Desenvolvimento e produção de seda do Bombyx mori L. exposto a análogos do hormônio juvenil

Miranda, J.E.; Bortoli, S.A. De; Takahashi, R.

Resumos

O crescimento dos insetos é regulado por um complexo hormonal que inclui o hormônio juvenil, que assegura ao inseto a retenção de suas características larvais, prevenindo a maturação. A aplicação de análogos do hormônio juvenil em Bombyx mori tem promovido o incremento da produção de seda; entretanto, altas doses podem provocar efeitos deletérios sobre a biologia do inseto. Este estudo avaliou a pulverização de diferentes análogos do hormônio juvenil sobre o bicho-da-seda e seus efeitos sobre o desenvolvimento larval e a produção de seda. Lagartas foram expostas a piriproxifeno, metopreno e fenoxicarbe a doses de 20, 2 e 0,02 ppb de ingrediente ativo, respectivamente, através da pulverização dos insetos e do alimento a 48 horas após a quarta ecdise. A administração de análogos do hormônio juvenil promoveu aumentos no período larval, massa de glândulas sericígenas, peso de casulos e de pupas. Os produtos testados afetaram negativamente a taxa de encasulamento. Metopreno promoveu o maior incremento na produção de seda e menor taxa de mortalidade, podendo seu uso ser recomendado na sericicultura.

Sericicultura; piriproxifeno; metopreno; fenoxicarbe; neotenin

Insect growth is regulated by a hormonal complex that includes the juvenile hormone, which assures to the insect the retention of its larval characteristics, preventing maturation. Applications of juvenile hormone analogues on Bombyx mori have induced increases in silk production; however, high doses may promote deleterious effects on the biology of the insect. The present study evaluated the spraying of different juvenile hormone analogues on the silkworms and their effects on the larval development and silk production. Larvae were exposed to piryproxyfen, methoprene and fenoxycarb at titers of 20, 2 and 0.02 ppb a.i., respectively, sprayed on the insects and food at 48 hours after the fourth ecdysis. The administration of juvenile hormone analogues promoted increases in the larval period, silk glands, cocoon and pupae weight. The tested products had a negative effect on the cocooning rate. Methoprene promoted a larger increment in the silk production and smaller fall in the cocooning rate, and its use can be recommended in sericulture.

