Working Optimally with Serial Sections in Glycol Methacrylate Resin

The present work presents an optimized form for distributing serial sections in a glycol methacrylate slide set. This consists of filling the first row with sections from each slide, thus proceeding in the same way with the other rows. Therefore each slide will contain sections of three distinct sample segments.

Key words:
cytology; histology; historesin; microscopy; plant anatomy; technique

Authorship

Carlos André Espolador Leitão ^**Author for correspondence: candreel@yahoo.com.br

Universidade Estadual do Sudoeste da Bahia - Departamento de Ciências Naturais, Vitória da Conquista, Bahia, BrasilUniversidade Estadual do Sudoeste da BahiaBrazilVitória da Conquista, Bahia, BrazilUniversidade Estadual do Sudoeste da Bahia - Departamento de Ciências Naturais, Vitória da Conquista, Bahia, Brasil

^*Author for correspondence: candreel@yahoo.com.br

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Figure 1
Schematics of the serial sections disposal of a sample included in historesin. A- Slide illustrating the criteria for the sections arrangement. B- Slide containing 15 sections with their respective coordinates. C- Historesin block in side view, containing a fusiform sample positioned in order to obtain longitudinal sections. The sample is divided into segments (I to VI), each one possessing a different color. These colors correspond to those shown in the sections in the slide sets in D and G. D- A 6 slides set containing serial sections obtained from the sample in C, in the traditional arrangement, where each slide has the sections of a single segment. E- Histological slide containing five elliptical drops of water in row A. There will be placed a section on each drop. F- Same as in E, but with a rectilinear drop of water for the accommodation of the five sections. G- A 6 slides set containing the sections obtained from the sample shown in C, in an optimized arrangement. In this case, the rows A, B and C of the slides 1-3 contain the sections of the segments I, III and V, respectively; whereas on the slides 4-6 these same rows contain the sections of the segments II, IV and VI, respectively.

Figure 1 Schematics of the serial sections disposal of a sample included in historesin. A- Slide illustrating the criteria for the sections arrangement. B- Slide containing 15 sections with their respective coordinates. C- Historesin block in side view, containing a fusiform sample positioned in order to obtain longitudinal sections. The sample is divided into segments (I to VI), each one possessing a different color. These colors correspond to those shown in the sections in the slide sets in D and G. D- A 6 slides set containing serial sections obtained from the sample in C, in the traditional arrangement, where each slide has the sections of a single segment. E- Histological slide containing five elliptical drops of water in row A. There will be placed a section on each drop. F- Same as in E, but with a rectilinear drop of water for the accommodation of the five sections. G- A 6 slides set containing the sections obtained from the sample shown in C, in an optimized arrangement. In this case, the rows A, B and C of the slides 1-3 contain the sections of the segments I, III and V, respectively; whereas on the slides 4-6 these same rows contain the sections of the segments II, IV and VI, respectively.

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Working Optimally with Serial Sections in Glycol Methacrylate Resin

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[1] ^*Author for correspondence: candreel@yahoo.com.br