Aucula magnifica ( Schaus , 1904 ) ( Lepidoptera : Noctuidae : Agaristinae ) : morphology of egg and last instar larvae

Aiming to contribute to knowledge of the immature instars of Neotropical Lepidoptera, this study details the morphology of the egg and last instar larvae of Aucula magnifica (Schaus, 1904) (Lepidoptera: Noctuidae: Agaristinae), emphasising the structures of the corium and the chaetotaxy. There is also a report of the occurrence of entomopathogenic action of Nomuraea rileyi (Farlow) Samson fungi on the larva.

According to Poole (1989), Aucula magnifica was originally described by Schaus, in 1904, as Euthisanotia magnifica starting from specimens originating from Petrópolis, Rio de Janeiro, Brasil; Hampson (1910) complemented the original description of the adult starting from specimens from the British Museum.Silva et al. (1968) registered the occurrence of this species in Minas Gerais and Rio de Janeiro; recently, there were also made several registrations of the occurrence in Rio Grande do Sul (Specht and Corseuil, 1998;2002;Viana and Costa, 2001;Specht et al., 2005).
In spite of the frequent occurrence of A. magnifica in Rio Grande do Sul, little is known about the biology and morphology of the immature stages; this can also be said of the neotropical Agaristinae, standing out, only as references, publications on A. hilzingeri Berg, [1882] (Bourquin, 1958) and A. franclemonti Tood and Poole, [1981] (Rawlins, 1992).
Due to material readiness, in this study, the morphology of the egg and larva is detailed, also describing the occurrence of Nomuraea rileyi (Farlow) Samson fungi as a natural enemy of the larva.

Material and Methods
The immature stages of A. magnifica were obtained starting from a female collected on March 15 th , 2007, A. franclemonti (Rawlins, 1992).The sculptury (Figure 5) has a primary elevated rosette, formed by 16 sub triangular, elongated cells which have the minor angle directed to the mycropile, distal region related to the most elevated mycropile, which is highlighted as a crown.Secondary rosette with intermediate elevation between the primary one and the others; composed of 17 sub triangular cells, bigger and conspicuous, mainly at the distal region, related to the mycropilar area.Tertiary 15 rectangular-cell rosette, slightly narrower cells in the sense of mycropile; from which they become progressively more flat-in a luminous source near a vineyard in Faria Lemos, Bento Gonçalves, RS, by the second author.The insects were reared at the Laboratório de Biologia of the Campus Universitário da Região dos Vinhedos (CARVI), Universidade de Caxias do Sul (UCS), Bento Gonçalves, under 25 ± 1 °C, 70 ± 10% RU and 14 hours of photo phase; observations were carried out daily.
The eggs (Figure 2) were placed in Petri plates containing moistened filter paper.After the darkening, near eclosion, they were transferred to 200 mL glass flasks, containing Vitis labrusca Linn.-Vitaceae (grapevine branches) inserted in eppendorf tubes filled with water to maintain turgidity.The study of mycropile followed the method used by Angulo et al. (2006), which consisted of emptying the content of 10 eggs soon after the egg position, using an entomologic pin number 00.The corium were disposed in individual plates with a drop of glycerinated alcohol at 40%, covered with cover slips; they were photographed in a 400× optical microscope and from the picture, the drawing was elaborated (Figure 5).The morphology was compared to the description reported by A. franclemonti (Rawlins, 1992).
The description of the last larval instar (Figure 3) was made by auxilliary drawings, considering nomenclature and descriptions by Rawlins (1992), Merzheevskaya (1998) and Angulo et al. (2006), the setae maps of the thoracic and abdominal segments following the methodology described in Angulo et al. (2006), that consists of dissecting caterpillars of the last instar along the medium right dorsal line, submitting them to a clarification treatment through the action of a solution of KOH at 10%.The 10 caterpillars used in the elaboration of the drawings were submitted to this procedure, and the teguments were disposed among sheets, for observation under stereo microscope.
All the material used in this study is conserved and kept in the collection of the Laboratory.
