Efeito do ácido 2,4-diclorofenoxiacético (2,4-D) na absorção do fósforo (32p) pelo trigo (Triticum aestivum., L) e a sua distribuição na planta

Alterman, Marcelo Kogan; Neptune, André Martin Louis

doi:10.1590/S0071-12761977000100042

Resumos

O objetivo deste trabalho foi estudar o efeito de diferentes concentrações de 2,4-D na absorção de fósforo - pelo trigo (Triticum aestivum, L) no estado de 3 folhas e a sua distribuição na planta. Foram realizados quatro ensaios em vaso, conduzidos em casa de vegetação, com quatro repetições. As plantas foram cortadas 8 horas, 26 e 50 horas após a pulverização do herbicida. Em cada um dos ensaios foram aplicados 4 doses de 2,4-D: 0, 1000, 2000 e 4000 ppm e uma solução contendo 32p. Os dados obtidos mostraram que: - 0, 2,4-D afetou a absorção do fósforo e a distribuição deste na planta. - A tendência, nos quatro ensaios, foi de que as doses utilizadas estimularam a absorção do fósforo pelo trigo, a qual foi decrescendo com o decorrer do tempo. - A distribuição do fósforo foi, de modo geral, estimulada, decrescendo com o decorrer do tempo. No terceiro ensaio, quando as plantas foram pulverizadas com 2000 e 4000 ppm. de 24-D, a distribuição foi prejudicada, sendo maior naquelas plantas não tratadas com o herbicida.

The aim of this paper was to study the effect of different concentrations of 2,4-D on the phosphorus uptake by wheat plants (Triticum aestivum, E.) at the 3 leaves stage and its distribution in the plants. Four experiments were carried out in the greenhouse, with four replicates. In the first experiment the wheat plants were harvested 8 h hours after spraying the herbicide. In the second, third and fourth experiments, after 14, 26 and 50 hours respectively. In each experiment, four levels of 2,4-D, namely, 0, 1000, 2000, and 4000 ppm. and a solution with 32P were applied. The data showed: - The 2,4-D affected the phosphorus uptake and its distribution in the plant. - The four experiments showed a tendency toward 2,4-D stimulation of phosphorus uptake in wheat, which decreased with the passage of time. - The phosphorus translocation to the shoot was, in general, stimulated, but this decreased with time. In the third experiment when the plants were sprayed with 2000and 4000 ppm. 2,4-D, the translocation was reduced with respect to the control plants.

Marcelo Kogan Alterman^I; André Martin Louis Neptune^II

^IUniversidade do Chile - Faculdade de Agronomia

^IICentro de Energia Nuclear na Agricultura e Departamento de Solos, Geologia e Fertilizantes. Escola Superior de Agricultura "Luiz de Queiroz" - Universidade de São Paulo

RESUMO

O objetivo deste trabalho foi estudar o efeito de diferentes concentrações de 2,4-D na absorção de fósforo - pelo trigo (Triticum aestivum, L) no estado de 3 folhas e a sua distribuição na planta.

Foram realizados quatro ensaios em vaso, conduzidos em casa de vegetação, com quatro repetições. As plantas foram cortadas 8 horas, 26 e 50 horas após a pulverização do herbicida. Em cada um dos ensaios foram aplicados 4 doses de 2,4-D: 0, 1000, 2000 e 4000 ppm e uma solução contendo ³²p.

Os dados obtidos mostraram que: - 0, 2,4-D afetou a absorção do fósforo e a distribuição deste na planta.

- A tendência, nos quatro ensaios, foi de que as doses utilizadas estimularam a absorção do fósforo pelo trigo, a qual foi decrescendo com o decorrer do tempo.

- A distribuição do fósforo foi, de modo geral, estimulada, decrescendo com o decorrer do tempo. No terceiro ensaio, quando as plantas foram pulverizadas com 2000 e 4000 ppm. de 24-D, a distribuição foi prejudicada, sendo maior naquelas plantas não tratadas com o herbicida.

SUMMARY

The aim of this paper was to study the effect of different concentrations of 2,4-D on the phosphorus uptake by wheat plants (Triticum aestivum, E.) at the 3 leaves stage and its distribution in the plants.

Four experiments were carried out in the greenhouse, with four replicates. In the first experiment the wheat plants were harvested 8 h hours after spraying the herbicide. In the second, third and fourth experiments, after 14, 26 and 50 hours respectively. In each experiment, four levels of 2,4-D, namely, 0, 1000, 2000, and 4000 ppm. and a solution with ³²P were applied.

