Synthesis, characterization and protection effect of black rice anthocyanins nano-composite against hepatotoxicity induced by methotrexate in rats

Síntese, caracterização e efeito protetor de nanocompósito de antocianinas de arroz preto contra hepatotoxicidade induzida por metotrexato em ratos

Abstract

The present study aimed to investigate the beneficial of prepared black rice anthocyanins nano-composite (An-AgNp_s) against hepatotoxicity induced by methotrexate (MTX) in rats. Anthocyanins nano-composite was prepared by silver as the metallic ion reduction and were characterized by IR and SEM. The rats in our experiment were divided into five groups. Serum lipid profile, serum transaminases (ALT and AST), ALP, LDH, TBA, GSH and SOD were examined. The results show that SEM of An-AgNp_s has average particle size from 70 to 130nm. In the group treated with MTX; TC, TG, LDL-c, ALT, AST, ALP, LDH and TBA levels were significantly (P≤0.05) increased than NC, while, HDL-c, SOD and GSH levels were significantly (P≤0.05) decreased. On the other hand, An-AgNp_s + MTX treated groups were reversed the levels of all biomarkers similar to NC. In conclusion, the results show that An-AgNp_s has a protective effect on MTX-induced hepatotoxicity and oxidative stress.

Keywords:
anthocyanins nano-composite; hepatotoxicity; methotrexate; black rice; infrared spectroscopy; scanning electron microscopy