Cells of Saccharomyces carlsbergensis, (IZ-273) were grow in esterilized sugar cane juice medium whith 0, 0,001%, 0,002%, 0,003% e 0,005% of Emulsan Al. In Warburg apparatus the yeast cells fermented: glucose, sugar cane juice, sucrose and rafinose, and did not ferment: galactose, maltose and lactose. These showed a very slow endogenous fermentation. For glucose and sugar cane juice the cells from medium with Emulsan Al demonstrated a considerable yeld. The rate of fermentation of rafinose was strongly increased with cells from the medium with Emulsan Al (2 - 3 times faster).