The data showed:

- The 2,4-D affected the phosphorus uptake and its distribution in the plant.

- The four experiments showed a tendency toward 2,4-D stimulation of phosphorus uptake in wheat, which decreased with the passage of time.

- The phosphorus translocation to the shoot was, in general, stimulated, but this decreased with time. In the third experiment when the plants were sprayed with 2000and 4000 ppm. 2,4-D, the translocation was reduced with respect to the control plants.

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LITERATURA CITADA

Recebido para publicação em 30-12-1977.

ANDREA E., A. AY. 1963. Herbicides. In Metabolic inhibitors (Ed. by R.H., Hoscher e J.H. Quastel) Vol. II, pp. 243-261. Academic Press, New York, London.
AUDUS, E.J. 1963. Plant growth substances. London, Leonard Hill Book limited. Interscience publishers. Inc. New York. Second Editions. 553 p.
AWERBUCH, T. & AVNIMELECH, Y. 1970. Conting ³²P in plant tissues using the Cerenkov effect. Plant and Soil, 33, 260-264.
BRADRURY, D. e B.W. ENNIS. 1952. Stomacal closure in kidney bean plant treated with ammonium 2,4-dichlorophenoxyacetic. Am. J. Bot., 39, 324-328.
BRIAN, C.R. 1964. The effects of herbicides on biophysical processes in the plant. In The phisiology and biochemistry of herbicides. (Ed. by. J.L. Audus) pp. 357-386. Academic Press, London, New York.
CMKANIKOV, I.D.; O.D. MIRITYUK; A.M. MAKEEV e Y.U.M. MIRENKOV. 1970. Additional data on disturbance of phosphorylative processes under the influence of 2,4-D. Societ Plant Physiol., 17, 633-639.
COOKE, A.R. 1957. Influence of 2,4-D on the uptake of minerals from the soil. Weeds, 5, 25-28.
ELLIS, K.M.; S.M. WAMPLER e R.H. YAGER. 1966. Liquid Scintillation method for determination of solvent extracted phosphorus - 32 in foods. Anl. chim. Acta., 34, 1969-1974.
FERNANDEZ, A.O. 1963. Acción fisiológica del acido 2,4 diclorofenoxyacético. Revist. de la Facultad de Agronomia Y Veterinária de Buenos Aires. Tomo XV, 11-20.
FREED, V.H., J.F. REITHEL e F.L. REMMERT. 1961. Some physical chemical aspects of synthetic auxins whith respect to their mode of action. Plant Growth Regulation. Proc. 4th. Int. Conf., 289-303. Iowa State University Pres, Iowa, U.S.A.
GUINEA, L. e C. CARAMETE. 1970. Influenta erbicidelor asupra metamolismului plantelor. Probleme Agricole, 11, 42-49.
HABERER, K. 1966. Measurement of beta activities in aqueous samples utilizing Cerenkov radiation. Tecnical Bulletin Packard Instrument Company, Inc. N° 16. 14 pp.
HAGIN, D.R., D.L. LINSCOTT e J.E. DAWSON. 1970. 2,4-D metabolism en resistant grasses. J. Agr. Food Chem., 18, 848-850.
KEY, L.J.; J.B. HANSON e R.F. BILS. 1960. Effect of 2,4 dichlorophenoxyacetic acid application on activity and composition of mitocondria soybeans. Plant Physiol., 35, 177-183.
KOGAN, A.M. 1971. Effecto del herbicida 2,4 diclorofenoxyacético (2,4-D) en los principales processos metabolicos de las plantas. Boletim Didático 003, Centro de Energia Nuclear na Agricultura, Escola Superior de Agricultura "Luiz de Queiroz" assimilates in red maple and white ash. Bot. Gaz. 127, 193-201.
LEONARD, O.A.; E.D. BAYER e J.K.R. GLENN. 1966. Translocation of labelled U.S.P. 40 pp.
MACIEJENSKA - POTAPCZYK, V. 1955. The action of 2,4-D on some of the enzymes of the stomacal cells. Acta. Soc. Bot. Pol., 24, 639-645.
MORELALND, D. E. 1967. Mechanism of action of herbicides. Ann. Rev. Plant Physiol., 18, 365-368.
NORMAN, G. A.; C.E. MINARTK. 1950. Herbicides. Ann. Rev. of Plant PhvsioL, 1, 141-168.
PARMENTIER, H.J., e F.E.L. TENHAAF. 1969. Developments in liquid scintillation counting since 1963. The internacional Journ. of applied radiation and isotopes, 20, 305-334.
ROBERTSON, M.M. e R.C. KIRDWOOD. 1970. The mode of action of foliage applied translocated herbicides with particular reference to the phenoxy - acid compounds. II The mechanism and factors influencing translocation, metabolism and biochemical inhibition. Weed Res., 10, 94-120.
ROBINSON, R.J. 1969. ³³p: a superior radiation for phosphorus? The International journ. of Applied Radiation and Isotopes, 20, 531-534.
SASAKI, S. e T.T. KOZLOWSKL 1967. Effects of herbicids on carbon dioxide uptalke by pine seedlings. Can. J. Bot., 45, 961-971.
SWJTZER, C.M. 1957. Effect of herbicides and related chemicals on oxidation and phosphorylation buy isolated soybeans mitocondria. Plant Physiol., 32, 42-4-1.
TURNER, C.J. s/data. Sample preparation for liquid cintillation techniques. The Radiochemical Centre Amersham, England, Review 6, 31 pag.
WEDDING, T.R. e M.K. BLACK. 1962. Response of oxidation and coupled phosphorylation in plant mitochondriato 2,4-dichorophenoxycetic acid. Plant Physiol., 37, 364-370.
WHITE, P.R. e G.B. ELLIS. 1968. Routine couting of ³²P in colored solutions from dry ashed plant samples utilizing Cerenkov radiation. Proc. Soil Sci. Soc. Am., 32, 749-751.
WORT, D.J. 1964a. Effects of herbicides on plant composition and metabolism. In The Physiology and Biochemistry of Herbicides. (Ed by L.J. Audus), pp. 291-334. Academic Press, London and New York.
YUFERA, P.E. 1958. Herbicidas y Fitorreguladores. Madrid, Espafia, Ed. Aguilar.
ZIMMERMAN P.W. e E. A. HITCHCOK. 1942. Substituted phenoxy and benzoic acid growth substances and the relation of structure to physiological activity. Coritr. Boyce Thompson Inst., 12, 32L343.

