Proteinas do liquido cefalorraqueano: III. Valores normais das imuneglobulinas G, A e M (variações ligadas a cor, sexo e idade)

Vermes, Lucia M. Singer

doi:10.1590/S0004-282X1983000100003

Resumos

É apresentada inicialmente revisão de literatura a respeito dos resultados obtidos na determinação das taxas de imuneglobulinas do LCR normal, mediante diferentes métodos, assim como da influência dos fatores cor, sexo e idade, nestes valores. Mediante imunodifusão radial foram determinados os níveis de IgG, IgA e IgM do LCR normal de 115, 78 e 45 pessoas, respectivamente. Todas as amostras de LCR foram obtidas por punção sub-occipital (LCR-SO). Os resultados destas determinações permitiram concluir que: a) as taxas médias de IgG, IgA e IgM do LCR de pretos ou pardos não diferem daquelas de brancos; b) não existem variações ligadas ao sexo, nem à idade, em crianças com 1 a 11 anos, nas taxas das imuneglobulinas estudadas, no LCR; c) os níveis fisiológicos em crianças com 1 a 11 anos, para IgG variam entre 0,21 e 2,93 mg/100 ml; os teores absolutos médios de IgG de crianças são inferiores àqueles encontrados em adultos; os níveis percentuais médios de IgG de crianças não diferem daqueles de adultos; d) as taxas de IgA do LCR de crianças, tanto em valores absolutos como relativos, são, em média, inferiores àquelas encontradas em adultos, não ultrapassando, em condições normais, 0,15 mg/100 ml (0,7% do total protéico); e) em adultos, não se observam variações ligadas ao sexo, nem à idade, nos níveis das imuneglobulinas estudadas, no LCR-SO; as taxas normais de IgG encontradas no LCR-SO de adultos são de 0,51 a 4,00 mg/100 ml; em crianças e adultos, as taxas fisiológicas desta imuneglobulina, em termos percentuais, são de 3 a 12%; o LCR-SO normal de adultos contém IgA em concentrações de até 0,32 mg/100 ml (0,9% do total protéico); f) a IgM encontra-se no LCR-SO normal, tanto de crianças como de adultos, em níveis muito baixos, não ultrapassando 0,2 mg/100 ml (0,25% do total protéico).

The literature on quantitation of immunoglobulins in normal CSF and variations of these values related to race, sex and age was reviewed. Immunoglobulins (Ig) G, A and M of normal CSF (sub-occipital puncture) of 116, 78 and 45 patients respectively, were measured by radial immunodiffusion, in order to verify variations related to race, sex and age, as well as to establish their normal limits. The results allowed us to conclude that: a) there are no differences between races with respect to the levels of IgG, A and M; b) variations related to sex or age on the CFS immunoglobulins content in children (1 to 11 years old) are not found; c) the mean IgG (mg/100 ml) level in children is lower than in adults, although the mean IgG percentage concentration in children is not different from that found in adults; the normal range for CSF IgG in children is 0.21 to 2.93 mg/100 ml; d) the mean IgA content (mg/100 ml and % of the total protein) in children are lower than in adults and in physiological conditions do not exceed 0.15 mg/100 ml (0.7% of the total protein); e) there are no differences related to sex or age with respect to the CSF levels of immunoglobulins in adults; the normal range of IgG in adults is 0.51 to 4.00 mg/100 ml; in children and adults the normal limits for the relative values of IgG are 3 to 12%; IgA is found in the CFS of adults in a concentration up to 0.32 mg/100 ml (0.9%); f) IgM is found in both children's and adults' CSF at very low levels, not exceeding 0.2 mg/100 ml (0.25%). Comparison of the results obtained in this work with those found in some publications was carried out and is briefly discussed.

Proteinas do liquido cefalorraqueano: III. Valores normais das imuneglobulinas G, A e M (variações ligadas a cor, sexo e idade)

Cerebrospinal fluid proteins: III. Immunoglobulins G, A and M normal values (variations related to race, sex and age)

Lucia M. Singer Vermes

Professora Assistente do Departamento de Microbiologia e Imunologia do Instituto de Ciências Biomédicas da Universidade de São Paulo

RESUMO

É apresentada inicialmente revisão de literatura a respeito dos resultados obtidos na determinação das taxas de imuneglobulinas do LCR normal, mediante diferentes métodos, assim como da influência dos fatores cor, sexo e idade, nestes valores.

