The ileum positively regulates hepatic regeneration in rats 1

PURPOSE: To test the hypothesis that liver regeneration after partial hepatectomy can be influenced by the ileum. METHODS: Eighteen Wistar rats were distributed into groups of six animals: 1 ileum resection+ hepatectomy 2/3; 2 hepatectomy 2/3, and 3 sham. Anesthesia with ketamine and xylazine i.p., aseptic technique, analgesia with meperidine (10mg/kg s.c.). On day 6, serum ALT, AST, alkaline phosphatase (AP) and albumin were measured. Liver regeneration and hepatocyte mitosis were quantified. Statistical analysis with ANOVA and Tukey tests, with significance p<0.05. RESULTS: In group hepatectomy+ileal resection, ALT, AST and AP were 180.6±24.9, 58.6±3.1 and 254.6±46.6 respectively. They were significantly higher than in the hepatectomy group, whose values were 126.0±16.5, 44.1±3.9 and 163.5±8.6, respectively (p<0.001). Albumin levels were not significantly different among groups. Liver regeneration in hepatectomy group (94.17%) was statistically higher (p<0.001) than in ileal resection+hepatectomy group (55.96%). In the latter group the mitosis of hepatocytes were significantly less frequent than in the hepatectomy group. CONCLUSION: The data confirm that the ileum positively influence on liver regeneration in rats undergoing hepatectomy.


Introduction
Some analyzes have been conducted to investigate factors that regulate liver mass and regeneration like hormones 1,2 , and other factors 3 , but the nature of the signals have not been fully elucidated.Some mechanisms derived from intestine and portal circulation contribute to the regulation of the liver mass/body mass index.The role of the small intestine in regulating the hepatic regeneration after injury has been investigated, suggesting that intestine is the source of some humoral factors for regeneration after partial hepatectomy [4][5][6][7] .Some mechanisms derived from intestine and portal circulation may contribute to the regulation of the relative liver/body mass 4,5 .Other studies indicate that adaptation of small intestine post-resection, as well as ischemia/ reperfusion may influence regenerative liver response 8,9 .Huang et al. 10 demonstrated the factor named FXR which affects the absorption of bile acids which may regulate the liver mass and its recovery after injuries, supporting the role of the small intestine in the regulation of regeneration.Much is known about the signals and mechanisms of regeneration, both derived from the ligant/ receiver system, and from the whole body, including circulatory events and signals from other organs.Hepatocellular carcinoma study suggested that there may be more mechanisms contributing to the regulation of hepatocyte growth than those currently known 11 .
Liver regeneration after partial 2/3 hepatectomy in rats has been shown to be a useful model for the study of liver regeneration.
Based on these data, this study aims to test the hypothesis that liver regeneration after partial hepatectomy can be specifically regulated by ileum, intestinal segment that may be effective in the process.An experimental model of ileal resection examined the liver mass/body weight ratio, liver function tests and parameters of liver regeneration.

Methods
The experimental procedures performed in the study were done in accordance to the law nº 11.974/08, Brazil, and approved by the Institutional Animal Care and Ethics Committee.
Eighteen Wistar rats were housed in individual polypropylene cages with cycles of 12 h light-dark, with ad libitum access to water and food (Labina-Purina ® ), previously passing through a period of seven days acclimation in the laboratory.
One day before the operations, the animals were kept on liquid diet.Rats were distributed into 3 groups, each with 6 rats: group 1, hepatectomy+ ileonectomy; group 2, hepatectomy; group 3, sham.The rats were anesthetized with intraperitoneal (i.p.) injection of ketamine (50 mg/kg) and xylazine (20 mg/kg), and operated under aseptic technique.The postoperative pain was controlled with meperidine 10 mg / kg s.c., once every 12 hours.
The ileum resection (30 cm long) was made from 2 cm proximal to the caecum.The intestinal continuity was restored by end-to-end anastomosis in a single running suture, using 6-0 polypropylene with a surgical microscope DFV (Sao Paulo, Brazil), up to 10x magnification.The sham operation consisted of laparotomy and manipulation of the ileum with atraumatic forceps; the abdominal wound was then sutured.The partial 2/3 hepatectomy consisted of resection of the median and left lobes.
After the operations, animals received only water for the first 24 hours, followed by solid diet until euthanasia.The animals were weighed before surgery and every day until the 6 th observation day.

