Cell damage in <i>Danio rerio</i> erythrocytes subjected to anthropized water

Several factors can lead to alterations of water quality in aquatic environments, leading to numerous consequences for the organisms that inhabit it. The water bodies located in the northwest region of Rio Grande do Sul periodically receive pollutants that may have genotoxic, cytotoxic and mutagenic properties, which affect the genetic integrity of organisms living there. This research analyzed the cytological responses of Danio rerio exposed to anthropized fresh water from the Ijuí River by the micronucleus method as well as by observing nuclear abnormalities in their erythrocytes. The data collected demonstrated higher values of erythrocytes with NA than with MN, suggesting that the cells are efficient in the repair mechanisms required when facing genotoxic substances.

Keywords:
cytological responses; micronuclei; nuclear abnormalities

Authorship

Manoel Francisco Mendes Lassen

Programa de Pós-Graduação em Ambiente e Tecnologias Sustentáveis (PPGATS). Universidade Federal da Fronteira Sul (UFFS), Rua Major Antônio Cardoso, n° 590, CEP: 97900-000, Cerro Largo, RS, Brazil. E-mail: manoellassen@gmail.com, roberta.klein@uffs.edu.br, fernando.borba@uffs.edu.br, suzymeire.baroni@uffs.edu.br Universidade Federal da Fronteira SulBrazilCerro Largo, RS, BrazilPrograma de Pós-Graduação em Ambiente e Tecnologias Sustentáveis (PPGATS). Universidade Federal da Fronteira Sul (UFFS), Rua Major Antônio Cardoso, n° 590, CEP: 97900-000, Cerro Largo, RS, Brazil. E-mail: manoellassen@gmail.com, roberta.klein@uffs.edu.br, fernando.borba@uffs.edu.br, suzymeire.baroni@uffs.edu.br

http://orcid.org/0000-0002-9104-3932

Jefferson Frohlich

Departamento de Ciências Biológicas. Universidade Federal da Fronteira Sul (UFFS), Rua Major Antônio Cardoso, n° 590, CEP: 97900-000, Cerro Largo, RS, Brazil. E-mail: frohlichjeffer@gmail.comUniversidade Federal da Fronteira SulBrazilCerro Largo, RS, BrazilDepartamento de Ciências Biológicas. Universidade Federal da Fronteira Sul (UFFS), Rua Major Antônio Cardoso, n° 590, CEP: 97900-000, Cerro Largo, RS, Brazil. E-mail: frohlichjeffer@gmail.com

http://orcid.org/0000-0002-0853-4774

Rodrigo Patera Barcelos

Programa de Pós-Graduação em Biologia Comparada. Universidade Estadual de Maringá (UEM), Avenida Colombo, n° 5790, CEP: 87020-900, Maringá, PR, Brazil. E-mail: rodrigo.barcelos@uffs.edu.brUniversidade Estadual de MaringáBrazilMaringá, PR, BrazilPrograma de Pós-Graduação em Biologia Comparada. Universidade Estadual de Maringá (UEM), Avenida Colombo, n° 5790, CEP: 87020-900, Maringá, PR, Brazil. E-mail: rodrigo.barcelos@uffs.edu.br

http://orcid.org/0000-0001-7134-647X

Roberta Daniele Klein

http://orcid.org/0000-0002-3293-2916

Fernando Henrique Borba

http://orcid.org/0000-0001-5019-020X

Suzymeire Baroni ^**Corresponding author: Suzymeire Baroni, e-mail: suzymeire.baroni@uffs.edu.br

http://orcid.org/0000-0002-0586-3978

^*Corresponding author: Suzymeire Baroni, e-mail: suzymeire.baroni@uffs.edu.br

SCIMAGO INSTITUTIONS RANKINGS

Figures | Tables

Figures (3)
Tables (2)

Thumbnail

Figure 1.
Location of the sampling points.

