Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes

Heavy metal toxicity in plants lead to accumulation of reactive oxygen species (ROS). Antioxidant enzyme system is also not able to revert altered ROS homeostasis. The present study reports the heavy metal induced ROS toxicity by up-regulating the expression of key antioxidant enzyme genes through Brassinosteroids pre-soaking treatment in radish.

Keywords:
Brassinosteroids; oxidative stress; antioxidant enzymes; gene expression

Authorship

Indu Sharma ^**Author for correspondence: indu.gndu@yahoo.com

DAV University - Department of Agriculture, Jalandhar, Punjuab, India. DAV UniversityIndiaJalandhar, Punjuab, IndiaDAV University - Department of Agriculture, Jalandhar, Punjuab, India.

Ashutosh Sharma

DAV University - Department of Agriculture, Jalandhar, Punjuab, India. DAV UniversityIndiaJalandhar, Punjuab, IndiaDAV University - Department of Agriculture, Jalandhar, Punjuab, India.

Pratap Pati

Guru Nanak Dev University - Department of Biotechnology, Amritsar, Punjab, India. Guru Nanak Dev UniversityIndiaAmritsar, Punjab, IndiaGuru Nanak Dev University - Department of Biotechnology, Amritsar, Punjab, India.

Renu Bhardwaj

Guru Nanak Dev University - Botanical and Environmental Sciences, Amritsar, Punjab, IndiaGuru Nanak Dev UniversityIndiaAmritsar, Punjab, IndiaGuru Nanak Dev University - Botanical and Environmental Sciences, Amritsar, Punjab, India

*Author for correspondence: indu.gndu@yahoo.com

SCIMAGO INSTITUTIONS RANKINGS

DAV University - Department of Agriculture, Jalandhar, Punjuab, India. DAV UniversityIndiaJalandhar, Punjuab, IndiaDAV University - Department of Agriculture, Jalandhar, Punjuab, India.

Figures | Tables

Figures (2)
Tables (1)

Thumbnail

Figure 1
An ethidium bromide stained agarose gel electrophoresis products from reverse transcriptase PCR of 7 days old radish seedlings exposed to distilled water (Control, CN), 0.5 mM Cd, 10^-7 M EBL (EBL), 10^-7 HBL (HBL), both 0.5 mM Cd and 10^-7 M EBL (Cd+EBL), both 0.5 mM Cd and 10^-7 M HBL (Cd+HBL) for various key antioxidant genes: (a) Cat1, (b) Cat2, (c) Cat3, (d) Cu/Zn-Sod, (e) Fe-Sod and (f) Mn-Sod. Results were first normalized to the housekeeping gene Tub2, and then the relative expression of genes under various treatments was determined.

Thumbnail

Figure 2
An ethidium bromide stained agarose gel electrophoresis products from reverse transcriptase PCR of 7 days old radish seedlings exposed to distilled water (Control, CN), 1.0 mM Cr (Cd), 10^-7 M EBL (EBL), 10^-7 HBL (HBL), both 1.0 mM Cr and 10^-7 M EBL (Cr+EBL), both 1.0 mM Cr and 10^-7 M HBL (Cr+HBL) for various key antioxidant genes: (a) Cat1, (b) Cat2, (c) Cat3, (d) Cu/Zn-Sod, (e) Fe-Sod and (f) Mn-Sod. Results were first normalized to the housekeeping gene Tub2, and then the relative expression of genes under various treatments was determined.

Thumbnail

Table 1
List of Primers Used

Figure 1 An ethidium bromide stained agarose gel electrophoresis products from reverse transcriptase PCR of 7 days old radish seedlings exposed to distilled water (Control, CN), 0.5 mM Cd, 10^-7 M EBL (EBL), 10^-7 HBL (HBL), both 0.5 mM Cd and 10^-7 M EBL (Cd+EBL), both 0.5 mM Cd and 10^-7 M HBL (Cd+HBL) for various key antioxidant genes: (a) Cat1, (b) Cat2, (c) Cat3, (d) Cu/Zn-Sod, (e) Fe-Sod and (f) Mn-Sod. Results were first normalized to the housekeeping gene Tub2, and then the relative expression of genes under various treatments was determined.

Figure 2 An ethidium bromide stained agarose gel electrophoresis products from reverse transcriptase PCR of 7 days old radish seedlings exposed to distilled water (Control, CN), 1.0 mM Cr (Cd), 10^-7 M EBL (EBL), 10^-7 HBL (HBL), both 1.0 mM Cr and 10^-7 M EBL (Cr+EBL), both 1.0 mM Cr and 10^-7 M HBL (Cr+HBL) for various key antioxidant genes: (a) Cat1, (b) Cat2, (c) Cat3, (d) Cu/Zn-Sod, (e) Fe-Sod and (f) Mn-Sod. Results were first normalized to the housekeeping gene Tub2, and then the relative expression of genes under various treatments was determined.

Table 1 List of Primers Used

Primer

Nucleotide sequence of the Primers

1) CAT1
2) CAT1
3) CAT2
4) CAT2
5) CAT3
6) CAT3
7) Cu/Zn-SOD
8) Cu/Zn-SOD
9) Fe-SOD
10) Fe-SOD
11) Mn-SOD
12) Mn-SOD
13) TUB2
14) TUB2

F: CTGAACGTGAGACCAAGCATC
R: TAACAGCATGAGACAAAACCA
F: CGTCTCAATGTAAGGCCAAGCA
R: TCAAATAAAATAATAGTCGTCGAA
F: CTGAACGTGAGGCCAAGCATC
R: TAGTACTGCGTTTATTTTCATTGA
F: CCTGG(AC)CT(CT)CATGG(GC)TT(CT)CAT
R: CTGAG(ATG)TC(AG)TGTCC(AT)CCCTT
F: CT(CT)CC(AT)GC(AT)TTCAACAA(TC)GC
R: GTA(AT)GCATG(TC)TCCCA(AG)AC(AG)TC
F: GA(AG)GG(AT)GGTGG(GTC)GA(GA)CC(GAT)CC
R: GTA(AT)GCATG(TC)TCCCA(AG)AC(AG)TC
F: 5’-ATCCGTGAAGAGTACCCAGAT-3’
R: 5’-AAGAACCATGCACTCATCAGC-3’

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Brassinosteroids Reciprocates Heavy Metals Induced Oxidative Stress in Radish by Regulating the Expression of Key Antioxidant Enzyme Genes

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[1] *Author for correspondence: indu.gndu@yahoo.com