Figure 1
PCR amplification of patterns of rice breeding lines and parent genotypes using marker RM1377 closely linked with QTL qPSST-7. M-Marker 100 bp, 1-Avangard, 2-Liman, 3-Jinbabyeo, 4-Lazurnyi, 5-Mustakillik, 6-Avangard×KazNIIR-5, 7-Odaebyeo, 8-Opytnyi×KazNIIR-5, 9-Altynai×Opytnyi, 10-KazNIIR-5×Opytnyi, 11-KazNIIR-5×Liman, 12-KazNIIR-5×Altynai, 13-Liman×Jinbabyeo, 14-Lazurnyi×KazNIIR-5, 15-Jinbabyeo×Avangard, 16-Mustakillik×Avangard, 17-Kuban 3, 18-Marzhan, 19-Altynai, 20-Bakanasski, 21-UzROS 7-13, 22-Opytnyi×Kuban 3, 23-Opytnyi×Marzhan, 24-Kuban 3×Opytnyi, 25-Kuban 3×Liman, 26-Kuban 3×KazNIIR-5, 27-Kuban 3×Altynai, 28-Altynai×Kuban 3, 29-Marzhan×Liman, 30-Marzhan×Kuban 3, 31-KazNIIR-5×Kuban 3, 32-UzROS 7-13×Marzhan, 33-Avangard×Opytnyi, 34-Madina, 35-Ko 293 (IRRI mutant), 36-Opytnyi×Madina, 37-Opytnyi×Aru, 38-Odaebyeo×Madina, 39-Krepysh×Ko 293 (IRRI mutant), 40-FL478×Reizig, 41-FL 478×Todorokiwase.
Figure 2
Laboratory screening of rice breeding lines to cold resistance: planting seeds in vegetation vessels (A); seedlings at the V3-V4 stage of organogenesis in the greenhouse (B); cold treatment of genotypes in a climate chamber (t +10°C) (C); genotypes after cold treatment (43 days of vegetation) (D).
Figure 3
Assessment of cold resistance of rice breeding lines.
Figure 4
Screening of rice breeding lines to blast resistance by using molecular markers: (A) Pi-ta, Pita3 (corresponding to Pi-ta and Pita genes), (B) RM224 and RM1233 (Pi-1 gene), (C) MSM6 and 9871.T7E2b (Pi-40 gene), (D) 195R-1 and NMSMPi9-1 (Pi-9 gene) and (E) TRS26 and Pikh MAS (Pi-54 gene). M – 100 bp molecular marker, 1 - positive control, 2 – Jinbabyeo, 3 – Lazurnyi, 4 – Marzhan, 5 – Odaebyeo, 6 – Mustakillik, 7 – Kuban 3, 8 – UzROS 7-13, 9 - Avangard×KazNIIR-5, 10 - Opytnyi×KazNIIR-5, 11 - Altynai×Opytnyi, 12 - Kaz-NIIR-5×Opytnyi, 13 - KazNIIR-5×Liman, 14 - KazNIIR-5×Altynai, 15 - Lazurnyi×KazNIIR-5, 16 - Jinbabyeo×Avangard, 17 - Mustakillik×Avangard, 18 - Opytnyi×Kuban 3, 19 - Opytnyi×Marzhan, 20 - Kuban 3×Opytnyi, 21 - Kuban 3×Liman, 22 - Kuban 3×KazNIIR-5, 23 - Kuban 3×Altynai, 24 - Altynai×Kuban 3, 25 - Marzhan×Kuban 3, 26 - KazNIIR-5×Kuban 3, 27 - UzROS 7-13×Marzhan, 28- FL478×Reizig, 29 - FL478×Todorokiwase, 30 – Nippon bare (negative control).