Mediante imunodifusão radial foram determinados os níveis de IgG, IgA e IgM do LCR normal de 115, 78 e 45 pessoas, respectivamente. Todas as amostras de LCR foram obtidas por punção sub-occipital (LCR-SO).

Os resultados destas determinações permitiram concluir que: a) as taxas médias de IgG, IgA e IgM do LCR de pretos ou pardos não diferem daquelas de brancos; b) não existem variações ligadas ao sexo, nem à idade, em crianças com 1 a 11 anos, nas taxas das imuneglobulinas estudadas, no LCR; c) os níveis fisiológicos em crianças com 1 a 11 anos, para IgG variam entre 0,21 e 2,93 mg/100 ml; os teores absolutos médios de IgG de crianças são inferiores àqueles encontrados em adultos; os níveis percentuais médios de IgG de crianças não diferem daqueles de adultos; d) as taxas de IgA do LCR de crianças, tanto em valores absolutos como relativos, são, em média, inferiores àquelas encontradas em adultos, não ultrapassando, em condições normais, 0,15 mg/100 ml (0,7% do total protéico); e) em adultos, não se observam variações ligadas ao sexo, nem à idade, nos níveis das imuneglobulinas estudadas, no LCR-SO; as taxas normais de IgG encontradas no LCR-SO de adultos são de 0,51 a 4,00 mg/100 ml; em crianças e adultos, as taxas fisiológicas desta imuneglobulina, em termos percentuais, são de 3 a 12%; o LCR-SO normal de adultos contém IgA em concentrações de até 0,32 mg/100 ml (0,9% do total protéico); f) a IgM encontra-se no LCR-SO normal, tanto de crianças como de adultos, em níveis muito baixos, não ultrapassando 0,2 mg/100 ml (0,25% do total protéico).

SUMMARY

The literature on quantitation of immunoglobulins in normal CSF and variations of these values related to race, sex and age was reviewed. Immunoglobulins (Ig) G, A and M of normal CSF (sub-occipital puncture) of 116, 78 and 45 patients respectively, were measured by radial immunodiffusion, in order to verify variations related to race, sex and age, as well as to establish their normal limits.

The results allowed us to conclude that: a) there are no differences between races with respect to the levels of IgG, A and M; b) variations related to sex or age on the CFS immunoglobulins content in children (1 to 11 years old) are not found; c) the mean IgG (mg/100 ml) level in children is lower than in adults, although the mean IgG percentage concentration in children is not different from that found in adults; the normal range for CSF IgG in children is 0.21 to 2.93 mg/100 ml; d) the mean IgA content (mg/100 ml and % of the total protein) in children are lower than in adults and in physiological conditions do not exceed 0.15 mg/100 ml (0.7% of the total protein); e) there are no differences related to sex or age with respect to the CSF levels of immunoglobulins in adults; the normal range of IgG in adults is 0.51 to 4.00 mg/100 ml; in children and adults the normal limits for the relative values of IgG are 3 to 12%; IgA is found in the CFS of adults in a concentration up to 0.32 mg/100 ml (0.9%); f) IgM is found in both children's and adults' CSF at very low levels, not exceeding 0.2 mg/100 ml (0.25%).

Comparison of the results obtained in this work with those found in some publications was carried out and is briefly discussed.

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Resumo da tese de doutoramento "Líquido Cefalorraqueano Normal: métodos de concentração, proteinograma e imuneglobulinas", defendida no Departamento de Microbiologia e Imunologia do Instituto de Ciências Biomédicas da Universidade de São Paulo.

Departamento de Microbiologia e Imunologia - Instituto de Ciências Biomédicas -Universidade de São Paulo - Caixa Postal 2921 - 01000 São Paulo, SP - Brasil

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4. BESSET, A.; DESLANDES, A. & MOURAY, H. - Dosage simultané des IgG et de l'albumine dans le liquide céphalo-rachidien. Ann. Biol. clin. 32:507, 1974.
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Datas de Publicação

Publicação nesta coleção
14 Ago 2012
Data do Fascículo
Mar 1983

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[1] 1. AHONEN, A.; MYLLYLÄ, V. V. & HOKKANEN, E. - Measurement of reference values for certain proteins in cerebrospinal fluid. Acta neurol. scand. 57:358, 1978.