**Efeito do ácido 2,4-diclorofenoxiacético (2,4-D) na absorção do fósforo (³²p) pelo trigo (Triticum aestivum., L) e a sua distribuição na planta**

Effects of 2,4-D in the uptake of phosphorus (³²p) by wheat (Triticum aestivum, L) and its distribution in the plant

Datas de Publicação

Publicação nesta coleção
17 Maio 2012
Data do Fascículo
1977

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] ANDREA E., A. AY. 1963. Herbicides. In Metabolic inhibitors (Ed. by R.H., Hoscher e J.H. Quastel) Vol. II, pp. 243-261. Academic Press, New York, London.

[2] AUDUS, E.J. 1963. Plant growth substances. London, Leonard Hill Book limited. Interscience publishers. Inc. New York. Second Editions. 553 p.

[3] AWERBUCH, T. & AVNIMELECH, Y. 1970. Conting ³²P in plant tissues using the Cerenkov effect. Plant and Soil, 33, 260-264.

[4] BRADRURY, D. e B.W. ENNIS. 1952. Stomacal closure in kidney bean plant treated with ammonium 2,4-dichlorophenoxyacetic. Am. J. Bot., 39, 324-328.

[5] BRIAN, C.R. 1964. The effects of herbicides on biophysical processes in the plant. In The phisiology and biochemistry of herbicides. (Ed. by. J.L. Audus) pp. 357-386. Academic Press, London, New York.

[6] CMKANIKOV, I.D.; O.D. MIRITYUK; A.M. MAKEEV e Y.U.M. MIRENKOV. 1970. Additional data on disturbance of phosphorylative processes under the influence of 2,4-D. Societ Plant Physiol., 17, 633-639.

[7] COOKE, A.R. 1957. Influence of 2,4-D on the uptake of minerals from the soil. Weeds, 5, 25-28.

[8] ELLIS, K.M.; S.M. WAMPLER e R.H. YAGER. 1966. Liquid Scintillation method for determination of solvent extracted phosphorus - 32 in foods. Anl. chim. Acta., 34, 1969-1974.

[9] FERNANDEZ, A.O. 1963. Acción fisiológica del acido 2,4 diclorofenoxyacético. Revist. de la Facultad de Agronomia Y Veterinária de Buenos Aires. Tomo XV, 11-20.

[10] FREED, V.H., J.F. REITHEL e F.L. REMMERT. 1961. Some physical chemical aspects of synthetic auxins whith respect to their mode of action. Plant Growth Regulation. Proc. 4th. Int. Conf., 289-303. Iowa State University Pres, Iowa, U.S.A.