Sericulture; pyriproxyfen; methoprene; fenoxycarb; neotenin

AHMAD, I.; RAHAYU, R.; AGUS, D.; PERMANA, D.; ASTARI, S. Alteration of ecdysteroid titre by thyroxine and juvenile hormone analogue (methoprene) in Bombyx mori (Lepidoptera: Bombycidae). Biota, v.12, n.2, p.116-121, 2007.
AKAI, H.; KIGUCHI, K.; MORI, K. Increased accumulation of silk protein accompanies KJH-induced prolongation of larval life in Bombyx mori L. Applied Entomology and Zoology, v.6, n.2, p.218-220, 1971.
AKAI, H.; TAKABAYASHI, K.; KIUCHI, M. Induction of spinning from JH-treated larvae of Bombyx mori by ecdysteroid administration. Journal of Sericultural Science of Japan, v.57, n.4, p.341-344, 1988.
CAPPELLOZZA, L.; CAPPELLOZZA, S.; SBRENNA, G. Changes in the developmental programme of Bombyx mori caused by oral treatment with fenoxycarb during the last larval instar. Sericologia, v.35, n.3, p.427-436, 1995.
CAPPELLOZZA, L.; IANNE, P.; CAPPELLOZZA, S. Effect of body weight on effectiveness of the insect growth regulator (I. G. R.) fenoxycarb applied to the male and female silkworm (Bombyx mori) (Lepidoptera: Bombycidae). Sericologia, v.37, n.3, p.443-452, 1997.
CHANG, E.S. Comparative endocrinology of molting and reproduction: Insects and crustaceans. Annual Review of Entomology, v.38, n.2, p.161-180, 1993.
CHOWDHARY, S.K.; SEHNAL, F.; RAJ, S.K.; RAJU, P.S.; MATHU, S. Giant cocoon formation in Bombyx mori L. topically treated with juvenile hormone SJ-42-F. Sericologia, v.26, n.4, p.455-459, 1986.
CHOWDHARY, S.K.; RAJU, P.S.; OGRA, R.K. Effect of JH analogues on silkworm, Bombyx mori L., growth and development of silk gland. Sericologia, v.30, n.2, p.156-165, 1990.
CIFUENTES, C.C.A.; SOHN, K.W. Manual técnico de Sericicultura: cultivo de la morera y cría del gusano de seda en el trópico. Pereira: Fundo Editorial de Risaralda, 1998. 438p.
DEDOS, S.G.; FUGO, H. Disturbance of adult eclosion by fenoxycarb in the silkworm, Bombyx mori. Journal of Insect Physiology, v.45, n.2, p.257-264, 1999.
EWER, J.; GAMMIE, S.C.; TRUMAN, J.W. Control of insect ecdysis by a positive-feedback endocrine system: role of eclosion hormone and ecdysis triggering hormone. Journal of Experimental Biology, v.200, n.5, p.869-881, 1997.
GAABOUB, I.A.; EL-HELALY, M.S.; MOSTAFA, S.M. Food utilization, rate of larval growth, and fecundity of Bombyx mori L. (Lepidoptera: Bombycidae) fed mulberry leaves treated with methoprene, triprene, and diflubenzuron. Journal of Economical Entomology, v.78, n.6, p.1182-1186, 1985.
GANGWAR, S.K. Effect of juvenile hormone mimic R394 on silkworm (Bombyx mori L.) growth and development of silk gland. Journal of Agricultural and Biological Science, v.4, n.6, p.65-67, 2009.
ISHAAYA, K.; ASCHER, K.R.S. Effect of diflubenzuron on growth and carbohydrate hydrolases of Tribolium castaneum. Phytoparasitica, v.5, n.1, p.149-158, 1977.
ISHAAYA, I.; HOROWITZ, R. Pyriproxyfen, a novel insect growth regulator for controlling whiteflies: mechanisms and resistance management. Pesticide Science, v.43, n.1, p.227-232, 1995.
KAJIURA, Z.; KADONO-OKUDA, K.; YAMASHITA, O. Induction of dauer larvae by a juvenile hormone analogue and their response to ecdysteroids in the silkworm, Bombyx mori. Journal of Sericultural Science of Japan, v.56, n.5, p.398-406, 1987.
KAJIURA, Z.; YAMASHITA, O. Super growth of silkglands in the dauer larvae of the silkworm, Bombyx mori, induced by a juvenile hormone analogue. Journal of Sericultural Science of Japan, v.58, n.1, p.39-46, 1989.
KAMIMURA, M. Effects of a juvenile hormone analogue, fenoxycarb, on larval growth of the silkworm, Bombyx mori (Lepidoptera: Bombycidae). Applied Entomology and Zoology, v.30, n.3, p.487-489, 1995.
KING, D.S.; SIDDALL, J. Conversion of α-ecdysone to β-ecdysone by crustaceans and insects. Nature, v.221, n.5, p.955-956, 1969.
KOTIKAL, Y.; DEVAIAH, M.C. Juvenile hormones in sericulture. Indian Silk, n.25, p.19-20, 1986.