Biochemical measures
Serum from whole blood collected by cardiac puncture on the 6 th postoperative day was obtained by centrifugation at 1500 rpm for 10 minutes and stored at -30ºC until dosages.Serum ALT, AST, alkaline phosphatase and albumin were measured in all animals.

Calculation of liver regeneration
Firstly we calculated the liver mass/body mass ratio (LMBMR).After the observation period the rats were weighed (B), the liver completely removed and weighed on a precision balance (A).The data obtained were expressed as the percentage ratio of A to B multiplied by 100.The LMBMR was calculated by the formula: Liver mass LMBMR= __________ x 100

Body mass
The preoperative liver total weight was calculated using the average LMBMR of sham group at the time of euthanasia.
Changes in LMBMR on animals of groups 1, 2 and 3 were considered as degree of liver regeneration.The liver regeneration (LR) was calculated by the formula: LMBMReuthanasia -LMBMRpostop %LR= __________________________________x 100

LMBMRpostop
The ileum positively regulates hepatic regeneration in rats Acta Cirúrgica Brasileira -Vol.29 (2) 2014 -95 Histology Euthanasia of animals was done by an overdose of anesthetic (thiopental 100 mg/kg i.p.).A sample of the right hepatic lobe was fixed in buffered formalin 10%.Otherwise, tissue fractions were processed to be assessed for microscopic examination.Tissue slices were placed in cassettes and embedded in paraffin wax blocks.The blocks were then cut into 4 µm sections using a microtome, mounted onto slides, dried and stained with haematoxylin and eosin.Slides were then viewed in a standard manner.Liver histology was performed to quantify the number of cells undergoing mitosis using BX50 microscope (Olympus, Tokyo, Japan).For each animal, five blades were made, and 10 microscopic fields were examined in each slide (x400 magnification).Quantitation was obtained using video-assisted software (Image ProPlus 6.0, Cyber Media).

Statistical analysis
The data were analyzed using SPSS 18.0 (Chicago, IL, USA).To verify that the results obtained had statistically significant differences the following parametric tests were used: 1) Student's t -independent samples to compare between two groups, 2) analysis of variance (ANOVA) -to compare more than two groups, 3) Tukey test -used when the ANOVA showed significance, 4) Non-parametric Kruskal Wallis test -to check for differences between the three groups, when there was no homogeneity of variances, and 5) Dunnett test -when the Kruskal Wallis test showed significance; this test was used to determine in which groups the differences were statistically significant.For all tests the differences were significant when p<0.05.

Results
All animals survived until the end of the observation period and showed no postoperative complications.The animals in groups hepatectomy+ileonectomy and hepatectomy showed progressive reduction in weight until the 6 th postoperative day, which was not observed in the sham group.In the hepatectomy+ileonectomy group the ALT, AST and AP were 180.6±24.9,58.6±3.1 and 254.6±46.6 respectively, significantly higher than in the hepatectomy group values 126.0±16.5, 44.1±3.9 and 163.5±8.6,respectively (p<0.001).The albumin showed lower levels in the group undergoing hepatectomy+ileonectomy when compared with the other groups, but the difference was not significant (p=0.07).These data are summarized in Table 1.
Liver regeneration of sham group rats was calculated.
However, it showed an average 0.0.This was due to the fact that hepatic regeneration calculations were made considering the measures of the livers of animals from this group.
As we calculated liver mass/body mass ratio (LMBMR), using the livers of the sham group rats, it was possible to find a LMBMR average of 0.0318.So, the weight of the liver of rats -in the same conditions as age, sex and race -represented 3.18% of their body weight.Histological analysis revealed that in liver of animals undergoing hepatectomy+ileonectomy occurred a number of hepatocyte mitosis (75) significantly lower than in animals undergoing hepatectomy alone (154) as shown in Figure 1. Figure 2 shows liver cells undergoing mitosis in the blades B and B1 from hepatectomy group rats.The experimental model of this work has been adequate