Contributions for description of the larval morphology were obtained in works of general knowledge such as Crumb (1956), Stehr (1987), Kitching and Rawlins (1998); however, because of the proximity of taxonomy, most of the aspects of the chaetotaxy were compared with the aspects illustrated and described by A. hilzingeri (Bourquin, 1958), for A. franclemonti (Rawlins, 1992) and Spodoptera cosmioides (Walker, 1858;Zenker et al., 2007).

Results and Discussion
The eggs (Figure 2) are sub spherical, slightly flattened, and plane based; their diameter is 1.1 mm; translucent, sculptured corium.The shape is similar to that described by Rawlins (1992) for A. franclemonti eggs; however, soon after oviposition, they are whitish light green and at the end of incubation they become dark brown, as described for A. hilzigeri (Bourquin, 1958).They are individually deposited, in groups from three to ten, but never covered by pubescence, as observed for  tened and, from the sixth to the tenth rosette, they can divide in two (the main part of them) or in three radial crystals, very well defined, formed by elongated cells of variable shapes, having more elevated borders, together with which aeropyles are observed, generally alternated among cells on one or the other side.
In the ten eggs studied, 27 crests were observed, which is a higher number than the 22-25 found by Rawlins (1992) for A. franclemonti.In the same way, it was observed that in A. magnifica only half of the crests reach the mycropilar rosette too.
From the first instar, the larvae have a reddish-brown head and body tegument, with very big black pinaculae, with long and transparent bristles.Both thoracic legs and larvopodes are blackish.Mid-dorsal and sub-dorsal lines are whitish and interrupted in some segments.These characteristics are shared by A. hilzigeri (Bourquin, 1958) and A. franclemonti (Rawlins, 1992).
Head (Figure 3) hypognate, reddish-dark brown, often blacker at the extremity; brown clypeus, with dark brown ventral margin; light brown adfrontal area; light and well-defined ecdysal area; orange or yellowish vertex (epicranial notch) and gena; same colour for fore clypeous and antenna base; brown labrum, with darker superior portion.Adfrontal sutures along fore clypeous margin and epicranial suture towards the tail to the epicranial notch.
Regarding chaetotaxy, the head (Figures 6-8) has bristle A1 located between the adfrontal area and the third stem; bristle A2 located just above bristle A1 (right), between adfrontal area and the first stem; bristle A3 located just above the first stem, forming a triangle together with the other bristles of the former group; fore pore Aa above and between bristles A2 and A3. 3 stem or ocellar bristles: S1 above and between fourth and sixth stem; S2 lateral to first stem; S3 post-dorsal to sixth stem, between this and bristle S3, the pore is located Sa.Three sub stem bristles SS: SS1 more ventral and close to the jaw, SS2 between SS1 and SS3; SS3 more distal to antenna; sub stem pore Ssa between SS2 and SS3.Fore bristles (F) represented only by F1, in the front midinferior region; pore Fa more inferior and more central (medium) than F1, in the front.Two adfrontal bristles AF1 and AF2, being AF1 more medium and AF2 more superior; pore AFa between bristles AF1 and AF2.Two clypeus bristles (C): C1 located in the inferior vertex off clypeus and ventral to C2. Bristle L1 (lateral) at the genal portion of cephalic capsule; pore La on the right of L1.Hind bristles (P) at the superior face of the head, between the lateral and superior adfrontal region: P1 close to ecdysal line; P2 superior to P1; pore Pa inferior and ventral to P1 and L1; pore Pb superior to P1 and inferior on the left of P2.CD (cephalus-dorsal) or MD (microdorsal) according to Stehr (1987) are fairly short, located  Maxilar (Figure 9) similar to the one described for A. franclemonti (Rawlins, 1992) highlighting as main differences, the internal tooth which presents two tops and less developed hind condylus mandibles.
Similar to A. franclemonti (Rawlins, 1992), A. magnifica has a complex hypopharingeal (Figures 10-12) tubular with two projections between a longitudinal furrow in the dorsal area delimiting the spinner, with plain apix, differently from the wavy one in A. franclemonti (Rawlins, 1992).The structure of the antenna (Figure 13) match those of A. franclemonti (Rawlins, 1992).
