Human neurocysticercosis: IgE in cerebrospinal fluid

Melo, Carmen Silvia de; Vaz, Adelaide José; Nakamura, Paulo Mutuko; Silva, Marcos Vinicius da; Machado, Aluízio de Barros Barreto

doi:10.1590/S0004-282X1997000100002

Abstracts

The detection of IgE is technically difficult because of its reduced concentrations in serum, and even lower concentrations in cerebrospinal fluid (CSF). In the present investigation we studied 86 CSF samples using an immunoenzymatic method with an anti-IgE-alkaline phosphatase conjugate and a fluorigenic substrate. The samples were from three groups: A) 29 patients with neurocysticercosis (NC), B) 36 patients with different neurologic disorders (neurosyphilis, neurotuberculosis, meningitis, tumors, hemorrhage) and C) 21 discharged individuals who had been hospitalized for bacterial meningitis. The results obtained were: A) 0.05 to 3.00 IU/ml (0.76±0.79), B) 0.00 to 1.50 IU/ml (0.23±0.34) and C) 0.05 to 1.25 IU/ml (0.34±0.34). The present results suggest that IgE appears to play a role in the pathogeny of NC and that efforts should be made to standardize a test for the detection of specific IgE antibodies.

neurocysticercosis; cerebrospinal fluid; IgE; immunoenzymatic assay

A detecção de IgE apresenta dificuldades técnicas pela reduzida concentração que se encontra no LCR e no soro. Utilizando método imunoenzimático com conjugado anti-IgE-fosfatase alcalina e substrato fluorigênico, foram estudadas 86 amostras de LCR de três grupos: A) 29 pacientes com NC, B) 36 pacientes com afecções neurológicas diversas (neurossífilis, neurotuberculose, meningites, tumores, hemorragias) e C) 21 indivíduos de pós-alta médica de meningites bacterianas. Os resultados obtidos foram: A) 0,05 a 3,00 Ul/ml (0,76±0,79), B) 0,00 a 1,50 Ul/ml (0,23±0,34) e C) 0,05 a 1,25 Ul/ml (0,34±0,34). Os resultados obtidos sugerem que a IgE parece ter papel na patogenia da NC e esforços devem ser feitos para padronização de teste para detecção de anticorpos IgE específicos.

neurocisticercose; líquido cefalorraquidiano; IgE; teste imunoenzimático

Human neurocysticercosis: IgE in cerebrospinal fluid

Neurocisticercose humana: IgE no líquido cefalorraquiano

Carmen Silvia de Melo^I; Adelaide José Vaz^II; Paulo Mutuko Nakamura^I; Marcos Vinicius da Silva^III; Aluízio de Barros Barreto Machado^IV

^IResearcher, Adolfo Lutz Institute, São Paulo

^IIAssistant Professor, Faculty of Pharmaceutical Sciences, University of São Paulo

^IIIPhysician, Emilio Ribas Infectology Institute, São Paulo

^IVPhysician, University Hospital, Faculty of Medicine, University of São Paulo, São Paulo

ABSTRACT

The detection of IgE is technically difficult because of its reduced concentrations in serum, and even lower concentrations in cerebrospinal fluid (CSF). In the present investigation we studied 86 CSF samples using an immunoenzymatic method with an anti-IgE-alkaline phosphatase conjugate and a fluorigenic substrate. The samples were from three groups: A) 29 patients with neurocysticercosis (NC), B) 36 patients with different neurologic disorders (neurosyphilis, neurotuberculosis, meningitis, tumors, hemorrhage) and C) 21 discharged individuals who had been hospitalized for bacterial meningitis. The results obtained were: A) 0.05 to 3.00 IU/ml (0.76±0.79), B) 0.00 to 1.50 IU/ml (0.23±0.34) and C) 0.05 to 1.25 IU/ml (0.34±0.34). The present results suggest that IgE appears to play a role in the pathogeny of NC and that efforts should be made to standardize a test for the detection of specific IgE antibodies.

Key words: neurocysticercosis, cerebrospinal fluid, IgE, immunoenzymatic assay.

RESUMO

A detecção de IgE apresenta dificuldades técnicas pela reduzida concentração que se encontra no LCR e no soro. Utilizando método imunoenzimático com conjugado anti-IgE-fosfatase alcalina e substrato fluorigênico, foram estudadas 86 amostras de LCR de três grupos: A) 29 pacientes com NC, B) 36 pacientes com afecções neurológicas diversas (neurossífilis, neurotuberculose, meningites, tumores, hemorragias) e C) 21 indivíduos de pós-alta médica de meningites bacterianas. Os resultados obtidos foram: A) 0,05 a 3,00 Ul/ml (0,76±0,79), B) 0,00 a 1,50 Ul/ml (0,23±0,34) e C) 0,05 a 1,25 Ul/ml (0,34±0,34). Os resultados obtidos sugerem que a IgE parece ter papel na patogenia da NC e esforços devem ser feitos para padronização de teste para detecção de anticorpos IgE específicos.

