Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension CulturesLentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
Key words:
Factor VIII; Hek293 cells; lentiviral vectors; polyethylenimine; serum-free suspension culture; transient transfection
