Diatraea saccharalis is the principal pest of sugarcane in Brazil and is found throughout the sugarcane crop. Information about its population genetics is scarce, but population genetic analysis is of particular importance as a basis for a successful pest control program. Pest control requires a constant evaluation of genetic variability so that appropriate strategies can be employed. In this study, the structure of D. saccharalis populations in sugarcane crops was analyzed with PCR-RAPD (Polymerase Chain Reaction-Random Amplified Polymorphic DNA) markers. Samples were collected from four sugarcane fields in ParanáState and one in São PauloState. Twelve PCR-RAPD primers were used. The analysis produced 216 fragments, with a 99.54% polymorphism rate. The Shannon index averaged 0.3797 (± 0.1729), and the average value of GST for the five populations was 0.0909. These results showed that the populations are differentiated. An analysis of molecular variance showed that 92% of the polymorphism occurs within populations. The high polymorphism rate within these populations shows that these insects have the potential to develop resistance to insecticides. The control and management of D. saccharalis should be conducted cautiously and integrated using chemical and biological control.
molecular marker; polymorphism; PCR-RAPD; sugarcane pest
