Chemical defense in the red seaweed Plocamium brasiliense : spatial variability and differential action on herbivores

Species of Plocamium are known as prolific sources of halogenated secondary metabolites exhibiting few explored ecological roles. In this study the crude extracts from specimens of P. brasiliense collected in two distinct places, Enseada do Forno and Praia Rasa, Búzios, Estado do Rio de Janeiro, were evaluated as defense against the sea urchin Lytechinus variegatus and the crab Acanthonyx scutiformis. These specimens produce a similar amount of crude extract and also halogenated monoterpene compound-types, but individuals of P. brasiliense from Praia Rasa exhibit a major compound representing about 59% of the total chemicals. Natural concentrations of the crude extracts obtained from both specimens of P. brasiliense significantly inhibited the herbivory by the sea urchin L. variegatus, but had no significant effect on the feeding by A. scutiformis, a crab commonly associated to chemically defended host. Crude extract from P. brasiliense collected at Praia Rasa was more efficient as defense against L. variegatus than that crude extract from populations of this alga from Enseada do Forno, probably due to presence of a major secondary metabolite. These two studied population live under different environmental conditions, but they are only about 30 Km apart. However, it is impossible to affirm that environmental characteristics (abiotic or biotic) would be responsible for the difference of defensive potential found in the two populations of P. brasiliense studied here. Further genetic studies will be necessary to clarify this question and to explain why populations of a single species living in different but close locations can exhibit distinct chemicals.


Introduction
Chemical defenses of macroalgae have been extensively investigated during last 20 years, encompassing since species from Antarctic polar waters (Amsler et al., 2008) to those from the tropics (Pereira and Da Gama, 2008), and including several species of green, brown, and red ones.In fact, several species of macroalgae have the capacity to produce secondary metabolites, which exhibit important and vital ecological roles as defense and/or signal compounds (Amsler, 2008).
Green macroalgae, mainly species of Bryopsidales are abundant and widely distributed in tropical seas, and known to produce mainly sesquiterpenoid and diterpenoid compounds (Blunt et al., 2009, and previous reviews of this author).Some of these metabolites have been implicated in well documented studies that evidenced its property as a chemical defense against grazing by several species of fishes (Hay et al., 1990;Wylie and Paul, 1988), and invertebrates such as gastropods (Davis et al., 2005), mollusk (Pennings and Paul, 1992), and sea urchins (Hay et al., 1994;Lima et al., 2008).
Red species are the richest between other macroalgae in terms of secondary metabolite diversity and abundance (Blunt et al., 2009, and previous reviews of this author), encompassing more than 1500 different compounds belonging to all major classes of natural products (Maschek and Baker, 2008).Among these chemicals, isoprenoid and acetogenin derivatives are recognised as the primary class of defensive metabolites in these algae (Harper et al., 2001).In addition, red macroalgae are also characterised by the production of an impressive array of halogenated compounds exhibiting defensive properties against herbivory (Pereira and Da Gama, 2008).
Macroalgae secondary metabolites that deter herbivory can be qualitatively or quantitatively variable, but the intraspecific patterns of qualitative or quantitative variation in these chemicals are largely undocumented (but see Van Alstyne et al., 1989), and thus underappreciated in marine organisms.Due to the known broad ecological roles of macroalgae secondary metabolites (Hay and Fenical, 1988;Cronin, 2001;Paul et al., 2001), these variations may be of significant importance and/or have repercussions for population and community structure.
For example, terpenoid compounds in macroalgae occur in relatively low concentrations, ranging from 0.2% to 2.0% of algal dry mass (Paul andFenical, 1986, 1987).However, the variation in secondary metabolites content or compound-type may occur at a number of different levels: among individuals within a population (Sudatti et al., 2006) or among populations of the same species growing in different habitats (Pereira et al., 2000a(Pereira et al., , 2004)).
Species of Plocamium have been shown to be a rich source of terpenoids that vary for a given species depending on collection and season (Naylor et al., 1983).For example, different chemicals were verified in specimens of P. cartilagineum and P. violaceum, probably due to individual enzymatic processes (Mynderse and Faulkner, 1978).High chemical and diversity variability were also verified in major compounds of different populations of P. violaceum from California (Crews and Kho-Wiseman, 1977).Differences between specimens of different populations of P. violaceum (Faulkner, 1977) and P. cartilaginum (Higgs et al., 1977) were sufficient to propose the existence of chemotypes of these species along the littoral of California.Chemotypes of P. cartilagineum and P. hamatum were also verified along the littoral of Chile (San-Martin and Rovirosa, 1986;Rovirosa et al., 1988) and Australia (Coll et al., 1988;Konig et al., 1990), respectively.
In fact, a large variety of secondary metabolites has been isolated from Plocamium species from Antarctic (e.g.Rovirosa et al., 1990) to tropical regions (Capon et al., 1984), but very few studies have explored the potential ecological roles of these compounds and their contents.However, previous studies evidenced that chemicals from P. lepitophyllum and P. cartilagineum inhibit consumption by sea-urchin and gastropods (Sakata et al., 2000) and amphipod (Ankisetty et al., 2004).In addition, superficial chemicals from P. hamatum act as allelopathic in competitive interaction with coral (De Nys et al., 1991).
In order to ascertain the spatial variability in defensive chemicals among Brazilian populations of Plocamium brasiliense, the crude organic extracts and major secondary metabolites from two populations of this seaweed were investigated to specifically answer the following questions: 1) Is there quali-/quantitative variation in P. brasiliense secondary metabolites in two close but different places on the Brazilian littoral?2) Do these metabolites play a defensive role against consumers?3) Does metabolite variation impact herbivore defense of P. brasiliense in different places?

