Raillietiella gigliolii ( PENTASTOMIDA ) INFECTING Amphisbaena alba ( SQUAMATA , AMPHISBAENIDAE ) : THE FIRST RECORD FOR NORTHEAST BRAZIL

Departamento de Sistematica e Ecologia, Universidade Federal da Paraiba, CCEN, Joao Pessoa, PB, BrazilCorrespondence to: Waltecio de Oliveira Almeida, Departamento de Ciencias Fisicas e Biologicas, Universidade Regional do Cariri – URCA/CE, Campus do Pimenta, Rua Cel. Antonio Luiz, 1161, CEP 63105-000, Crato, CE, Brazil, e-mail: walmeida@urca.brReceived March 30, 2005 – Accepted July 5, 2005 – Distributed November 1, 2006(With 1 figure)

The Raillietiellidae family is represented by the single pentastomid genus Raillietiella.There are records of parasitism for toads, lizards, serpents and amphisbaenids, showing evidence that there is quite a diversity of hosts (Almeida & Christoffersen, 1999, 2002).According to Almeida & Christoffersen (1999), Raillietiellidae is the sister group of the Cephalobaenidae and Reighardiidae families, representing the most basal clade of Pentastomida.
Circa 42 species of raillietiellids have been described.As general characters they present a cylindrical body, hooks forming a trapezoid design, parapodia present, rostrum reduced, cirri short, and fulcrum proportional to the size of the hooks (Riley, 1986;Almeida & Christoffersen, 1999, 2002).
Amphisbaenids were collected between January and August, 2004 in two sites at the Floresta Nacional do Araripe (FLONA) (07° 16' S and 39° 26' W), a protected environmental area of the 'IBAMA -Institute for the Environment and Natural Resources', municipality of Crato, State of Ceará, Northeast Brazil.The amphisbaenids were captured by using the classical herpetological methods of active patterned collecting (Vanzolini & Papavero, 1967;Auricchio & Salomão, 2002).After collection, the amphisbaenids were euthanised with ether, fixed and preserved in ethanol 70%.They were subsequently identified according to Barros-Filho & Valverde (1996) and Vanzolini (2002).The snout-vent length (SVL) was measured in each specimen using a caliper in centimeters.
The respiratory tracts of the amphisbaenids were removed and pentastomids were searched for under a stereomicroscope.The pentastomids that were found were cleared in the Hoyer's medium and slide-mounted.Identification followed on the basis of the dimensions of the hooks in males and copulatory spicule by using a microscope with micrometric ocular (see Ali et al. 1984Ali et al. , 1985)).A figure was made with a drawing tube connected to a brightfield microscope.The eco-parasitological terms used followed Bush et al. (1997).
A total of three female specimens of Amphisbaena alba (SVL range 29-82 cm) were collected and deposited in the collection of the Coleção do Laboratório de Zoologia da Universidade Regional do Cariri (LZ-URCA 0021, 0022, 0023).Among the three specimens of A. alba which were analysed, one presented three male specimens of Raillietiella gigliolii (LZ-URCA 0099, 0100, 0101) infecting the respiratory tract.This is the first record of R. gigliolii for the northeast region of Brazil.The infection patterns had a prevalence of 33.3% and mean intensity of 3.0 ± 0.0 (s = 3).
The raillietiellids encountered were characterized as adult specimens on the basis of their simple hooks.Nymphs are characterized by the presence of double hooks with chitinous accessory structures (see Ali et al. 1984;Riley 1986).The body length of the good preserved condition specimen of R. gigliolii is 9.5 mm.Other characters of the examined material are: abdomen without distinct annuli; cephalothorax trapezoidal, slightly wider than abdomen (Fig. 1a); hooks simple, sharp, anterior hook with AB 158 µm and BC 106 µm; posterior hook with AB 179 µm and BC of 129 µm (Fig. 1b); apical and dorsal papillae present and prominent; copulatory spicule is strongly curved and flares gradually into a trumpetshaped base; anus ventral; caudal papillae present.
Amphisbaenidae is well represented in Brazil, with four genera (Amphisbaena, Anops, Bronia, and Cercolophia) and approximately 44 described species (Barros-Filho & Valverde 1996).Parasitological surveys for identifying the most common endoparasites of these reptiles have not been conducted in detail.The basic food items of amphisbenids include insects such as termites and ants (personal observations).These arthropod food items should be the main transmission vehicles for pentastomid parasites (Ali et al., 1984(Ali et al., , 1985)).
Acknowledgments -Thank you to FUNCAP, Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico, for the research grant (Ref.943/03 -Edital 003/03) and scholarship of Felipe Silva Ferreira.Regards to CNPq, Brazilian National Research Council for a scholarship to Samuel Vieira Brito and a productivity fellowship to Martin Lindsey Christoffersen.Appreciation to IBAMA, Brazilian Institute for the Environment and Natural Resources, for the permission to collect samples from preserved areas (080/04 -RAN/ IBAMA Processo 02007.001009/04-73).We also thank one anonymous reviewer for valuable suggestions and criticisms on the manuscript, and to Prof. Dr. Jörn Seemann (URCA) for revising the English version of the text.