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Movimento do protoplasma nos pêlos internos do tubo da corola de Allamanda cathartica, Linn

A new example of protoplasmic streaming occuring in the hairs attached to the inner wall and just above the anthers of corolla tube of Allamanda cathartica is described. There are five fascicles of hyalin and unicellular hairs, one for each stamen; they are united together, forming a conical protection over the anthers. The following procedure is recommended for microscopical examinations: it is necessary to remove most of the corolla as far as the level of the cone of hairs, and then the tube is divided in to five parts; under stereoscopic lupe, thin tube sections are obtained with a scalpel, lanceolated needle or razor blade, and the sections with a few hairs are mounted in water or liquid vaseline. In order to obtain good slides it is important to remove the air kept among the hairs with warm water, ten per cent ethyl alccohl or liquid vaseline. The only inconvenient presented by the use of liquid vaseline is some refringece around the hairs. The protoplasmic streamings are very active, but currents have not always the same course along the hairs; many time they change their directions and the cytoplasmic flows may be rapid or evenrushy in a protoplasmic strands. The bodies called "microsomes", which are suspended in the protoplasm, in most cases are carried along more or less rapidly by the currents. The observed movements belong to the gyratory, and ondulatory types.


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