Abstract in English:PURPOSE: To investigate if the methanolic extract of the Otostegia persica can accelerating healing process of burn wound because of its anti-inflammatory and antioxidant effects. METHODS:Forty eight male Wistar rats were randomized into three study groups of 16 rats each. Burn wounds were created on dorsal part of shaved rats using a metal rod. In group I the burn wound was left without any treatment. Group was treated with topical silver sulfadiazine pomade. In group III, ointment containing the OP extract was administered. Skin biopsies were harvested from burn area on the 3rd, 5th, 14th and 21st days after burn and examined histologically. RESULTS: Re-epithelialization in the control group and in group II was lower than in group III. Re-epithelialization in groups II and III was significantly different from that in the control group. On the 5th day of the experiment, we assessed lower inflammation in the burn area compared to control group. This means that the inflammation was suppressed by methanolic extract of OP. From day 5 to 14; the fibroblast proliferation peaked and was associated with increased collagen accumulation. It was obvious that angiogenesis improved more in the groups II and III, which facilitated re-epithelialisation. CONCLUSION:Methanolic extract of Otostegia persica exhibited significant healing activity when topically applied on rats. OP is an effective treatment for saving the burn site.
Abstract in English:PURPOSE: To evaluate the bone repair process in ovariohysterectomized rabbit submitted to an ovarian transplant to stomach that may supplying some quantity of estrogen occurs to improve bone healing. METHODS: In 20 female rabbits three holes of 1, 2 and 3mm diameter in tibial shaft were made and after that all animals received OHE through a ventral incision and they were randomly divided into two groups of ten rabbits each. In group one, animals received one of their self-ovaries that transplanted on serosal layer of stomach and group two did not receive treatment. Animals were kept during bone healing for a period of 45 days and radiological, biochemical, biomechanical and histopathological evaluation. RESULTS: The tibial defects in group one healed completely after 45 days and had more callous than second group. There is significant difference between two groups after operation in 21, 28 and 35 days about estrogen, progesterone and phosphatase Alkaline. The maximum forces in group one, were significantly higher than that for the group two. CONCLUSION:Ovarian transplantation prevents the effects of ovariohysterectomized on bone healing of rabbit tibia, suggesting that unilateral transplanted ovaries can substitute for the action of ovaries on the skeleton in ovariohysterectomized animals.
Abstract in English:PURPOSE:To investigate the seminiferous tubule histological morphology after an 8 mmHg pneumoperitoneum in the rat model. METHODS: Fourteen rats were divided into two groups: a Sham group submitted to anesthesia and a pneumoperitoneum (Pp) group submitted to abdominal insufflation at 8 mmHg during three hours, followed by desuflation. All rats were killed after six weeks, testicles were collected and evaluated for the tubule diameter, germinative epithelium height and Johnsen´s score. Means were compared by using the Student's-t-test. RESULTS:The seminiferous tubule diameter was diminished by 11.3% in the group submitted to pneumoperitoneum (p<0.05). No significant difference was found among the groups when analyzing the epithelium height and Johnsen´s score. CONCLUSION:In the rat model, the seminiferous tubules present structural alterations when subjected to pneumoperitoneum of 8 mmHg during three hours.
Abstract in English:PURPOSE: To evaluate the effect of decompression fasciotomy on testes undergoing torsion of the spermatic cord. METHODS: Spermatic cord torsion was induced in 40 Wistar testes for six and 12 hours. At the time of distortion, decompression fasciotomy was performed on half of the animals. After 42 days, the testicles were examined both macroscopically and microscopically. A non-parametric Mann- Whitney U- test was used for statistical analysis. The results were considered significant at p≤0.05. RESULTS: Testicular weight; volume and degree of histological alterations were evaluated. When the groups without and with fasciotomy were compared, a 12-hour torsion subgroup in the fasciotomy group was noted to present larger volume, heavier weight and superior histological classification when compared with the 12-hour subgroup in the without fasciotomy group. No differences between the two groups after six hours of torsion were found. No significant changes were noticed in the contralateral testis. CONCLUSIONS: Testicular fasciotomy (decompression) benefited the macroscopic and histological aspects of the testicles undergoing cord torsion for a 12-hour period. These results demonstrate the role played by compartment syndrome in the pathophysiology of spermatic cord torsion and the need for decompression.
Abstract in English:PURPOSE: To evaluate the effects of andiroba oil on the periodontitis in rats. METHODS: The periodontitis was induced by the placement of cotton ligatures around the cervix of the second upper molars on fifteen rats, and waiting fifty days. The animals were randomly distributed into three groups: saline group, andiroba oil group and meloxican group, differentiated by substance used in the treatment of periodontitis. The groups received the respective substance by gavage for seven days, after the periodontitis induced. It was analyzed the score of inflammatory cells and the measurement from the cemento-enamel junction to the bone crest. RESULTS: The andiroba oil group (p=0.008) and meloxican group (p=0.0347) show a less score of inflammatory cells than saline group, however there weren't difference between them (p=0.2754). Regarding the analysis of measurement from the cemento-enamel junction to the bone crest, there was no difference between groups studied (p=0.3451). CONCLUSION: Andiroba oil decreased the quantity of inflammatory cells, however, it didn't have an effect on the measurement of alveolar bone loss, like the treatment with Meloxican®.
