas, Brazil. Interpretation of data.

Loren Nogaroto Ramos

Marilia Marinello Fernandes

Correspondence: Pedro Paulo Barros Faculdade de Ciências Farmacêuticas Avenida John Boyd Dunlop, s/n 13060-904 Campinas - SP Brasil Tel.: (55 19)3343-6865 barrospp@puc-campinas.edu.br

Conflict of interest:

none

SCIMAGO INSTITUTIONS RANKINGS

Figures | Tables

Figures (4)
Tables (2)

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Figure 1
Experimental design.

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Figure 2
Liver, Hepatocyte - Mitosis (asterisks) and Apoptosis (arrows) in Wistar rats. Control groups after 36 and 48 hours of partial hepatectomy (PH1 36, PH1 48, PH2 36 and PH 48, respectively). Lycopene or resveratrol groups, after 36 and 48 hours of partial hepatectomy (LPH 36, LPH 48, RPH 36 and RPH 48, respectively. Feulgen stain, x400.

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Figure 3
Clinical parameters of rats submitted to Partial Hepatectomy, and Lycopene + Partial Hepatectomy. AST=Aspartate aminotransferase; ALT=Alanine aminotransferase. PH24, PH36 and PH48: Groups examined 24, 36 and 48 h after partial hepatectomy, respectively; LPH24, LPH36 and LPH48: Lycopene-treated groups examined at 24, 36 and 48 h, respectively. The Kruskal-Wallis test and Dunn's post-test showed no significant difference when each group was compared to its respective control. AST =15.4.± 4.71 and ALT =58.72± 27.2 for the Normal Control Group

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Figure 4
Clinical parameters of rats submitted to Partial Hepatectomy, and Resveratrol + Partial Hepatectomy rats. AST=Aspartate aminotransferase; ALT=Alanine aminotransferase. PH24, PH36 and PH48: Partial hepatectomy groups 24, 36 and 48 hours, respectively; RPH24, RPH36 and RPH48: Resveratrol treated groupS 24, 36 and 48 hours, respectively. The Kruskal-Wallis test and Dunn's post-test showed no significant difference when each group was compared to its respective control. AST =15.4.± 4.71 and ALT =58.72± 27.2 for the Normal Control Group.

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Table 1
Mitotic index and apoptosis index for Partial Hepatectomy (PH2) and Resveratrol+Partial Hepatectomy hepatic tissues (RPH).

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Table 2
Mitotic index and apoptosis index for Partial Hepatectomy (PH1), and Lycopene + Partial Hepatectomy hepatic tissues (LPH).

Figure 1 Experimental design.

Figure 2 Liver, Hepatocyte - Mitosis (asterisks) and Apoptosis (arrows) in Wistar rats. Control groups after 36 and 48 hours of partial hepatectomy (PH1 36, PH1 48, PH2 36 and PH 48, respectively). Lycopene or resveratrol groups, after 36 and 48 hours of partial hepatectomy (LPH 36, LPH 48, RPH 36 and RPH 48, respectively. Feulgen stain, x400.

Figure 3 Clinical parameters of rats submitted to Partial Hepatectomy, and Lycopene + Partial Hepatectomy. AST=Aspartate aminotransferase; ALT=Alanine aminotransferase. PH24, PH36 and PH48: Groups examined 24, 36 and 48 h after partial hepatectomy, respectively; LPH24, LPH36 and LPH48: Lycopene-treated groups examined at 24, 36 and 48 h, respectively. The Kruskal-Wallis test and Dunn's post-test showed no significant difference when each group was compared to its respective control. AST =15.4.± 4.71 and ALT =58.72± 27.2 for the Normal Control Group

Figure 4 Clinical parameters of rats submitted to Partial Hepatectomy, and Resveratrol + Partial Hepatectomy rats. AST=Aspartate aminotransferase; ALT=Alanine aminotransferase. PH24, PH36 and PH48: Partial hepatectomy groups 24, 36 and 48 hours, respectively; RPH24, RPH36 and RPH48: Resveratrol treated groupS 24, 36 and 48 hours, respectively. The Kruskal-Wallis test and Dunn's post-test showed no significant difference when each group was compared to its respective control. AST =15.4.± 4.71 and ALT =58.72± 27.2 for the Normal Control Group.

Table 1 Mitotic index and apoptosis index for Partial Hepatectomy (PH2) and Resveratrol+Partial Hepatectomy hepatic tissues (RPH).

Mitotic index
PH24	RPH24	PH36	RPH36	PH48	RPH48
39.44±7.4	26.78±7.1	30.25±4.6	19.82±2.9	21.85±3.4	15.79±5.0
Apoptotic index				21.85±3.4	15.79±5.0
PH24	RPH24	PH36	RPH36	PH48	RPH48
0.36±0.3	1.00 ±1.0	2.23±3.3	4.62±4.8	9.62±8.3	10.78±10.3

PH24, PH36 and PH48: Groups examined 24, 36 and 48 h after partial hepatectomy, respectively; RPH24, RPH36 and RPH48: Resveratrol-treated groups examined 24, 36 and 48h after partial hepatetomy, respectively. The Kruskal-Wallis Test and Dunn's post-test showed no significant difference when each group was compared to its respective control.

Table 2 Mitotic index and apoptosis index for Partial Hepatectomy (PH1), and Lycopene + Partial Hepatectomy hepatic tissues (LPH).

Mitotic Index
PH24	LPH24	PH36	LPH36	PH48	LPH48
14.67±9.7	23.45±5.5	7.34±1.3	12.44±6.4	10.46±2.0	9.22±3.5
Apoptosis Index				10.46±2.0	9.22±3.5
PH24	LPH24	PH36	LPH36	PH48	LPH48
0.49±0.4	0.36±0.7	1.01±0.9	1.80±1.4	5.93±5.1	8.64±6.4

PH24, PH36 and PH48: Groups examined 24, 36 and 48 h after partial hepatectomy, respectively; LPH24, LPH36 and LPH48: Lycopene-treated groups examined 24, 36 and 48h after partial hepatetomy, respectively. The Kruskal-Wallis Test and Dunn's post-test showed no significant difference when each group was compared to its respective control.

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