Acessibilidade / Reportar erro

Characterization of Taenia crassiceps cysticerci and their application to diagnosis of the human cysticercosis

THESES

Characterization of Taenia crassiceps cysticerci and their application to diagnosis of the human cysticercosis (Abstract)* * Caracterização de antígenos de cisticercos e sua aplicação para o diagnóstico da cisticercose humana (Rsumo). Tese (Doutorado), Universidade de São Paulo (Área: Análises clínicas). Orientador: Adelaide José Vaz. ** Adress: Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmaceuticas da USP. Avenida Prof. Lineu Pretes 580 bloco 17, 05508-900 São Paulo SP, Brasil. E-mail: ajvaz@usp.br . Thesis . São Paulo, 2004

Noeli Maria Espíndola** * Caracterização de antígenos de cisticercos e sua aplicação para o diagnóstico da cisticercose humana (Rsumo). Tese (Doutorado), Universidade de São Paulo (Área: Análises clínicas). Orientador: Adelaide José Vaz. ** Adress: Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmaceuticas da USP. Avenida Prof. Lineu Pretes 580 bloco 17, 05508-900 São Paulo SP, Brasil. E-mail: ajvaz@usp.br

Taenia crassiceps cysticerci were kept in vitro during 144h. Excretory / secretory (ES) antigens (peptides of <30kDa) were identified in 24 (ES24) and 48h (ES48). ES peptides (30-, 18- and 14-12-kDa) were recognized by polyclonal antibodies in sera from rabbits immunized with Taenia crassiceps (Tcra) and Taenia solium (Tso) antigens, and also by antibodies in samples of serum and cerebrospinal fluid (CSF) from patients with neurocysticercosis (NC).

Monoclonal antibodies (MoAb) were prepared against ES-Tcra, Tcra vesicular fluid (VF-Tcra), Tso vesicular fluid (VF-Tso), Tso total (T-Tso) and scolex (E-Tso) antigens.

Twenty-four MoAb were obtained and they were used for the characterization of Tcra and Tso antigens, and for the antigen detection in samples of patients with NC. Nine MoAb (1 anti-ES-Tra, 1 anti-VF-Tcra, 3 anti-VF-Tso and 4 anti-T-Tso) reacted with peptides in the Tcra (18- and 14-12-kDa) Tso (14-12kDa) antigens. These low-molecular-weight peptides have been considered the immunodominant and specific antigen for the diagnosis of NC.

The MoAb maintained their reactivity towards VF-Tcra antigen treated with heat and alkaline borohydride and showed reduction after trichloroacetic acid and periodate oxidation treatments. These results indicate that the 18- and 14-12-kDa fractions are glycoproteins.

Peptides of <20kDa in VF-Tcra antigen were purified by anti-Tcra MoAb and were used to detect antibodies in serum and CSF from patients with NC. Anti-Tcra and anti-Tso MoAb were used to detect antibodies by a competitive assay, and anti-Tcra MoAb to detect antigens in serum and CSF from patients with NC. The results showed a better performance than the use of brute antigens or polyclonal antibodies.

Key words:Taenia crassiceps antigens, neurocysticercosis diagnosis, cerebrospinal fluid.

  • *
    Caracterização de antígenos de cisticercos e sua aplicação para o diagnóstico da cisticercose humana (Rsumo). Tese (Doutorado), Universidade de São Paulo (Área: Análises clínicas). Orientador: Adelaide José Vaz.
    **
    Adress: Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmaceuticas da USP. Avenida Prof. Lineu Pretes 580 bloco 17, 05508-900 São Paulo SP, Brasil. E-mail:
  • Publication Dates

    • Publication in this collection
      23 June 2004
    • Date of issue
      June 2004
    Academia Brasileira de Neurologia - ABNEURO R. Vergueiro, 1353 sl.1404 - Ed. Top Towers Offices Torre Norte, 04101-000 São Paulo SP Brazil, Tel.: +55 11 5084-9463 | +55 11 5083-3876 - São Paulo - SP - Brazil
    E-mail: revista.arquivos@abneuro.org