Palavras-chave: neurocisticercose, líquido cefalorraquidiano, IgE, teste imunoenzimático.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Aceite: 15-outubro-1996

Adolfo Lutz Institute, Serology Section, São Paulo, SP, Brazil.

Dra. Adelaide José Vaz - Avenida Professor Lineu Prestes 580, Bloco 17 - 05508-900 São Paulo SP - Brasil.

1. Anderson MJD, Griffin JFT. Taenia crassiceps in rats: differences in susceptibility to infection and development of immunocompetence in relation to age and host strain. Int J Parasitol 1979;9:229-233.
2. Espinoza B, Ruiz-Palacios G, Tovar A, Sandoval MA, Plancarte A, Flisser A. Characterization by enzyme-linked immunosorbent assay of the humoral response in patients with neurocysticercosis and its application in immunodiagnosis. J Clin Microbiol 1986;24:536-541.
3. Flisser A, Woodhouse E, Larralde C. Human cysticercosis: antigens, antibodies and non-responders. Clin Exp Immunol 1980;39:27-37.
4. Goldberg AS, Heiner DC, Firemark HM, Goldberg MA. Cerebrospinal fluid IgE and the diagnosis of cerebral cysticercosis. Bull Los Angeles Neurol Soc 1981,46:21-25.
5. Gorodezky C, Diaz ML, Escobar-Gutierrez A, Flisser A. IgE concentration in sera of patients with neurocysticercosis. Arch Invest Med 1987;18:225-227.
6. Livramento JA. Síndrome do líquido cefalorraqueano na neurocisticercose. Arq Neuropsiquiatr 1987;45:261-275.
7. Livramento JA, Machado LR, Spina-França A. Immunobiology of neurocysticercosis. In Fejerman N, Chamoles NA (eds). New trends in pediatric neurology. New York: Elsevier, 1993:307-312.
8. Molinari JL, Tato P, Lara-Aguilera R, White AC Jr. Effects of serum from neurocysticercosis patients on the structure and viability of Taenia solium oncospheres. J Parasitol 1993,79:124-127.
9. Ottesen EA, Poindexter RW, Hussain R. Detection, quantitation and specificity of antiparasite IgE antibodies in human schistosomiasis mansoni. Am J Trop Med Hyg 1981 ;30:1228-1237.
10. Parkhouse RME, Harrison OS. Antigens of parasite helminthes and diagnosis, protection and pathology. Parasitology 1989; 99(Suppl.):S5-S19.
11. Pialarissi CSM, Vaz, AJ, Souza AMC, Nakamura PM, Camargo ED, Silva MV.Ueda M. Estudo comparativo de testes sorológicos no diagnóstico imunológico da neurocisticercose. Rev Inst Med Trop S Paulo 1987;29:367-373.
12. Polmar SH, Waldmann TA, Terry WD. A comparison of three radioimmunoassay techniques for the measurement of serum IgE. J Immunol 1973;110:1253-1261.
13. Short JA, Heiner DC, Hsiao RL, Andersen FL. Immunoglobulin E and G4 antibodies in cysticercosis. J Clin Microbiol 1990; 28:1635-1639.
14 . Spina-França A. Patogenia das infecções do SNC e LCR: análise crítica da contribuição diagnostica. Rev Paul Med 1989;107:169-174.
15. Spina-França A, Kleine TO, Livramento J A, Machado LR. IgE determination in cerebrospinal fluid and serum of patients with neurocysticercosis by a modified enzyme immunoassay. Protides in Biological Fluids 1986;34:657-660.
16. Urquiza HD. Algunas consideraciones sobre la determination de immunoglobulina E en el suero del cordon y la prevention de enfermedades alérgicas. Rev Cub Med Gen Integral 1988;4:28-36.
17. Vaz AJ, Ferreira AW. Imunodiagnóstico da neurocisticercose: teste imunoenzimático com antigenos quimicamente ligados a suportes para pesquisa de anticorpos em soro e líquido cefalorraquiano. Rev Inst Med Trop S Paulo 1988;30:1-10.
18. Vaz AJ. Cysticercus longicollis: caracterização antigênica e desenvolvimento de testes imunológicos para pesquisa de anticorpos em líquido cefalorraquiano no imunodiagnóstico da neurocisticercose humana. Tese de Doutorado, Instituto de Ciências Biomédicas da Universidade de São Paulo. São Paulo, 1993.
19. Zakroff SGH, Beck L, Platzer EG, Spiegelberg HL. The IgE and IgG subclass responses of mice to four helminth parasites. Cell Immunol 1989;119:193-201.

