AmpC β-lactamase (Class C) |
MOX-1, MOX-2, CMY-1, CMY-8 to CMY-11
|
MOXM F GCT GCT CAA GGA GCA CAG GAT |
520 |
64 ºC (1) |
Pérez-Pérez and Hanson (2002)PEREZ-PEREZ, F.J. and HANSON, N.D., 2002. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology, vol. 40, no. 6, pp. 2153-2162. http://dx.doi.org/10.1128/JCM.40.6.2153-2162.2002. PMid:12037080. http://dx.doi.org/10.1128/JCM.40.6.2153-...
|
MOXM R CAC ATT GAC ATA GGT GTG GTG C |
LAT-1 to LAT-4, CMY-2 to CMY-7, BIL-1
|
CITM F TGG CCA GAA CTG ACA GGC AAA |
462 |
64 ºC (1) |
Pérez-Pérez and Hanson (2002)PEREZ-PEREZ, F.J. and HANSON, N.D., 2002. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology, vol. 40, no. 6, pp. 2153-2162. http://dx.doi.org/10.1128/JCM.40.6.2153-2162.2002. PMid:12037080. http://dx.doi.org/10.1128/JCM.40.6.2153-...
|
CITM R TTT CTC CTG AAC GTG GCT GGC |
DHA-1, DHA-2
|
DHAM F AAC TTT CAC AGG TGT GCT GGG T |
405 |
64 ºC (1) |
Pérez-Pérez and Hanson (2002)PEREZ-PEREZ, F.J. and HANSON, N.D., 2002. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology, vol. 40, no. 6, pp. 2153-2162. http://dx.doi.org/10.1128/JCM.40.6.2153-2162.2002. PMid:12037080. http://dx.doi.org/10.1128/JCM.40.6.2153-...
|
DHAM R CCG TAC GCA TAC TGG CTT TGC |
ACC
|
ACCM F AAC AGC CTC AGC AGC CGG TTA |
346 |
64 ºC (1) |
Pérez-Pérez and Hanson (2002)PEREZ-PEREZ, F.J. and HANSON, N.D., 2002. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology, vol. 40, no. 6, pp. 2153-2162. http://dx.doi.org/10.1128/JCM.40.6.2153-2162.2002. PMid:12037080. http://dx.doi.org/10.1128/JCM.40.6.2153-...
|
ACCM R TTC GCC GCA ATC ATC CCT AGC |
MIR-1T, ACT-1
|
EBCM F TCG GTA AAG CCG ATG TTG CGG |
302 |
64 ºC (1) |
Pérez-Pérez and Hanson (2002)PEREZ-PEREZ, F.J. and HANSON, N.D., 2002. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology, vol. 40, no. 6, pp. 2153-2162. http://dx.doi.org/10.1128/JCM.40.6.2153-2162.2002. PMid:12037080. http://dx.doi.org/10.1128/JCM.40.6.2153-...
|
EBCM R CTT CCA CTG CGG CTG CCA GTT |
FOX-1 to FOX-5b
|
FOXM F AAC ATG GGG TAT CAG GGA GAT G |
190 |
64 ºC (1) |
Pérez-Pérez and Hanson (2002)PEREZ-PEREZ, F.J. and HANSON, N.D., 2002. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. Journal of Clinical Microbiology, vol. 40, no. 6, pp. 2153-2162. http://dx.doi.org/10.1128/JCM.40.6.2153-2162.2002. PMid:12037080. http://dx.doi.org/10.1128/JCM.40.6.2153-...
|
FOXM R CAA AGC GCG TAA CCG GAT TGG |
extended spectrum β-lactamases (Class A) |
SHV
|
SHV F ATG CGT TATATT CGC CTG TG |
747 |
60 ºC (2) |
Paterson et al. (2003)PATERSON, D.L., HUJER, K.M., HUJER, A.M., YEISER, B., BONOMO, M.D., RICE, L.B., BONOMO, R.A., and INTERNATIONAL KLEBSIELLA STUDY GROUP, 2003. Extended spectrum betalactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV-and CTX-M type beta-lactamases. Antimicrobial Agents and Chemotherapy, vol. 47, no. 11, pp. 3554-3560. http://dx.doi.org/10.1128/AAC.47.11.3554-3560.2003. PMid:14576117. http://dx.doi.org/10.1128/AAC.47.11.3554...
|
SHV R TGC TTT GTT ATT CGG GCC AA |
TEM
|
TEM F TCG CCG CAT ACA CTA TTC TCA GAA TGA |
445 |
60 ºC (2) |
Monstein et al. (2007)MONSTEIN, H.J., OSTHOLM-BALKHED, A., NILSSON, M.V., NILSSON, M., DORNBUSCH, K. and NILSSON, L., 2007. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTXM genes in Enterobacteriaceae. APMIS. Acta Pathologica, Microbiologica, et Immunologica Scandinavica, vol. 115, no. 12, pp. 1400-1408. http://dx.doi.org/10.1111/j.1600-0463.2007.00722.x. PMid:18184411. http://dx.doi.org/10.1111/j.1600-0463.20...