Thumbnail

Figure 2.
Absolute frequencies of micronuclei observed in 3,000 zebrafish erythrocytes (per aquarium), kept in control and anthropized fresh water. Values of MN and NA analyzed in 10 individuals of each test.

Thumbnail

Figure 3.
Images of the abnormalities found in blood erythrocytes of Danio rerio subjected to water collected from the four points on the Ijuí River.

Thumbnail

Table 1.
Coordinates of the water collection points on the Ijuí River with characteristics of their surrounding environments.

Thumbnail

Table 2.
Physicochemical parameters of the aquarium water at the time of zebrafish euthanasia.

Figure 1. Location of the sampling points.

Source: Own construction, 2019.

Figure 2. Absolute frequencies of micronuclei observed in 3,000 zebrafish erythrocytes (per aquarium), kept in control and anthropized fresh water. Values of MN and NA analyzed in 10 individuals of each test.

Source: Own construction, 2019.

Figure 3. Images of the abnormalities found in blood erythrocytes of Danio rerio subjected to water collected from the four points on the Ijuí River.

Caption: (A) Normal erythrocyte; (B) 1- Notched; 2 - Karyorrhexis; (C) 1 - Chromosome detachment - Micronuclei; (D) 1 - Vacuolated; 2 - Karyorrhexis; (E) 1 - Lobbed; (F) 1 - Lobbed; 2 - Blebbed; (G) 1 - Notched; 2 - Blebbed; (H) 1 - Chromosomal detachment - Micronuclei; 2 - Blebbed.Source: Own construction, 2019.

Table 1. Coordinates of the water collection points on the Ijuí River with characteristics of their surrounding environments.

Point (P)	Coordinates	Location Characteristics
P1	28°18'50.74" S 54°18'38.39" W	Absence of riparian forest and the reception of industrial and urban effluents.
P2	28°11'53.20" S 54°41'25.47" W	Absence of riparian forest, close to swine and livestock areas in addition to extensive agricultural areas upstream.
P3	28°11'19.26" S 54°43'58.56" W	Absence of riparian forest, with swine, livestock and agriculture areas. This point is located at the São José Hydroelectric Dam - Ijuí Energia S.A,and is therefore characterized as a lentic ecosystem.
P4	27° 58'0.62" S 55°19'57.82" W	Absence of riparian forest and with great livestock activity. This point is located at the mouth of the Ijuí River, which flows into the Uruguay River.

Table 2. Physicochemical parameters of the aquarium water at the time of zebrafish euthanasia.

Parameter	Control	Point 1	Point 2	Point 3	Point 4
PH	8.42	8.2	7.74	7.9	7.3
Conductivity (µS)	282	230	167.2	284	140.2
Dissolved O2 (mg/L)	9.9	9.7	5.1	11.3	12.8
H₂O Temperature (°C)	22.5	22.5	27.4	24.1	22
Ambient	24	24	28	20	24
Temperature (°C)

Source: Own construction, 2019.

How to cite

copy

Instituto de Pesquisas Ambientais em Bacias Hidrográficas Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi), Estrada Mun. Dr. José Luis Cembranelli, 5000, Taubaté, SP, Brasil, CEP 12081-010 - Taubaté - SP - Brazil
E-mail: ambi.agua@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

Versão para download de PDF

PDF

Inglês

Versões e tradução automática

Versão original do texto

English

Tradução automática

Google Translator
Microsoft Translator

Como citar

RIS

BIBTEX

Outros formatos de citação e exportação:

SciELO - Scientific Electronic Library Online
Rua Dr. Diogo de Faria, 1087 – 9º andar – Vila Clementino 04037-003 São Paulo/SP - Brasil
E-mail: scielo@scielo.org

Leia a Declaração de Acesso Aberto

Métricas

Cell damage in <i>Danio rerio</i> erythrocytes subjected to anthropized water

PlumX

Mensagem

[1] ^*Corresponding author: Suzymeire Baroni, e-mail: suzymeire.baroni@uffs.edu.br