KURATA, K.; DAILLIE, J. Effect of exogenous juvenoid on the growth of the silkgland and the synthesis of nucleic acid and silk protein by the silkgland of Bombyx mori. Bulletin of Sericulture Experimental Station of Japan, v.27, n.4, p.507-530, 1978.
LEONARDI, M.G.; CAPPELLOZZA, S.; IANNE, P.; CAPPELLOZZA, L.; PARENTI, P.; GIORDANA, B. Effects of the topical application of an insect growth regulator (fenoxycarb) on some physiological parameters in the fifth instar larvae of the silkworm Bombyx mori. Compendium of Biochemistry and Physiology, v.113B, n.2, p.361-365, 1996.
MAMATHA, D.M.; KANJII, V.K.; COHLY, H.H.P.; RAO, M.R. Juvenile hormone analogues, methoprene and fenoxycarb dose-dependently enhance certain enzyme activities in the silkworm Bombyx mori L. Environmental Research and Public Health, v.5, n.2, p.120-124, 2008.
McNABB, S.L.; TRUMAN, J.W. Light and peptidergic eclosion hormone neurons stimulate a rapid eclosion response that masks circadian emergence in Drosophila. Journal of Experimental Biology, v.211, p.2263-2274, 2008.
MENEGUIM, A.M.; LOVATO, L.; SILVA, R.Z. da; YAMAOKA, R.S.; NAGASHIMA, G.T.; PASINI, A. Influência de cultivares de amoreira Morus spp. sobre a produção e qualidade de casulos de bicho-da-seda, Bombyx mori L. (Lepidoptera: Bombycidae). Neotropical Entomology, v.36, n.5, p.670-674, 2007.
MIRANDA, J.E.; BORTOLI, S.A.; TAKAHASHI, R. Development and silk production by silkworm larvae after topical application of methoprene. Scientia Agricola, v.59, n.3, p.585-588, 2002a.
MIRANDA, J.E.; BORTOLI, S.A.; TAKAHASHI, R.; SILVA, A.F. Efeito de três análogos do hormônio juvenil aplicados em bicho-da-seda, Bombyx mori L. (Lepidoptera: Bombycidae). Neotropical Entomology, v.31, n.1, p.19-25, 2002b.
MIRANDA, J.E.; BORTOLI, S.A.; TAKAHASHI, R.; SILVA, A.F. Índices nutricionais de bicho-da-seda (Bombyx mori L.) tratados com análogos do hormônio juvenil. Revista Científica Rural, v.8, n.1, p.32-38, 2003.
RADHAKRISHNA, P.G.; DELVI, M.R. Effect of organophosphorous insecticides on food utilization in different races of Bombyx mori (Lepidoptera: Bombycidae). Sericologia, v.32, n.1, p.71-79, 1992.
RIDDIFORD, L.M.; CHERBAS, P.; TRUMAN, J.W. Ecdysone receptors and their biological actions. Vitamins and Hormones, v.60, n.1, p.1-73, 2000.
SAKURAI, S.; OKUDA, M; OHTAKI, T. Juvenile hormone inhibits ecdysone secretion and responsiveness to prothoracic glands of Bombyx mori. Genetic Comparative Endocrinology, v.75, n.2, p.222-230, 1989.
SARANGI, S.K. Effect of juvenile hormone analogue on the silk gland of the silkworm, Bombyx mori L. Sericologia, v.28, n.4, p.553-557, 1988.
SCHNEIDERMAN, H.A.; GILBERT, L.I. Control of growth and development in insects. Science, n.143, p.325-333, 1964.
SCRIBER, J.M.; SLANSKY JUNIOR, F. The nutritional ecology of immature insects. Annual Review of Entomology, v.26, n.1, p.183-211, 1981.
STAAL, G. Insect control with growth regulators interfering with the endocrine system. Entomologia Experimentalis et Applicata, v.31, n.1, p.15-23, 1982.
SUBBA RAO, G.; DAS, S.K.; NANDI, S.; ROMPOMU R.; SEN, S.K. A study on the effect of a juvenile hormone analogue on the bivoltine silkworm, B. mori L. Sericologia, v.28, n.4, p.543-564, 1988.
TAKAHASHI, R.; TAKAHASHI, K.M.; TAKAHASHI, L.S. Sericicultura: uma promissora exploração agropecuária Jaboticabal: Editora Funep, 2001. 126p.
TSUKADA, M.; KIMURA, K.; SHIBUKAWA, A.; SHIMAZAKI, A.; AKAI, H. Effect of treatment with juvenile hormone analogue and anti-juvenoid on the size of the cocoon filament. Journal of Sericultural Science of Japan, v.56, n.3, p.231-234, 1987.
WATANABE, J.K.; YAMAOKA, R.S.; BARONI, S.A. Cadeia produtiva da seda: diagnósticos e demandas atuais Londrina: IAPAR, 2000. 129p. (IAPAR, Documentos, 22).
UNNI, B.G.; BHATTACHARJEE, M.; GOSWANI, A.M.; CHUTIA, S.B.; DAS, S.; RAJKHOWA, G.; KAKOTY, Y. Role of insect neuropeptides and juvenile hormones in silk protein biosynthesis. Annals of Neurosciences, v.15, n.4, p.112-117, 2008.