Publication Dates

Publication in this collection
10 Nov 2010
Date of issue
1997

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Anderson MJD, Griffin JFT. Taenia crassiceps in rats: differences in susceptibility to infection and development of immunocompetence in relation to age and host strain. Int J Parasitol 1979;9:229-233.

[2] 2. Espinoza B, Ruiz-Palacios G, Tovar A, Sandoval MA, Plancarte A, Flisser A. Characterization by enzyme-linked immunosorbent assay of the humoral response in patients with neurocysticercosis and its application in immunodiagnosis. J Clin Microbiol 1986;24:536-541.

[3] 3. Flisser A, Woodhouse E, Larralde C. Human cysticercosis: antigens, antibodies and non-responders. Clin Exp Immunol 1980;39:27-37.

[4] 4. Goldberg AS, Heiner DC, Firemark HM, Goldberg MA. Cerebrospinal fluid IgE and the diagnosis of cerebral cysticercosis. Bull Los Angeles Neurol Soc 1981,46:21-25.

[5] 5. Gorodezky C, Diaz ML, Escobar-Gutierrez A, Flisser A. IgE concentration in sera of patients with neurocysticercosis. Arch Invest Med 1987;18:225-227.

[6] 6. Livramento JA. Síndrome do líquido cefalorraqueano na neurocisticercose. Arq Neuropsiquiatr 1987;45:261-275.

[7] 7. Livramento JA, Machado LR, Spina-França A. Immunobiology of neurocysticercosis. In Fejerman N, Chamoles NA (eds). New trends in pediatric neurology. New York: Elsevier, 1993:307-312.

[8] 8. Molinari JL, Tato P, Lara-Aguilera R, White AC Jr. Effects of serum from neurocysticercosis patients on the structure and viability of Taenia solium oncospheres. J Parasitol 1993,79:124-127.

[9] 9. Ottesen EA, Poindexter RW, Hussain R. Detection, quantitation and specificity of antiparasite IgE antibodies in human schistosomiasis mansoni. Am J Trop Med Hyg 1981 ;30:1228-1237.

[10] 10. Parkhouse RME, Harrison OS. Antigens of parasite helminthes and diagnosis, protection and pathology. Parasitology 1989; 99(Suppl.):S5-S19.

[11] 11. Pialarissi CSM, Vaz, AJ, Souza AMC, Nakamura PM, Camargo ED, Silva MV.Ueda M. Estudo comparativo de testes sorológicos no diagnóstico imunológico da neurocisticercose. Rev Inst Med Trop S Paulo 1987;29:367-373.

[12] 12. Polmar SH, Waldmann TA, Terry WD. A comparison of three radioimmunoassay techniques for the measurement of serum IgE. J Immunol 1973;110:1253-1261.

[13] 13. Short JA, Heiner DC, Hsiao RL, Andersen FL. Immunoglobulin E and G4 antibodies in cysticercosis. J Clin Microbiol 1990; 28:1635-1639.

[14] 14 . Spina-França A. Patogenia das infecções do SNC e LCR: análise crítica da contribuição diagnostica. Rev Paul Med 1989;107:169-174.

[15] 15. Spina-França A, Kleine TO, Livramento J A, Machado LR. IgE determination in cerebrospinal fluid and serum of patients with neurocysticercosis by a modified enzyme immunoassay. Protides in Biological Fluids 1986;34:657-660.

[16] 16. Urquiza HD. Algunas consideraciones sobre la determination de immunoglobulina E en el suero del cordon y la prevention de enfermedades alérgicas. Rev Cub Med Gen Integral 1988;4:28-36.

[17] 17. Vaz AJ, Ferreira AW. Imunodiagnóstico da neurocisticercose: teste imunoenzimático com antigenos quimicamente ligados a suportes para pesquisa de anticorpos em soro e líquido cefalorraquiano. Rev Inst Med Trop S Paulo 1988;30:1-10.

[18] 18. Vaz AJ. Cysticercus longicollis: caracterização antigênica e desenvolvimento de testes imunológicos para pesquisa de anticorpos em líquido cefalorraquiano no imunodiagnóstico da neurocisticercose humana. Tese de Doutorado, Instituto de Ciências Biomédicas da Universidade de São Paulo. São Paulo, 1993.

[19] 19. Zakroff SGH, Beck L, Platzer EG, Spiegelberg HL. The IgE and IgG subclass responses of mice to four helminth parasites. Cell Immunol 1989;119:193-201.