Genetic polymorphisms of LOC101800257 gene and its relationship with egg characteristics in Kerinci ducks (Anas Platyrhynchos)

Polimorfismos genéticos do gene LOC101800257 e sua relação com as características do ovo em patos Kerinci (Anas platyrhynchos)

Abstract

The Kerinci duck is a breed native of Jambi Province in Indonesia, is noteworthy for its various eggshell color. This study's primary objective was to explore the connection between genetic variations in the LOC101800257 gene and the eggshell color and egg weight of Kerinci ducks. For this investigation, a total of 46 Kerinci ducks were utilized for identification of the LOC101800257 gene polymorphisms using Sequencing analysis. The association between the LOC101800257 gene polymorphisms with the eggshell color and egg weight were analyzed using General Linear Model analysis. The research findings revealed the presence of two mutation sites, namely g.13993R (intron 11) and c.15504R / p.I435V (exon 9), within the LOC101800257 gene of Kerinci ducks. These mutations exhibited high genetic diversity, characterized by high values of polymorphic informative content (PIC) and nucleotide diversity (Nd), both exceeding 0.30 and 2.00, respectively. Furthermore, both mutation sites displayed a significant association (P<0.05) with the GG genotype which had a higher effect on egg weight. However, it is essential to note that these genetic variations in the LOC101800257 gene were not found to serve as genetic markers for eggshell color in Kerinci ducks. The LOC101800257 gene can be employed as a genetic marker in molecular selection processes to enhance the egg weight of Kerinci ducks.

Keywords:
eggshell color; haplotype; Kerinci ducks; LOC101800257 gene; sequencing