Data in
and widely used, based on two important aspects.Firstly, the removal of resected liver tissue is not associated with severe hepatic necrosis.Second, due to the multilobular structure of rat liver, hepatectomy is "clean" and easy.Thus, the regeneration of residual lobes is soon mediated by processes in liver tissue.
Necrosis or severe inflammation there has not been observed 12 .
It was previously demonstrated increased intestinal expression of transforming growth factor after intestinal resection in rodents 13 .Hepatic growth factor has been shown in the intestine remaining after ileal resection in rats 14 .Concerning to IL-6, its serum levels were shown increased after 70% ileum resection in rats, but without statistical significance 15 .These data reinforce the argument against the fact that resection of the ileum induces deficiency of these factors to slow liver regeneration.
Another factor that may have contributed to the attenuation of liver regeneration in our study, after bowel resection, was the fact that the lack of the ileum implies lower supply of nutrients to the liver after extensive bowel resection.This nutritional problem has resulted in the reduction of gluconeogenic precursors from the intestine to the liver 16 .
It has been shown that rats initially lose weight, as occurred in the animals of this study, but become anabolic by the third postoperative day 17 .Was observed in our animals a reduction in the levels of albumin in the group undergoing hepatectomy+ileonectomy, with no significant difference compared with the other groups.Thus, we do not believe that nutritional changes could occur in our rats.They were initially normal and were observed for only six days.Assuming that serum albumin levels were not affected, nutrition could not interfere on our findings of liver regeneration.

Discussion
In the present study we worked with the hypothesis that resection of the distal small bowel could interfere with liver regeneration after hepatectomy.Our data demonstrated that resection of ileum impaired liver regeneration after 70% The ileum positively regulates hepatic regeneration in rats Acta Cirúrgica Brasileira -Vol.29 (2) 2014 -97 In several animal models it has been demonstrated that bacterial translocation to the mesenteric lymph nodes, liver and spleen is increased after intestinal resection [18][19][20] .Although in this study we have not studied bacterial translocation, it is speculated that the mechanism for this to occur is not yet known, but hardly is secondary to simple changes in intestinal permeability after ileal resection 21 .It was also shown that bacterial translocation occurs after major hepatic resection 22 .Endotoxins were shown to have a significant negative effect on liver regeneration after partial hepatectomy 23 .Thus, it is possible that the increase of endotoxins and bacteria into the portal blood, which has been demonstrated after intestinal and liver resection, could have affected liver regeneration.
The experimental model combining intestinal and liver resection is not new.Kahn et al. 24 in 1990 reported an increase in liver regenerative response when a ileocolectomy was performed in pigs.The authors concluded that the proximal colon can be a source of regenerating liver inhibitory factor, unlike what was observed after ileum resection.Despite the colon resection was not performed in the present study, we intend to use this model in the near future in this research line.In other studies, impaired liver regeneration after bowel resection has been demonstrated [25][26][27][28] , confirming the findings of the present study.

Conclusion
The data obtained showed that the ileum resection negatively influences the liver regeneration in rats undergoing hepatectomy.

FIGURE 2 -
FIGURE 2 -Postoperative histological analysis.Liver sections from Wistar rats were stained with HE for the presence of mitosis.A and A1: microphotographs from hepatectomy + ileal resection rats demonstrated the presence of normal hepatocytes and rare mitosis.B and B1: representative microphotographs from day 6 after hepatectomy rats showed several mitosis (arrows); magnification x400.