Abstract in English:PURPOSE: To evaluate the effect of bioflavonoid ternatin (TRT) on rat liver regeneration and oxidative stress after 70% partial hepatectomy (PH). METHODS: Thirty six young male Wistar rats were randomly assigned to two groups of 18 animals each - control (G1) and experimental (G2) - and were submitted to PH under inhalatory diethylether anesthesia. G1 rats received daily intraperitoneal (ip) injections of saline (NaCl 0.9% solution) 0.1 mL/kg for 14 days; G2 animals received daily ip injections of TRT 0.1% 1.0mg/kg for 14 days. At 36h (T1), 168h (T2) and 336h (T3) post-PH timepoints, a subgroup of six rats in each group was chosen in a randomized way to complementary hepatectomy (CH) and blood samples haversting. Collected material was saved for laboratory analysis (total bilirubin (TB), D-Glucose, glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) and assessment of liver regeneration. RESULTS: TRT induced a significant decrease in liver and plasma GSH concentrations; liver regeneration process was not affected. TRT promoted a significant decrease in blood glucose levels 168h after partial hepatectomy compared with controls. TB levels remained unchanged. CONCLUSION: Intraperitoneal bioflavonoid ternatin injection in partially hepatectomized rats induces a decrease in oxidative stress and a significant hypoglycemic state, but does not promote any change in the evolution of liver regeneration.
Abstract in English:PURPOSE: To investigate the protective effects of ischemic pre and postconditioning, as well as the association of both methods, in skeletal muscle injury produced by ischemia and reperfusion in rats. METHODS: An experimental study was designed using 40 Wistar rats divided in four groups (n=10): Control - rats submitted to ischemia for 240 minutes (min) and reperfusion for 60 min; Ischemic preconditioning (Pre) - animals submitted to three cycles of clamping and releasing the aorta for five min before being submitted to the ischemia/reperfusion procedure; Ischemic postconditioning (Post) - rats submitted to three cycles of clamping and releasing the aorta for one min after the 240-minute ischemic phase; Ischemic pre and postconditioning (Pre-post) - animals submitted to the same procedures of Pre and Post groups. Skeletal muscle injury was evaluated by measuring serum levels of aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK); and muscular levels of malondialdehyde (MDA) and glycogen. RESULTS: AST levels were significantly higher in Pre and Pre-post groups (P<.01). There were no differences in LDH and CPK levels. Muscular MDA levels were similar. Glycogen levels were significantly higher in Pre and Pre-post groups (P<.01). CONCLUSIONS: Both preconditioning and its association with postconditioning had a protective effect by avoiding glycogen depletion in skeletal muscle in rats submitted to ischemia and reperfusion. Association of pre and postconditioning did not show advantage compared to preconditioning alone. Postconditioning alone did not show protective effect.
Abstract in English:PURPOSE: To investigate the correlation between bursting pressure and breaking strength on the 7th postoperative day following left colonic anastomosis in rats. METHODS: Seventy rats were randomly divided into seven groups of ten animals each. All of the animals underwent segmental resection of the left colon and end-to-end anastomosis. The animals in groups I to VI underwent surgical laparoscopies with pneumoperitoneums using carbon dioxide or helium at pressures of 5, 12 or 20 mmHg. In Group VII, open laparotomy was performed. The animals were reoperated on postoperative day 7 to measure the bursting pressure and the breaking strength of the anastomosis. RESULTS: The anastomosis bursting pressure in 70 animals was 193.10±55.56 mmHg. There was no significant difference between the groups (p=0.786). The breaking strength of the anastomosis was 0.26±0.12 N. There was no significant difference between the groups (p=0.356). Pearson's correlation test showed a low correlation (r=0.231) lacking statistical significance (p=0.054). CONCLUSION: There was no correlation between the bursting pressure and breaking strength of left colonic anastomoses in rats on the 7th postoperative day.
