The influence of three kinds of a different speacially mixed substrates, namely: (a) sand + loam + chicken manure; (b) sand + loam + cattle manure; and (c) sand + loam, and foliar application of water as needed and nutrient solution at 15 and 30 days intervals, in the nutrient extraction amount by seedling of guaraná (Paullinia cupana var. sorbilis (Mart.) Ducke), were verified by means of a pot experiment, in Amazonas State. Nitrogen was most required, followed by K, Ca, Mg, P and S. In the cattle manure substrate the seedlings showed most nutrients extraction. The organic matter nutrient content, except N, were suficient for the seedlings nutrition.