[2] 2. AHONEN, A.; MYLLYLÄ, V. V. & HOKKANEN, E. - Cerebrospinal fluid protein findings in various lower back pain syndromes. Acta neurol. scand. 60:93, 1979.

[3] 3. BERNHARDT, W.; WEISNER, B. & REHPENNING, W. - Liquorbefund und Lebensalter. Trivariate Wertung der Konzentrationen von Albumin und Immunglobulin G. J. clin. Chem. clin. Biochem. 16:435, 1978.

[4] 4. BESSET, A.; DESLANDES, A. & MOURAY, H. - Dosage simultané des IgG et de l'albumine dans le liquide céphalo-rachidien. Ann. Biol. clin. 32:507, 1974.

[5] 5. BOCK, E. - Quantitation of plasma proteins in cerebrospinal fluid. Scand. J. Immunol. 2 (Suppl. 1):111, 1973.

[6] 6. BOCK, E. ;KRISTENSEN, V. & RAFAELSEN, O. J. - Proteins in serum and cerebrospinal fluid in demented patients. Acta, neurol. scand. 50:91, 1974.

[7] 7. BUCHANAM, N. & MACNAB, G. - Cerebrospinal fluid complement and immunoglobulins in meningitis and encephalitis. S. Afr. med. J. 46:1376, 1972.

[8] 8. CLAUSEN, J.; FOG, T. & EINSTEIN, E. R. - The clinical value of assaying proteins in the cerebrospinal fluid. Acta neurol. scand. 45:513, 1969.

[9] 9. DELANK, H. W. & WREDE, M. T. - Der Klinische Wert quantitativ immunochemischer Bestimmungen verschiedener Proteine im Liquor cerebrospinalis. Klin. Wsçhr. 47:1270, 1969.

[10] 10. DELPECH, B. & ROUSSEAU, P. Y. - Dosage par immuno-diffusion radiale simple des g G globulines et de la sidérophiline du liquide céphalo-rachidien normal. Rev. franc. Étud. Clin. biol. 14:694, 1969.

[11] 11. DENCKER, S. J. - Quantification of individual CSF proteins by immune precipitation in agar gel. J. Neurochem. 16:465, 1969.

[12] 12. DENCKER, S. J. - Quantification of individual proteins by immuno-diffusion. Acta neurol. scand. 46 (Suppl. 43):238, 1970.

[13] 13. EICKHOFF, K.; KASCHKA, W.; SKVARIL, L.; THEILKAES, L. & HEIPERTZ, R. - Determination of IgG subgroups in cerebrospinal fluid of multiple sclerosis patients and others. Acta neurol. scand. 60:277, 1979.

[14] 14. FAHEY, J. L. & McKELVEY, E. M. - Quantitative determination of serum immunoglobulins in antibody agar plates. J. Immunol. 94:84, 1965.

[15] 15. FIOCCA, S. - Esame del liquido cefalo-rachidiano. Ann. Sclavo. 19:844, 1977.

[16] 16- FISCHER-WILLIAMS, M. & ROBERTS, R. C. - Cerebrospinal fluid proteins and serum immunoglobulins. Arch. Neurol. (Chic). 25:526, 1971.

[17] 17. GANROT, K. & LAURELL, C. B. - Measurement of IgG and albumin content of cerebrospinal fluid, and its interpretation. Clin. Chem. 20:571, 1974.

[18] 18. GANROT-NORLIN, K. - Relative concentration of albumin and IgG in cerebrospinal fluid in health and in acute meningitis. Scand. J. infect. Dis. 10:57, 1978.

[19] 19. GLOSNER, H. & WENIG, C. - Zur Pathophysiologie der Liquorimmunglobuline. Klin. Wschr. 51:806, 1973.

[20] 20. GOODMAN, M. & VULPE, M. - A quantitative immunochemical method for determining serum and cerebrospinal fluid proteins. Wld. Neurol. 2:589, 1961.

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