Organisms
Specimens of the red macroalga P. brasiliense were collected off the Brazilian coast -Enseada do Forno (22° 45' S, 41° 52' W) and Praia Rasa (22° 44' 10 S, 42° 57' 50 W), Búzios, Rio de Janeiro State, at depths ranging from 0.5 to 3.5 m in November, 2005.After being collected, these algal specimens were transported to the laboratory in ice cases, and then gently washed in seawater to eliminate macroscopic associated epibiota, as well as microorganisms loosely attached on its thalli.Voucher specimens were Chemical defense of Plocamium brasiliense deposited in the Herbarium of the Universidade do Estado do Rio de Janeiro (HRJ 10331-32).
Specimens of the sea urchin Lytechinus variegatus used in assays were collected in the sublittoral region at Itaipu Beach (Niterói, RJ -Brazil), while individuals of Acanthonyx scutiformis were collected at Praia Rasa (Búzios, RJ -Brazil).In the laboratory all these individuals were maintained in containers with seawater, where they were kept in a recirculating laboratory aquarium at constant temperature (20 °C), salinity (35) and aeration during the acclimation period, during which Ulva sp. was offered as food, before the assays.

Chemical procedures
The air-dried algae from Praia Rasa (423 g) and Enseada do Forno (240 g) were successively extracted with CH 2 Cl 2 at room temperature (25 °C), in an ultra-sound apparatus (3x1.0L for 3 min).The solvent was evaporated under reduced pressure yielding a yellowish residue (2.5 and 1.6 g, respectively), both used in feeding assays and also to analyse the chemical profiles.TLC of the extracts were performed on Merck Kieselgel GF 254 (0.2mm) precoated aluminum plates and spots were observed under UV light (254 nm) and visualised by spraying with a 2% ceric sulphate in sulfuric acid, followed by heating at 100 o C. Silica gel 60 (Merck, 70-230 and 230-400 mesh) were used for column chromatography.
The extracts were analysed by HRGC-MS (gas chromatographic analysis) on a HP 6890 series GC system, coupled to a HP 5973 mass selective detector in the electron impact mode (70 eV) equipped with an HP-1 MS capillary column (30 m x 0.25 mm; film thickness 0.25 μm).Injector and detector temperatures were set at 270 °C and 290 °C, respectively.The temperature program was kept at 60 °C, then programmed to 290 °C at a rate of 10 °C/min for 15 min.Hydrogen was the carrier gas at a flow rate of 1 mL/min.Diluted samples were injected manually in the split mode (1:10).Data were obtained from Frd area percent values.The chemical components were identified based on mass spectral comparison with those of standards and/or literature data, by co-injection in HRGC of these samples and from the Wiley 275 library data of the HRGC-MS system.

