The Tradescantia pallida var. purpurea active bioassay for water monitoring: evaluating and comparing methodological conditions

O bioensaio ativo com Tradescantia pallida var. purpurea para monitoramento de água: avaliação e comparação de condições metodológicas

Abstracts

Tradescantia pallida var. purpurea cuttings with flower buds are utilized in bioassays to diagnose genotoxic effects of water. The literature describes different substances used to adapt and recover the cuttings before and after exposure to water samples and also describes the effects of different exposure times. This study evaluated and compared the micronuclei (MCN) frequencies in T. pallida when cuttings with flower buds were submitted to different methodological conditions. The bioassay was then applied bimonthly during seven months to assess the genotoxic potential of a site located on the Sinos River in Campo Bom, Rio Grande do Sul, Brazil. Micronuclei frequencies in buds of cuttings adapted and recovered in distilled water and in Hoagland solution were 3.0 and 2.9, respectively, for cuttings exposed to river water, and 1.19 and 1.23 in controls. No significant differences among MCN frequencies were observed when cuttings were exposed for 8, 24 or 32 hours to river water (from 3.07 to 4.73) and in controls (from 1.13 to 2.00) in all samplings during a year. Adaptation and recovery of cuttings in distilled water or Hoagland solution and exposure for different times did not influence the response of T. pallida, indicating that all the conditions tested are viable for biomonitoring of water genotoxicity. Water samples from the Sinos River presented genotoxicity during the period monitored, evidenced by the MCN frequencies recorded which were significantly higher than the frequencies of the controls.

water bodies; genotoxic risk; micronuclei

Ramos de Tradescantia pallida var. purpurea contendo botões florais são utilizados em bioensaios para diagnosticar efeitos genotóxicos de água. Diferentes substâncias para adaptar e recuperar os ramos antes e após a exposição a amostras de água e diferentes tempos de exposição são relatados na literatura. O objetivo do estudo foi avaliar e comparar as frequências de micronúcleos (MCN) em T. pallida quando ramos com botões florais foram submetidos a diferentes condições metodológicas. O bioensaio foi então aplicado bimensalmente durante sete meses para avaliar o potencial genotóxico de um sítio do Rio dos Sinos em Campo Bom, Rio Grande do Sul, Brasil. As frequências de MCN em botões de ramos adaptados e recuperados em água destilada e em solução de Hoagland foram de 3,0 e 2,9, respectivamente, quando ramos foram expostos a água do rio e de 1,19 e 1,23 nos controles. Não foram observadas diferenças significantes entre frequências de MCN em ramos expostos por 8, 24 ou 32 h a água do rio (de 3,07 a 4,73) e nos controles (de 1,13 a 2,00) em todas as amostragens ao longo do ano. Adaptação e recuperação de ramos em água destilada ou solução de Hoagland e exposição durante diferentes tempos não influenciaram a resposta de T. pallida de formas diferentes, indicando que todas as condições testadas são viáveis para biomonitoramento de genotoxicidade de água. Amostras de água do Rio dos Sinos apresentaram genotoxicidade ao longo do período monitorado, evidenciada pelas frequências de MCN registradas, que foram significativamente superiores às dos controles.

corpos d'água; risco genotóxico; micronúcleos

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Publication Dates

• Publication in this collection
  17 Sept 2014
• Date of issue
  Sept 2014

History

• Received
  02 June 2014
• Accepted
  24 July 2014