Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.

Markovic, Marija; Grbic, Mihailo; Djukic, Matilda

doi:10.1590/1678-4324-2016150320

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Biological and Applied Sciences • Braz. arch. biol. technol. 59 • 2016 • https://doi.org/10.1590/1678-4324-2016150320 copy

Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.

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The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).

pineleaf pink; in vitro culture; BAP; NAA; multiplication

BAP mg/L	NAA mg/L	single node cuttings (%)	terminal buds (%)	shoot cuttings (%)
BAP mg/L	NAA mg/L	1/2MS	MS	1/2MS	MS	1/2MS	MS
1.0	0.5	91.7^b	93.3^b	95.0^ab	95.0^a	93.3^b	95.0^a
1.0	0.1	95.0^ab	96.7^ab	93.3^ab	96.7^a	95.0^ab	95.0^a
0.5	0.5	95.0^ab	98.3^ab	95.0^ab	96.7^a	93.3^b	96.7^a
0.5	0.1	96.7^ab	96.7^ab	100.0^a	100.0^a	100.0^a	100.0^a
0.1	0.1	100.0^a	100.0^a	100.0^a	100.0^a	100.0^a	100.0^a

BAP mg/L	NAA mg/L	single node cuttings	terminal buds	shoot cuttings
BAP mg/L		1/2MS	MS	1/2MS	MS	1/2MS	MS
No. of shoots per explant
1.0		1/2MS	MS	1/2MS	MS	1/2MS	MS	0.5	2.7^bc	6.5^a	5.3^ab	6.5^ab	7.1^abc	9.1^abc
1.0	0.1	2.5^bc	5.9^ab	4.8^bc	6.3^ab	6.7^bc	8.3^bc
0.5	0.5	3.0^bc	6.9^a	5.8^ab	6.5^ab	9.4^ab	12.1^ab
0.5	0.1	5.1^a	6.0^ab	5.9^ab	7.2^a	10.3^a	15.4^a
0.1	0.1	4.1^ab	4.6^ab	6.9^a	4.7^bc	6.0^bc	7.0^bc
No. of nodes per explant
1.0	0.1	4.1^ab	4.6^ab	6.9^a	4.7^bc	6.0^bc	7.0^bc	0.5	2.5^ab	7.1^a	3.2^a	6.8^ab	2.7^a	6.5^b
1.0	0.1	2.7^ab	3.4^c	1.5^b	5.2^bc	1.3^c	6.2^b
0.5	0.5	3.0^a	6.6^a	1.2^b	6.9^ab	1.5^c	9.5^a
0.5	0.1	1.0^c	6.2^ab	2.1^ab	8.5^a	1.3^c	9.7^a
0.1	0.1	1.4^c	6.0^ab	1.1^b	8.3^a	1.6^bc	8.2^ab

BAP mg/L	NAA mg/L	single node cuttings (mm)	terminal buds (mm)	shoot cuttings (mm)
BAP mg/L	NAA mg/L	1/2MS	MS	1/2MS	MS	1/2MS	MS
1.0	0.5	3.2^ab	3.0^ab	2.9^b	3.3^ab	3.1^b	3.0^b
1.0	0.1	2.3^b	2.2^b	2.3^bc	2.0^c	2.5^bc	2.2^bc
0.5	0.5	3.1^ab	3.1^ab	2.7^b	2.9^ab	2.7^bc	3.2^b
0.5	0.1	2.5^b	2.3^b	2.4^bc	2.6^b	2.4^c	2.4^bc
0.1	0.1	3.9^a	4.2^a	4.1^a	3.8^a	3.8^a	4.0^a

NAA (mg/L)	Medium	Rooting percentage (%)	No. of roots per explant	Mean length of the longest root (mm)
0.0	MS	88.3^ab	14.3^a	38.2^a
0.1	MS	86.7^ab	15.6^a	36.2^a
0.5	MS	95.0^a	15.1^a	31.5^a
1.0	MS	96.7^a	16.8^a	33.5^a
0.0	1/2MS	78.4^b	10.3^ab	22.1^b
0.1	1/2MS	78.4^b	9.8^ab	26.4^ab
0.5	1/2MS	83.4^b	11.8^ab	28.8^ab
1.0	1/2MS	85.0^ab	12.5^a	27.0^ab

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[TFN01] Note: The values followed by different letters are significantly different at the P < 0.05 level according to the LSD test.