Feeding Experiments
The defensive property of the crude extracts and the major compound found in P. brasiliense, faced with the sea urchins Lytechinus variegatus and Acanthonyx scutiformis, were verified by including natural concentrations of each one of the components separately in an artificial food (Hay et al., 1994;Pereira et al., 2000b).The food (control) was prepared by adding 0.72 g of agar to 20.0 ml of distilled water and heating it in a microwave oven until boiling point.This mixture was added to 16.0 ml of cold water containing 2.0 g of the freeze-dried green seaweed Ulva spp.The experimental food (treatment) was similarly prepared, but the crude extract or pure compound of P. brasiliense was first dissolved in diethyl ether, added to 2.0 g of freeze-dried Ulva, and the solvent was then removed by rotary evaporation.This procedure is necessary to obtain a uniform coating of natural products on the algal particles prior to their addition to the agar (Hay et al., 1994).Treatments and controls were hardened onto a screen and cut into small pieces (7 x 10 squares about 1.2 x 1.4 mm each), which were then simultaneously offered to the sea urchin L. variegatus, in small plastic containers, each containing 250 ml of seawater (n = 18 to 22 replicates; see "Results").
Wilcoxon matched pairs test, a nonparametric equivalent to the t-test, was used to evaluate the statistical significance of the results obtained in the feeding assays.