Abstract in English:PURPOSE:To assess whether late introduction of a specific COX-2 inhibitor (Meloxicam) can treat and/or prevent the progression of tumors in the stomach of rats submitted to duodenogastric reflux. METHODS: Seventy five male Wistar rats, weighing 150 grams, were submitted to the induction of duodenogastric reflux through the pylorus. At 36 weeks of follow-up were established three experimental groups: DGR36 sacrificed immediately, DGR54 and DGR54MLX both sacrificed at 54th week of follow-up . The animals of the latter group were fed with a rat chow premixed with Meloxicam (2.0 mg/ kg feed; 0.3 mg / kg bw / day) and the other two with standard rat chow. The lesions found in the pyloric mucosa and gastrojejunal anastomosis were analyzed macroscopically and histologically. For statistical analysis was adjusted a generalized linear model assuming a binomial distribution with LOGIT link function. RESULTS: No significant differences were found when comparing the incidences of benign tumor lesions (Adenomatous Hyperplasia), p=0.4915, or malignant (Mucinous Adenocarcinoma), p=0.2731, among groups. CONCLUSION: Late introduction of specific COX-2 inhibitor (Meloxicam) did not treat and was not able to prevent the progression of tumoral lesions induced by duodenogastric reflux in the rat stomachs.
Abstract in English:PURPOSE: To investigate if tests used in the preoperative period of upper abdominal or thoracic surgeries are able to differentiate the patients that presented cardiopulmonary postoperative complications. METHODS: Seventy eight patients, 30 submitted to upper abdominal surgery and 48 to thoracic surgery were evaluated. Spirometry, respirometry, manovacuometry, six-minute walk test and stair-climbing test were performed. Complications from immediate postoperative to discharge from hospital were registered. RESULTS: The postoperative complications rate was 17% in upper abdominal surgery and 10% in thoracic surgery. In the univariate regression, the only variable that kept the correlation with postoperative complications in the upper abdominal surgery was maximal expiratory pressure. In thoracic surgery, the maximal voluntary ventilation, six-minute walk test and time in stair-climbing test presented correlation with postoperative complications. After multiple regression only stair-climbing test continued as an important risk predictor in thoracic surgery. CONCLUSION: The respiratory pressure could differentiate patients with complications in upper abdominal surgery, whereas in thoracic surgery, only spirometric values and exercise tests could differentiate them.
Abstract in Portuguese:OBJETIVO: Avaliar titulação e produção de docentes da área cirúrgica das Universidades Estadual do Pará (UEPA), Federal do Pará (UFPA) e Federal da Paraíba (UFPB). MÉTODOS: A produção dos docentes nos últimos cinco anos foi classificada segundo o Qualis da Medicina III da CAPES (http://qualis.capes.gov.br/webqualis/publico/pesquisaPublicaClassificacao.seam?conversationPropagation=begin), e computou-se o número de orientações concluídas. RESULTADOS: Foram incluídos 74 docentes, 31 mestres e 43 doutores. A UFPA apresentou maior produção científica (49% do total de publicações), com 32% destas em periódicos Qualis B1 ou superior. A especialidade com maior número de publicações foi a Otorrinolaringologia (29% do total); entretanto, 98% destas em Qualis B3 ou inferior. Já a Cirurgia Gastroenterológica, responsável por 23% do total, teve 57% de suas publicações em Qualis B1 ou superior. O tipo mais frequente de orientação foi a de trabalhos de conclusão de cursos de graduação (63%). Ginecologia e Obstetrícia apresentou o maior número de orientações concluídas (35% do total), e Cirurgia Gastroenterológica o maior número de orientações de mestrado e doutorado. CONCLUSÕES: As especialidades Ginecologia e Obstetrícia e Cirurgia Gastroenterológica apresentaram o maior número de docentes titulados. A Cirurgia Gastroenterológica apresentou a maior produção nos extratos do Qualis-CAPES, e também o maior número de orientações concluídas de mestrado e doutorado.
Abstract in English:PURPOSE: To evaluate the scientific production from the surgical areas from the State University of Para (UEPA), Federal Universities of Para (UFPA) and of Paraiba (UFPB), Brazil. METHODS: The scientific productions of professors in the last five years were classified according to the Qualis system of CAPES-Medicine III (http://qualis.capes.gov.br/webqualis/publico/pesquisaPublicaClassificacao.seam?conversationPropagation=begin) and it was computed the number of guidance of students scientific projects. RESULTS: Seventy-four professors were included, 31 with Master and 43 with PhD degrees. UFPA presented more publications (49% out of the total), which 32% of them in Journals B1 or higher. Otorhinolaryngology presented the greatest number of publications (29% out of the total); however, 98% of them in B3 or lower. Gastroenterological Surgery, which is responsible for 23% out of the total, had 57% of its publications in B1 or higher. The most frequent type of guidance was the one about scientific work of completion of undergraduate course (63%). Gynecology and Obstetrics presented the greatest number of student's guidance (35% out of the total) and Gastroenterological Surgery the largest number of Master's degree and PhD degree supervisions. CONCLUSIONS: The specialties Gynecology and Obstetrics and Gastroenterological Surgery from the State University of Para (UEPA), Federal Universities of Para (UFPA) and of Paraiba (UFPB) presented the greatest number of professors with academic degrees. Gastroenterological Surgery presented the largest scientific productions at higher strata of WebQualis CAPES classification and also the largest number of Master and PhD directed.