Datas de Publicação

Publicação nesta coleção
10 Set 2014
Data do Fascículo
Mar 2012

Histórico

Recebido
17 Ago 2010
Aceito
14 Mar 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AHMAD, I.; RAHAYU, R.; AGUS, D.; PERMANA, D.; ASTARI, S. Alteration of ecdysteroid titre by thyroxine and juvenile hormone analogue (methoprene) in Bombyx mori (Lepidoptera: Bombycidae). Biota, v.12, n.2, p.116-121, 2007.

[2] AKAI, H.; KIGUCHI, K.; MORI, K. Increased accumulation of silk protein accompanies KJH-induced prolongation of larval life in Bombyx mori L. Applied Entomology and Zoology, v.6, n.2, p.218-220, 1971.

[3] AKAI, H.; TAKABAYASHI, K.; KIUCHI, M. Induction of spinning from JH-treated larvae of Bombyx mori by ecdysteroid administration. Journal of Sericultural Science of Japan, v.57, n.4, p.341-344, 1988.

[4] CAPPELLOZZA, L.; CAPPELLOZZA, S.; SBRENNA, G. Changes in the developmental programme of Bombyx mori caused by oral treatment with fenoxycarb during the last larval instar. Sericologia, v.35, n.3, p.427-436, 1995.

[5] CAPPELLOZZA, L.; IANNE, P.; CAPPELLOZZA, S. Effect of body weight on effectiveness of the insect growth regulator (I. G. R.) fenoxycarb applied to the male and female silkworm (Bombyx mori) (Lepidoptera: Bombycidae). Sericologia, v.37, n.3, p.443-452, 1997.

[6] CHANG, E.S. Comparative endocrinology of molting and reproduction: Insects and crustaceans. Annual Review of Entomology, v.38, n.2, p.161-180, 1993.

[7] CHOWDHARY, S.K.; SEHNAL, F.; RAJ, S.K.; RAJU, P.S.; MATHU, S. Giant cocoon formation in Bombyx mori L. topically treated with juvenile hormone SJ-42-F. Sericologia, v.26, n.4, p.455-459, 1986.

[8] CHOWDHARY, S.K.; RAJU, P.S.; OGRA, R.K. Effect of JH analogues on silkworm, Bombyx mori L., growth and development of silk gland. Sericologia, v.30, n.2, p.156-165, 1990.

[9] CIFUENTES, C.C.A.; SOHN, K.W. Manual técnico de Sericicultura: cultivo de la morera y cría del gusano de seda en el trópico. Pereira: Fundo Editorial de Risaralda, 1998. 438p.

[10] DEDOS, S.G.; FUGO, H. Disturbance of adult eclosion by fenoxycarb in the silkworm, Bombyx mori. Journal of Insect Physiology, v.45, n.2, p.257-264, 1999.

[11] EWER, J.; GAMMIE, S.C.; TRUMAN, J.W. Control of insect ecdysis by a positive-feedback endocrine system: role of eclosion hormone and ecdysis triggering hormone. Journal of Experimental Biology, v.200, n.5, p.869-881, 1997.