Results and Discussion
Similar percentages of total crude extracts were obtained from specimens of P. brasiliense collected at Praia Rasa and Enseada do Forno (Table 1).In addition, the CCD, UV and HRGC-MS analysis confirmed that these specimens also produce similar compounds or exhibit the same or a very similar chemical profile (Figure 1).However, the extract of P. brasiliense from Praia Rasa showed a major compound representing about 59% of the total chemicals.In fact, a recent study revealed that both these populations of P. brasiliense produce halogenated monoterpenes, but while specimens from Praia Rasa showed only one halogenated monoterpene as major metabolite, individuals from Enseada do Forno exhibited several of these compound-types (Vasconcelos et al., 2010).These results confirm the ability of P. brasiliense to synthesise linear chlorinated and brominated monoterpenes, and also that Plocamium species produce variable contents of secondary metabolites depending on the collection location as previously observed worldwide (e.g.Crews et al., 1977;Mynderse and Faulkner, 1978;San-Martin and Rovirosa, 1986;Konig et al., 1990).
Natural concentrations of the crude extracts obtained from specimens of P. brasiliense from Enseada do Forno and Praia Rasa significantly inhibited herbivory by the sea urchin L. variegatus (p < 0.05, Wilcoxon paired-sample test, for both assays, Figure 2), confirming the predicted defensive property of the chemicals from this Brazilian red seaweed.These results also confirm previous evidence that Brazilian P. brasiliense was one of the last food items preferred by L. variegatus probably due to defensive chemicals produced by this macroalga (Souza et al., 2008).In general, chemicals from Plocamium species are very little explored in the context of its ecological roles, but it is known that crude extract of P. cartilagineum was significantly rejected by fish, sea star and amphipod
In contrast to the patterns verified for L. variegatus, crude extracts from specimens of P. brasiliense from Enseada do Forno and Praia Rasa had no significant effect on feeding by the crab A. scutiformis (Figure 3).In fact, this crab is a generalist feeder, but it exhibits some preference by the chemically-defended P. brasiliense and also uses this macroalga as decoration and probably protection against predation (Vasconcelos et al., 2009).In fact, this is a typical mechanism exhibited by small and more specialised marine organisms that live associated to and consume the chemically defended host to minimise predation (Hay, 1992).
When both extracts were simultaneously offered to this sea urchin, the crude extract from P. brasiliense collected at Praia Rasa was more efficient as a defense against L. variegatus than the crude extract from populations of this alga from Enseada do Forno (p < 0.05, Wilcoxon pairedsample test, Figure 2).Similarly, crude extract and major secondary metabolites from the brown alga Stypopodium zonale from two different places on the Brazilian littoral significantly deterred feeding by consumers, although with distinct effectiveness (Pereira et al., 2004).In fact, despite the small differences in the yields observed in our extracts, we can stress that defensive chemicals  Chemical defense of Plocamium brasiliense from macroalgae are not qualitatively or quantitatively absolute or invariant characteristics of the species, and may represent an ecological specialisation to successfully prevent herbivory (Pereira et al., 2004).
The major compound (substance 1), from specimens of P. brasiliense from Praia Rasa, exhibited a defensive property against L. variegatus (p < 0.05, Wilcoxon pairedsample test, Figure 4), and the action of the crude extract against this sea-urchin (Figure 2) is probable due to this major compound.By contrast, oregonene A also found in these specimens, but in a smaller amount, was ineffective against this sea urchin (p < 0.05, Wilcoxon paired-sample test, Figure 4).
In the marine environment, the knowledge of both the amount and variation in secondary metabolites is an essential element for assessing studies in chemical ecology and for placing them into an ecological and evolutionary context (de Nys et al., 1995(de Nys et al., , 1998)).Here, we have shown that close populations of P. brasiliense exhibit different chemicals and these components exhibit distinct action against herbivory.However, what determines this difference?
In general, it is unclear whether variations in concentration and types of secondary metabolites result from herbivore-induced chemical defenses, local selection, genetic differences, or other factors not related to herbivores (Paul et al., 2001).Many marine macroalgae show extensive phenotypic variation, including the production of defensive chemicals which can be generated by variation in a number of known biotic-herbivore pressure (e.g.Cronin, 2001 for review) and abiotic factors (e.g.Sudatti et al., 2011).
The two studied populations live under different environmental conditions, but they are only about 30 Km apart.Enseada do Forno is characterised by an environment under high wave energy, clear seawater, and subtidal dominance of the seaweeds Dictyota cervicornis Kuetzing, Sargassum furcatum Kuetzing, Stypopodium zonale (Phaeophyta) and Plocamium brasiliense (Greville) Howe et Taylor (Rhodophyta), and the sea urchins Echinometra  lucunter (Linnaeus) and Paracentrotus gaimardii (Blav.)Mortensen are the major invertebrate species (Sabino and Villaça, 1999).All these algal species are known to produce defensive chemicals against consumers (Pereira and Da Gama, 2008), and we could presuppose that they live under high herbivory pressure.On the other hand, Praia Rasa possesses turbid seawater and the most diverse algal flora of Rio de Janeiro state, and the sea-urchin Echinometra lucunter Linnaeus is usually found slightly above algal belts dominated by a dense cover of Sargassum spp.(Yoneshigue-Braga, 1985).However, Sargassum furcatum exhibits low levels of polyphenols, consistent with previous observations from other macroalgae from tropical regions (Targett and Arnold, 1998), and the natural levels of phenols found in this alga did not inhibited herbivory by Parhyale hawaiensis (Pereira and Yoneshigue-Valentin, 1999).According to these environmental characteristics we could presuppose that P. brasiliense from Enseada do Forno would be more defended than those specimens of this red alga from Praia Rasa.However, feeding experiments revealed just the opposite result.Thus, it is impossible to affirm that environmental characteristics (abiotic or biotic) would be responsible for the difference in defensive chemicals found in the two populations of P. brasiliense studied here.On the other hand, due to a distance of only about 30Km between Praia Rasa and Enseada do Forno, we could suppose high genetic similarity between specimens of P. brasiliense from these two places.However, further genetic studies will be necessary to clarify this question and to explain why populations of a single species living in different but closely located places can exhibit distinct chemicals.

Figure 1 .
Figure 1.Relative abundance of compounds found in crude extracts from specimens of P. brasiliense from Praia Rasa and Praia do Forno.

Figure 2 .
Figure 2. Effect of crude extracts from specimens of P. brasiliense collected at Praia Rasa and Forno on the consumption by the sea-urchin L. variegatus.

Figure 3 .
Figure 3.Effect of crude extracts from specimens of P. brasiliense collected at Praia Rasa and Forno on the consumption by the crab A. scutiformis.

Figure 4 .
Figure 4. Effect of pure compounds, oregonene and substance 1 from P. brasiliense on the consumption by the sea-urchin L. variegatus.

Table 1 .
Yield of crude extracts obtained from specimens of P. brasiliense collected in two distinct places, Praia Rasa and Enseada do Forno, Búzios, RJ, Brazil.