A. magnifica (Figure 3) presents body epidermis ornamentation varying from light brown to black; mid-dorsal and lateral-dorsal lighter lines than body and spots, which is because they are little evident; brown prothoracic and anal plates, lighter than body; more conspicuous spiracular and sub-spiracular areas at first instars and less evident in the latter because they are darker; brown larvopods and dark brown thoracic legs; light and translucent bristles implanted in dark and black chalazas (Figure 14).
region, being the prothoracic plate immediately after the head.Very clear and conspicuous region between metathorax and the first abdominal segment in caterpillars at the first instars.At the last instars, colour becomes darker and plates more conspicuous.Light-coloured mid-dorsal and sub-spiracular stripe.Lighter than body belly.Prothorax spiracles and eighth uromere approximately twice as big and more conspicuous than the others.rated at mid and meta thorax.In T1 the thoracic plate has besides D1 and D2, fore dorsal vertical bristles (XD2 e XD1), respectively.Between XD2 and XD1 there are XDa and XDc pores, bigger ones near XD1, XDa further between D1 and XD2, there is XDb.At the margin of the plate, distal to the head, there is MXD1.Absent spiracles in T2-T3 and relatively conspicuous in T1, but smaller than in the eighth uromere.MD1 between D1 and D2 in T2 and D1 in T3, in an upper position, next to D1. Subdorsally, between SD2 from the segments T1-T2, there are inconspicuous MSD1 and MSD2, bent to T1. Between T2-T3, below MD1, MD2 highlights; with vertical bending related to mid thorax.The main part of the bristles is in the chalazas, significantly conspicuous or either inconspicuous.Rawlins (1992) attributed, for A. franclemonti, more emphasis to the presence of two SV bristles in the A1, two SV bristles in the A7 and a SD1 normal, less conspicuous bristle, in the A9.
Thoracic legs (Figures 16-17) similarly to A. franclemonti (Rawlins, 1992) have divided thighs in fore and hind sclerite in its anteroventral extended suture with coccyx condyle articulated with the trochanter.This also presents five primary coccyx bristles: one of them (C1) in the anterior coccyx sclerite and four (C2-Thorax chaetotaxy (Figure 15) is characterised by dorsal bristles (D) of similar length in all segments, corresponding to that represented for A. franclemonti (Rawlins, 1992); sub-dorsal bristles (SD1-SD2) in the prothorax (T1) close to thoracic plate directed horizontally with a light bending, of less than 45° directed to mesothorax (T2), being SD2 more conspicuous than SD1 in all segments; SD1-SD2 vertically lined in mid and metathorax (T2-T3); D1 and D2 vertically lined in T1-T3; two sub-belly bristles (SV) appeared in T1 and only one SV in the segments T2-T3; lateral bristles (L2) present at T1-T2 and L3 in the segments T2-T3; A. franclemonti has L2 only in T1 segment (Rawlins, 1992).SD1 and SD2 bristles in the same chalazae in the pro thorax sepa-    of detailed description of structures as egg and larvopods by Rawlins (1992).
The morphologic characterisation of the pupa phase was not done because all the specimens died before metamorphose, as they were attacked by an entomopathogenic fungus isolated and identified as Nomuraea rileyi (Farlow) Samson (Figure 4), kept in the Laboratório de Controle de Pragas of Instituto de Biotecnologia of Universidade de Caxias do Sul, under register number UCS 03.
Abdomen with dark conspicuous chalazes with big, translucent bristles.The mid-dorsal stripe is narrower and less evident than the latero-dorsal; the sub-spiracle one is clear and conspicuous, with some irregular points.It has larvopods from A3 to A6 and A10.According to Merzheevskaya (1998), the hooks are uniordinals, and are a total of 27 in each larvopod (Figure 18).
Regarding chaetotaxy, the abdome (Figure15) presents four bristles SV in the A10, three from the A2 to A6, two in the A1, seventh and A8 and one in the A9.Bristle L1 posterior to spiracle from A1 to A6 and A8.
This study showed a big similarity between last instar caterpillars from A. magnifica and A. franclemonti, but more comparisons were not done due to the absence