[12] GAABOUB, I.A.; EL-HELALY, M.S.; MOSTAFA, S.M. Food utilization, rate of larval growth, and fecundity of Bombyx mori L. (Lepidoptera: Bombycidae) fed mulberry leaves treated with methoprene, triprene, and diflubenzuron. Journal of Economical Entomology, v.78, n.6, p.1182-1186, 1985.

[13] GANGWAR, S.K. Effect of juvenile hormone mimic R394 on silkworm (Bombyx mori L.) growth and development of silk gland. Journal of Agricultural and Biological Science, v.4, n.6, p.65-67, 2009.

[14] ISHAAYA, K.; ASCHER, K.R.S. Effect of diflubenzuron on growth and carbohydrate hydrolases of Tribolium castaneum. Phytoparasitica, v.5, n.1, p.149-158, 1977.

[15] ISHAAYA, I.; HOROWITZ, R. Pyriproxyfen, a novel insect growth regulator for controlling whiteflies: mechanisms and resistance management. Pesticide Science, v.43, n.1, p.227-232, 1995.

[16] KAJIURA, Z.; KADONO-OKUDA, K.; YAMASHITA, O. Induction of dauer larvae by a juvenile hormone analogue and their response to ecdysteroids in the silkworm, Bombyx mori. Journal of Sericultural Science of Japan, v.56, n.5, p.398-406, 1987.

[17] KAJIURA, Z.; YAMASHITA, O. Super growth of silkglands in the dauer larvae of the silkworm, Bombyx mori, induced by a juvenile hormone analogue. Journal of Sericultural Science of Japan, v.58, n.1, p.39-46, 1989.

[18] KAMIMURA, M. Effects of a juvenile hormone analogue, fenoxycarb, on larval growth of the silkworm, Bombyx mori (Lepidoptera: Bombycidae). Applied Entomology and Zoology, v.30, n.3, p.487-489, 1995.

[19] KING, D.S.; SIDDALL, J. Conversion of α-ecdysone to β-ecdysone by crustaceans and insects. Nature, v.221, n.5, p.955-956, 1969.

[20] KOTIKAL, Y.; DEVAIAH, M.C. Juvenile hormones in sericulture. Indian Silk, n.25, p.19-20, 1986.

[21] KURATA, K.; DAILLIE, J. Effect of exogenous juvenoid on the growth of the silkgland and the synthesis of nucleic acid and silk protein by the silkgland of Bombyx mori. Bulletin of Sericulture Experimental Station of Japan, v.27, n.4, p.507-530, 1978.

[22] LEONARDI, M.G.; CAPPELLOZZA, S.; IANNE, P.; CAPPELLOZZA, L.; PARENTI, P.; GIORDANA, B. Effects of the topical application of an insect growth regulator (fenoxycarb) on some physiological parameters in the fifth instar larvae of the silkworm Bombyx mori. Compendium of Biochemistry and Physiology, v.113B, n.2, p.361-365, 1996.

[23] MAMATHA, D.M.; KANJII, V.K.; COHLY, H.H.P.; RAO, M.R. Juvenile hormone analogues, methoprene and fenoxycarb dose-dependently enhance certain enzyme activities in the silkworm Bombyx mori L. Environmental Research and Public Health, v.5, n.2, p.120-124, 2008.

[24] McNABB, S.L.; TRUMAN, J.W. Light and peptidergic eclosion hormone neurons stimulate a rapid eclosion response that masks circadian emergence in Drosophila. Journal of Experimental Biology, v.211, p.2263-2274, 2008.

[25] MENEGUIM, A.M.; LOVATO, L.; SILVA, R.Z. da; YAMAOKA, R.S.; NAGASHIMA, G.T.; PASINI, A. Influência de cultivares de amoreira Morus spp. sobre a produção e qualidade de casulos de bicho-da-seda, Bombyx mori L. (Lepidoptera: Bombycidae). Neotropical Entomology, v.36, n.5, p.670-674, 2007.