Figure 5
Analysis of RBLs for the main yield trait “Weight of 1000 seeds”. 1 - Avangard×KazNIIR-5, 2 - Avangard Opytnyi, 3 – Opytnyi×Kuban 3, 4 – Opytnyi×Madina, 5 – Opytnyi×Aru, 6 – Opytnyi×Marzhan, 7 – Opytnyi×KazNIIR-5, 8 – Kuban 3×Opytnyi, 9 – Kuban 3×Liman, 10 – Kuban 3×KazNIIR-5, 11 – Kuban 3×Altynai, 12 – Altynai×Opytnyi, 13 – Altynai×Kuban 3, 14 – Marzhan×Liman, 15 – KazNIIR-5×Kuban 3, 16 – KazNIIR-5×Opytnyi, 17 – КазНИИР-5 /Лиман, 18 – KazNIIR-5×Altynai, 19 – Лиман/Jinbabyeo, 20 – Odaebyeo×Madina, 21 – Lazurnyi×KazNIIR-5, 22 – UzROS 7-13×Marzhan, 23 – Krepysh×Ко 293 (IRRI), 24 – Marzhan×Kuban 3, 25 – Jinbabyeo×Avangard, 26 – Mustakillik×Avangard, 27 – FL478×Reizig, 28 – FL478×Todorokiwase, 29 – Bakanasski.
Figure 2
Laboratory screening of rice breeding lines to cold resistance: planting seeds in vegetation vessels (A); seedlings at the V3-V4 stage of organogenesis in the greenhouse (B); cold treatment of genotypes in a climate chamber (t +10°C) (C); genotypes after cold treatment (43 days of vegetation) (D).
Figure 3
Assessment of cold resistance of rice breeding lines.
Figure 4
Screening of rice breeding lines to blast resistance by using molecular markers: (A) Pi-ta, Pita3 (corresponding to Pi-ta and Pita genes), (B) RM224 and RM1233 (Pi-1 gene), (C) MSM6 and 9871.T7E2b (Pi-40 gene), (D) 195R-1 and NMSMPi9-1 (Pi-9 gene) and (E) TRS26 and Pikh MAS (Pi-54 gene). M – 100 bp molecular marker, 1 - positive control, 2 – Jinbabyeo, 3 – Lazurnyi, 4 – Marzhan, 5 – Odaebyeo, 6 – Mustakillik, 7 – Kuban 3, 8 – UzROS 7-13, 9 - Avangard×KazNIIR-5, 10 - Opytnyi×KazNIIR-5, 11 - Altynai×Opytnyi, 12 - Kaz-NIIR-5×Opytnyi, 13 - KazNIIR-5×Liman, 14 - KazNIIR-5×Altynai, 15 - Lazurnyi×KazNIIR-5, 16 - Jinbabyeo×Avangard, 17 - Mustakillik×Avangard, 18 - Opytnyi×Kuban 3, 19 - Opytnyi×Marzhan, 20 - Kuban 3×Opytnyi, 21 - Kuban 3×Liman, 22 - Kuban 3×KazNIIR-5, 23 - Kuban 3×Altynai, 24 - Altynai×Kuban 3, 25 - Marzhan×Kuban 3, 26 - KazNIIR-5×Kuban 3, 27 - UzROS 7-13×Marzhan, 28- FL478×Reizig, 29 - FL478×Todorokiwase, 30 – Nippon bare (negative control).
Figure 5
Analysis of RBLs for the main yield trait “Weight of 1000 seeds”. 1 - Avangard×KazNIIR-5, 2 - Avangard Opytnyi, 3 – Opytnyi×Kuban 3, 4 – Opytnyi×Madina, 5 – Opytnyi×Aru, 6 – Opytnyi×Marzhan, 7 – Opytnyi×KazNIIR-5, 8 – Kuban 3×Opytnyi, 9 – Kuban 3×Liman, 10 – Kuban 3×KazNIIR-5, 11 – Kuban 3×Altynai, 12 – Altynai×Opytnyi, 13 – Altynai×Kuban 3, 14 – Marzhan×Liman, 15 – KazNIIR-5×Kuban 3, 16 – KazNIIR-5×Opytnyi, 17 – КазНИИР-5 /Лиман, 18 – KazNIIR-5×Altynai, 19 – Лиман/Jinbabyeo, 20 – Odaebyeo×Madina, 21 – Lazurnyi×KazNIIR-5, 22 – UzROS 7-13×Marzhan, 23 – Krepysh×Ко 293 (IRRI), 24 – Marzhan×Kuban 3, 25 – Jinbabyeo×Avangard, 26 – Mustakillik×Avangard, 27 – FL478×Reizig, 28 – FL478×Todorokiwase, 29 – Bakanasski.