Insecticidal Activity of Lavandula dentata L. Essential Oil on Anticarsia gemmatalis (Hübner, 1818)

Vicenço, Camila Bonatto; Silvestre, Wendel Paulo; Menegol, Iriane Viganó; Carraro, Marina Cichin; Pauletti, Gabriel Fernandes

doi:10.1590/1678-4324-2021210327

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Article - Biological and Applied Sciences • Braz. arch. biol. technol. 64 • 2021 • https://doi.org/10.1590/1678-4324-2021210327 copy

Insecticidal Activity of Lavandula dentata L. Essential Oil on Anticarsia gemmatalis (Hübner, 1818)

Authorship SCIMAGO INSTITUTIONS RANKINGS

HIGHLIGHTS

The insecticidal activity of L. dentata EO was tested on A. gemmatalis.
The calculated LC₅₀ of L. dentata EO was 0.197% v/v for A. gemmatalis.
The EO was applied on soybean leaves to verify its deterrent effect on the insect.
EO at 0.4% v/v has already had a deterrent effect on the caterpillars.

Abstract

Anticarsia gemmatalis, commonly known as soybean caterpillar, causes important economic losses in soybean crops. Synthetic pesticides are the standard practice to control this insect. However, the indiscriminate use of these substances has increased the resistance of this pest. Thus, it is necessary to search for different control alternatives that are also more environmentally friendly. The objective of this work was to evaluate the chemical composition of Lavandula dentata L. essential oil (EO) and its activity on A. gemmatalis. The major compounds of essential oil were 1,8-cineole (31.5 wt.%), camphor (16.6 wt.%), and fenchone (15.9 wt.%). Bioassays were performed with third-instar caterpillars. EO concentrations of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1.0% v/v were diluted in Tween-80^® 0.5% v/v and incorporated into the artificial diet given to caterpillars. Water, Tween-80^® 0.5% v/v, and novaluron 0.075% w/v were added as negative and positive controls. For the aversion tests, soybean leaf discs were immersed in solutions with 0.4, 0.6, 0.8, and 1.0% v/v of EO, plus a negative control (water), and offered to caterpillars. In the bioassay, 100% mortality was observed from the concentration 0.6% v/v of the essential oil of L. dentata in 24 h; without statistical difference from 0.4 and 0.5% v/v. There was no important change in mortality between 24 and 72 h. In the aversion test, all EO treatments caused deterrence of caterpillars when compared to control, but without difference between EO concentrations. It was observed that L. dentata EO can be used as an alternative in the control of A. gemmatalis.

Keywords:
alternative control; botanical insecticides; terpenes; soybean caterpillar; Integrated Pest Management

Component	Calc. LRI¹	Lit. LRI²	Content (wt.%)
α-pinene	1018	1022	0.33±0.15
camphene	1062	1065	0.49±0.07
β-pinene	1106	1108	3.32±0.68
1,8-cineole	1213	1210	31.52±1.27
p-cymene	1275	1270	0.32±0.09
fenchone	1407	1410	15.93±0.75
cis-β-terpineol	1439	1437	0.24±0.03
α-camphonelal	1501	1504	0.29±0.12
camphor	1521	1515	16.63±2.11
linalool	1552	1551	1.54±0.35
pinocarvone	1581	1580	0.92±0.12
fenchol	1593	1597	6.46±0.77
terpinen-4-ol	1607	1600	0.36±0.06
myrtenal	1643	1639	0.97±0.15
trans-pinocarveol	1666	1664	2.59±0.66
α-terpineol	1712	1710	0.77±0.28
β-selinene	1732	1729	3.67±1.15
β-bisabolene	1728	1720	0.51±0.08
cuminal	1798	1794	0.49±0.11
myrtenol	1803	1807	0.58±0.25
cis-carveol	1844	1847	0.38±0.03
caryophyllene oxide	2004	2008	2.04±0.61
perillol	2018	2221	0.24±0.11
α-bisabolol	2222	2215	0.39±0.07
β-eudesmol	2247	2249	0.74±0.08
Monoterpenes			4.46
Oxygenated monoterpenes			79.91
Sesquiterpenes			4.18
Oxygenated sesquiterpenes			3.17
Not identified			8.28

Treatment	Mortality (%)
Treatment	24 h	48 h	72 h
Water	0.0±0.00^d	0.0±0.00^d	0.0±0.00^d
Tween-80^® 0.5% v/v	0.0±0.00^d	0.0±0.00^d	0.0±0.00^d
novaluron 0.075% w/v	0.0±0.00^d	93.2±0.82^a	100.0±0.00^a
0.1% v/v	23.2±0.52^c	26.6±0.52^c	26.6± 0.52^c
0.2% v/v	43.2±0.52^b	46.6±0.52^b	46.6±0.52^b
0.3% v/v	56.6±0.41^b	56.6±0.41^b	56.6±0.41^b
0.4% v/v	93.2±0.82^a	93.2±0.82^a	93.2±0.82^a
0.5% v/v	93.2±0.82^a	93.2±0.82^a	93.2±0.82^a
0.6% v/v	100.0±0.00^a	100.0±0.00^a	100.0±0.00^a
0.7% v/v	100.0±0.00^a	100.0±0.00^a	100.0±0.00^a
0.8% v/v	100.0±0.00^a	100.0±0.00^a	100.0±0.00^a
0.9% v/v	100.0±0.00^a	100.0±0.00^a	100.0±0.00^a
1.0% v/v	100.0±0.00^a	100.0±0.00^a	100.0±0.00^a

Treatment	RIR¹ (g∙g^-1∙day^-1)	Mortality² (%)
Water	6.7±3.87^b	20.0±0.05^d
0.4% v/v	-60.5±31.63^a	46.6±0.55^c
0.6% v/v	-22.3±14.87^a	53.2±0.57^b
0.8% v/v	-36.1±38.20^a	73.2±0.62^a
1.0% v/v	-186.8±33.23^a	73.2±0.48^a

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