[26] MIRANDA, J.E.; BORTOLI, S.A.; TAKAHASHI, R. Development and silk production by silkworm larvae after topical application of methoprene. Scientia Agricola, v.59, n.3, p.585-588, 2002a.

[27] MIRANDA, J.E.; BORTOLI, S.A.; TAKAHASHI, R.; SILVA, A.F. Efeito de três análogos do hormônio juvenil aplicados em bicho-da-seda, Bombyx mori L. (Lepidoptera: Bombycidae). Neotropical Entomology, v.31, n.1, p.19-25, 2002b.

[28] MIRANDA, J.E.; BORTOLI, S.A.; TAKAHASHI, R.; SILVA, A.F. Índices nutricionais de bicho-da-seda (Bombyx mori L.) tratados com análogos do hormônio juvenil. Revista Científica Rural, v.8, n.1, p.32-38, 2003.

[29] RADHAKRISHNA, P.G.; DELVI, M.R. Effect of organophosphorous insecticides on food utilization in different races of Bombyx mori (Lepidoptera: Bombycidae). Sericologia, v.32, n.1, p.71-79, 1992.

[30] RIDDIFORD, L.M.; CHERBAS, P.; TRUMAN, J.W. Ecdysone receptors and their biological actions. Vitamins and Hormones, v.60, n.1, p.1-73, 2000.

[31] SAKURAI, S.; OKUDA, M; OHTAKI, T. Juvenile hormone inhibits ecdysone secretion and responsiveness to prothoracic glands of Bombyx mori. Genetic Comparative Endocrinology, v.75, n.2, p.222-230, 1989.

[32] SARANGI, S.K. Effect of juvenile hormone analogue on the silk gland of the silkworm, Bombyx mori L. Sericologia, v.28, n.4, p.553-557, 1988.

[33] SCHNEIDERMAN, H.A.; GILBERT, L.I. Control of growth and development in insects. Science, n.143, p.325-333, 1964.

[34] SCRIBER, J.M.; SLANSKY JUNIOR, F. The nutritional ecology of immature insects. Annual Review of Entomology, v.26, n.1, p.183-211, 1981.

[35] STAAL, G. Insect control with growth regulators interfering with the endocrine system. Entomologia Experimentalis et Applicata, v.31, n.1, p.15-23, 1982.

[36] SUBBA RAO, G.; DAS, S.K.; NANDI, S.; ROMPOMU R.; SEN, S.K. A study on the effect of a juvenile hormone analogue on the bivoltine silkworm, B. mori L. Sericologia, v.28, n.4, p.543-564, 1988.

[37] TAKAHASHI, R.; TAKAHASHI, K.M.; TAKAHASHI, L.S. Sericicultura: uma promissora exploração agropecuária Jaboticabal: Editora Funep, 2001. 126p.

[38] TSUKADA, M.; KIMURA, K.; SHIBUKAWA, A.; SHIMAZAKI, A.; AKAI, H. Effect of treatment with juvenile hormone analogue and anti-juvenoid on the size of the cocoon filament. Journal of Sericultural Science of Japan, v.56, n.3, p.231-234, 1987.

[39] WATANABE, J.K.; YAMAOKA, R.S.; BARONI, S.A. Cadeia produtiva da seda: diagnósticos e demandas atuais Londrina: IAPAR, 2000. 129p. (IAPAR, Documentos, 22).

[40] UNNI, B.G.; BHATTACHARJEE, M.; GOSWANI, A.M.; CHUTIA, S.B.; DAS, S.; RAJKHOWA, G.; KAKOTY, Y. Role of insect neuropeptides and juvenile hormones in silk protein biosynthesis. Annals of Neurosciences, v.15, n.4, p.112-117, 2008.

Brasil

Brasil

Desenvolvimento e produção de seda do Bombyx mori L. exposto a análogos do hormônio juvenil

Development and silk production of the silkworm Bombyx mori L. exposed to juvenile hormone analogues

Resumos

Datas de Publicação

Histórico