New record of four sand fly species ( Diptera , Psychodidae ) in Rondônia State , Western Amazon , Brazil

aPrograma de Pós-graduação em Biologia Experimental, Fundação Universidade Federal de Rondônia – UNIR, Rod. BR 364, Km 9,5, CEP 76801-059, Porto Velho, RO, Brasil bLaboratório de Entomologia, Fundação Oswaldo Cruz – Fiocruz Rondônia, Rua da Beira, 7671, CEP 76812-245, Porto Velho, RO, Brasil cInstituto Nacional de Epidemiologia da Amazônia Ocidental – INCT-EpiAMO, Rua da Beira, 7671, CEP 76812-245, Porto Velho, RO, Brasil dLaboratório de Epidemiologia Genética, Fundação Oswaldo Cruz – Fiocruz Rondônia, Rua da Beira, 7671, CEP 76812-245, Porto Velho, RO, Brasil

(With 2 figures) Sand flies are insects of medical importance that are present in high diversity in the Neotropical region.In Brazil, approximately 280 species have been recorded (Galati, 2018) and 13 of these species are proven vectors of Leishmania spp.(Rangel et al., 2018).Prior to this study, 131 species had been recorded in Rondônia State (Galati, 2018).
This study presents new records of four sand fly species found in Rondônia State.These records are of male specimens collected between 2016 and 2017 using HP light traps.Collections were made in three protected areas that are characterized by dense ombrophylous forest: 1-Jamari National Forest, Itapuã do Oeste municipality, 2-Jaru Biological Reserve, Vale do Anari and Ji-Paraná municipalities, 3-Guajará-Mirim State Park, Nova Mamoré municipality.Collections were also made in forest fragments near dwellings in periurban areas located in the municipalities of Cacoal and Monte Negro (Figure 1).
Trichopygomyia wagleyi (Causey & Damasceno, 1945) (2 ♂, Figure 2F) has been recorded in Amazonas State, primarily in forest fragments (Pereira Júnior et al., 2015).In a recent survey of the road that connects Amazonas and Rondônia states, this species was found in a forest edge near peridomiciles (Julião et al., 2019) and in upland forest areas near small farms in a municipality along the middle Solimões River (Pereira Júnior et al., 2015).This species has also been recorded in tropical rain forests in Venezuela, Colombia and Bolivia (Galati, 2018).In our study, this species was found in the National Forest of Jamari (62°54'48.30"W,9°15'36.10"S), a conservation unit characterized by dense ombrophylous forest.
This study increases the number of species recorded in Rondônia to 135, an increase of nearly 4%, and it updates the number of species from genera found in Rondônia to: Brumptomyia (6), Nyssomyia (9), Psathyromyia (31) and Trichopygomyia (6).Psathyromyia elizabethdorvalae is characterized by epandrial lobes 1.2 times longer than the gonocoxite; parameres simple with dorsal margin presenting slight curvature preceding apex, edeagal ducts length 1.9 times longer than genital pump and gonostyle with four well developed spines (C).Nyssomyia delsionatali is differentiated by parameres with apex slightly curved (D) and gonocoxite bearing a group of less than 25 setae (E).Trichopygomyia wagleyi is identified by a paramere format in which the dorsal part is more dilated than the base (F).

Figure 1 .
Figure 1.Collection points of the new sand fly records to Rondônia State, Brazil.In the map (A) are indicated with the green color the municipalities and with yellow circles are highlighted the collection points of sand flies.In the map (B) is highlighted the Rondônia State territory in Brazil.

Figure 2 .
Figure 2. Male genitalia from the new sand fly records for Rondônia State.The identification of Brumptomyia mesai is based on its gonocoxite which bears a basal tuft of setae (A), and five to six setae in the apex region of this structure (B).Psathyromyia elizabethdorvalae is characterized by epandrial lobes 1.2 times longer than the gonocoxite; parameres simple with dorsal margin presenting slight curvature preceding apex, edeagal ducts length 1.9 times longer than genital pump and gonostyle with four well developed spines (C).Nyssomyia delsionatali is differentiated by parameres with apex slightly curved (D) and gonocoxite bearing a group of less than 25 setae (E).Trichopygomyia wagleyi is identified by a paramere format in which the dorsal part is more dilated than the base (F).