[11] GUINEA, L. e C. CARAMETE. 1970. Influenta erbicidelor asupra metamolismului plantelor. Probleme Agricole, 11, 42-49.

[12] HABERER, K. 1966. Measurement of beta activities in aqueous samples utilizing Cerenkov radiation. Tecnical Bulletin Packard Instrument Company, Inc. N° 16. 14 pp.

[13] HAGIN, D.R., D.L. LINSCOTT e J.E. DAWSON. 1970. 2,4-D metabolism en resistant grasses. J. Agr. Food Chem., 18, 848-850.

[14] KEY, L.J.; J.B. HANSON e R.F. BILS. 1960. Effect of 2,4 dichlorophenoxyacetic acid application on activity and composition of mitocondria soybeans. Plant Physiol., 35, 177-183.

[15] KOGAN, A.M. 1971. Effecto del herbicida 2,4 diclorofenoxyacético (2,4-D) en los principales processos metabolicos de las plantas. Boletim Didático 003, Centro de Energia Nuclear na Agricultura, Escola Superior de Agricultura "Luiz de Queiroz" assimilates in red maple and white ash. Bot. Gaz. 127, 193-201.

[16] LEONARD, O.A.; E.D. BAYER e J.K.R. GLENN. 1966. Translocation of labelled U.S.P. 40 pp.

[17] MACIEJENSKA - POTAPCZYK, V. 1955. The action of 2,4-D on some of the enzymes of the stomacal cells. Acta. Soc. Bot. Pol., 24, 639-645.

[18] MORELALND, D. E. 1967. Mechanism of action of herbicides. Ann. Rev. Plant Physiol., 18, 365-368.

[19] NORMAN, G. A.; C.E. MINARTK. 1950. Herbicides. Ann. Rev. of Plant PhvsioL, 1, 141-168.

[20] PARMENTIER, H.J., e F.E.L. TENHAAF. 1969. Developments in liquid scintillation counting since 1963. The internacional Journ. of applied radiation and isotopes, 20, 305-334.

[21] ROBERTSON, M.M. e R.C. KIRDWOOD. 1970. The mode of action of foliage applied translocated herbicides with particular reference to the phenoxy - acid compounds. II The mechanism and factors influencing translocation, metabolism and biochemical inhibition. Weed Res., 10, 94-120.

[22] ROBINSON, R.J. 1969. ³³p: a superior radiation for phosphorus? The International journ. of Applied Radiation and Isotopes, 20, 531-534.

[23] SASAKI, S. e T.T. KOZLOWSKL 1967. Effects of herbicids on carbon dioxide uptalke by pine seedlings. Can. J. Bot., 45, 961-971.

[24] SWJTZER, C.M. 1957. Effect of herbicides and related chemicals on oxidation and phosphorylation buy isolated soybeans mitocondria. Plant Physiol., 32, 42-4-1.

[25] TURNER, C.J. s/data. Sample preparation for liquid cintillation techniques. The Radiochemical Centre Amersham, England, Review 6, 31 pag.

[26] WEDDING, T.R. e M.K. BLACK. 1962. Response of oxidation and coupled phosphorylation in plant mitochondriato 2,4-dichorophenoxycetic acid. Plant Physiol., 37, 364-370.

[27] WHITE, P.R. e G.B. ELLIS. 1968. Routine couting of ³²P in colored solutions from dry ashed plant samples utilizing Cerenkov radiation. Proc. Soil Sci. Soc. Am., 32, 749-751.

[28] WORT, D.J. 1964a. Effects of herbicides on plant composition and metabolism. In The Physiology and Biochemistry of Herbicides. (Ed by L.J. Audus), pp. 291-334. Academic Press, London and New York.

[29] YUFERA, P.E. 1958. Herbicidas y Fitorreguladores. Madrid, Espafia, Ed. Aguilar.

[30] ZIMMERMAN P.W. e E. A. HITCHCOK. 1942. Substituted phenoxy and benzoic acid growth substances and the relation of structure to physiological activity. Coritr. Boyce Thompson Inst., 12, 32L343.

Brasil

Brasil

Efeito do ácido 2,4-diclorofenoxiacético (2,4-D) na absorção do fósforo (32p) pelo trigo (Triticum aestivum., L) e a sua distribuição na planta

Effects of 2,4-D in the uptake of phosphorus (32p) by wheat (Triticum aestivum, L) and its distribution in the plant

Resumos

**Efeito do ácido 2,4-diclorofenoxiacético (2,4-D) na absorção do fósforo (³²p) pelo trigo (Triticum aestivum., L) e a sua distribuição na planta**

Datas de Publicação