Application of a voltammetric enzymatic biosensor based on crude extract of <i>Marasmiellus colocasiae</i> for the detection of phenolic compounds in drinking water

Batista, Érica Aparecida; Garcia, Luane Ferreira; Albuquerque, Antonio João Carvalho de; Ballaminut, Nara; Scalize, Paulo Sérgio; Gil, Eric Souza

doi:10.4136/ambi-agua.2610

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ARTICLES • Rev. Ambient. Água 15 (6) • 2020 • https://doi.org/10.4136/ambi-agua.2610 copy

Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water

Aplicação de um biosensor enzimático voltamétrico à base de extrato bruto de Marasmiellus colocasiae para a detecção de compostos fenólicos em água potável

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

The chemical and pharmaceutical industries are the main generators of residues, such as phenolic microcontaminants, including catechol, resorcinol, p-nitrophenol and 4-chlorophenol. Therefore, this work aims to identify these microcontaminants through an emerging contaminant biosensor by means of an enzymatic biosensor constructed with an enzymatic extract from the fungus Marasmiellus colocasiae. Based on the differential pulse voltammetry electrochemical technique, the biosensor was used to analyze the patterns of catechol, resorcinol, p-nitrophenol and 4-chlorophenol. The analysis of a sample prepared with these standards in water from the public supply network was also carried out. As a result, it was possible to verify that the biosensor developed in this study is more sensitive than conventional methods and has a greater affinity for catechol. In the sample prepared with the standards, it was possible to qualitatively identify the presence of 4-chlorophenol, resorcinol and catechol. The proposed biosensor was sensitive and has potential for application in the analysis of microcontaminants in the environment with the detection limit = 0.17 µmol L^-1, and the quantification limit = 0.52 µmol L^-1.

Keywords:
electrochemical biosensor; micropollutants; polyphenol oxidases; potable water

Instituto de Pesquisas Ambientais em Bacias Hidrográficas Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi), Estrada Mun. Dr. José Luis Cembranelli, 5000, Taubaté, SP, Brasil, CEP 12081-010 - Taubaté - SP - Brazil
E-mail: ambi.agua@gmail.com

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[1] ^*Corresponding author: Paulo Sérgio Scalize, e-mail: pscalize.ufg@gmail.com

Enzyme source	Enzymatic activity	Method	Linear range (μmol L^-1)	Limit of detection (μmol L^-1)	Reference
M. colocasiae	1286.20 U L^-1	DPV	50 - 300	0.17	This work
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Purchased from Sigma	1050 U mg^-1	Cyclic voltammetry	1.0-100	0.01	(*Chen et al., 2013*CHEN, H. et al. A new catechol biosensor immobilized polyphenol oxidase by combining electropolymerization and cross-linking process. International Journal of Polymeric Materials and Polymeric Biomaterials, v. 62, n. 12, p. 620-626, 2013. https://doi.org/10.1080/00914037.2012.761629 https://doi.org/10.1080/00914037.2012.76... )