|
TEM R ACG CTC ACC GGC TCC AGA TTT AT |
CTX
|
CTX-M F ATG TGC AGY ACC AGT AAR GTK ATG GC |
593 |
60 ºC (2) |
Boyd et al. (2004)BOYD, D.A., TYLER, S., CHRISTIANSON, S., MCGEER, A., MULLER, M.P., WILLEY, B.M., BRYCE, E., GARDAM, M., NORDMANN, P. and MULVEY, M.R., 2004. Complete nucleotide sequence of a 92-kilobase plasmid harbouring the CTX-M-15 extended spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Antimicrobial Agents and Chemotherapy, vol. 48, no. 10, pp. 3758-3764. http://dx.doi.org/10.1128/AAC.48.10.3758-3764.2004. PMid:15388431. http://dx.doi.org/10.1128/AAC.48.10.3758...
|
CTX-M R TGG GTR AAR TAR GTS ACC AGA AYC AGC GG |
KPC |
KPCM F CGTCTAGTTCTGCTGTCTTG |
789 |
60 ºC (2) |
Poirel et al. (2011)POIREL, L., WALSH, T.R., CUVILLIER, V. and NORDMANN, P., 2011. Multiplex PCR for detection of acquired carbapenemase genes. Diagnostic Microbiology and Infectious Disease, vol. 70, no. 1, pp. 119-123. http://dx.doi.org/10.1016/j.diagmicrobio.2010.12.002. PMid:21398074. http://dx.doi.org/10.1016/j.diagmicrobio...
|
KPCM R CTTGTCATCCTTGTTAGGCG |
extended spectrum β-lactamases (Class D) |
OXA
|
OXA F 5-ATT ATC TAC AGC AGC GCC AGT G-3 |
296 |
60 ºC (2) |
Kim et al. (2009)KIM, J., JEON, S., RHIE, H., LEE, B., PARK, M., LEE, H., LEE, J. and KIM, S., 2009. Rapid detection of extended spectrum β-lactamase (ESBL) for Enterobacteriaceae by use of a multiplex PCR-based method. Infection & Chemotherapy, vol. 41, no. 3, pp. 181-184. http://dx.doi.org/10.3947/ic.2009.41.3.181. http://dx.doi.org/10.3947/ic.2009.41.3.1...
|
OXA R 5-TGC ATC CAC GTC TTT GGT G-3 |
Metallo-β-lactamases (Class B) |
Imp
|
Imp-F 5-GGAATAGAGTGGCTTAAYTCTC-3 |
188bp |
52 ºC (3) |
Ellington et al. (2007)ELLINGTON, M.J., KISTLER, J., LIVERMORE, D.M. and WOODFORD, N., 2007. Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. The Journal of Antimicrobial Chemotherapy, vol. 59, no. 2, pp. 321-322. http://dx.doi.org/10.1093/jac/dkl481. PMid:17185300. http://dx.doi.org/10.1093/jac/dkl481...
|
Imp-R 5-CCAAACYACTASGTTATCT-3 |
Vim
|
Vim-F 5-GATGGTGTTTGGTCGCATA-3 |
390bp |
52 ºC (3) |
Ellington et al. (2007)ELLINGTON, M.J., KISTLER, J., LIVERMORE, D.M. and WOODFORD, N., 2007. Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. The Journal of Antimicrobial Chemotherapy, vol. 59, no. 2, pp. 321-322. http://dx.doi.org/10.1093/jac/dkl481. PMid:17185300. http://dx.doi.org/10.1093/jac/dkl481...
|
Vim-R 5-CGAATGCGCAGCACCAG-3 |
Gim
|
Gim-F 5-TCGACACACCTTGGTCTGAA-3 |
477bp |
52 ºC (3) |
Ellington et al. (2007)ELLINGTON, M.J., KISTLER, J., LIVERMORE, D.M. and WOODFORD, N., 2007. Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. The Journal of Antimicrobial Chemotherapy, vol. 59, no. 2, pp. 321-322. http://dx.doi.org/10.1093/jac/dkl481. PMid:17185300. http://dx.doi.org/10.1093/jac/dkl481...
|
Gim-R 5-AACTTCCAACTTTGCCATGC-3 |
Spm
|
Spm-F 5-AAAATCTGGGTACGCAAACG-3 |
271bp |
52 ºC (3) |
Ellington et al. (2007)ELLINGTON, M.J., KISTLER, J., LIVERMORE, D.M. and WOODFORD, N., 2007. Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. The Journal of Antimicrobial Chemotherapy, vol. 59, no. 2, pp. 321-322. http://dx.doi.org/10.1093/jac/dkl481. PMid:17185300. http://dx.doi.org/10.1093/jac/dkl481...
|
Spm-R 5-ACATTATCCGCTGGAACAGG-3 |
Sim
|
Sim-F 5-TACAAGGGATTCGGCATCG-3 |
570bp |
52 ºC (3) |
Ellington et al. (2007)ELLINGTON, M.J., KISTLER, J., LIVERMORE, D.M. and WOODFORD, N., 2007. Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases. The Journal of Antimicrobial Chemotherapy, vol. 59, no. 2, pp. 321-322. http://dx.doi.org/10.1093/jac/dkl481. PMid:17185300. http://dx.doi.org/10.1093/jac/dkl481...
|
Sim-R 5-TAATGGCCTGTTCCCATGTG-3 |
Integrases |
intI1
|
intI1 F 5-GCATCCTCGGTTTTCTGG-3 |
457bp |
58 ºC (4) |
Shibata et al. (2003)SHIBATA, N., DOI, Y., YAMANE, K., YAGI, T., KUROKAWA, H., SHIBAYAMA, K., KATO, H., KAI, K. and ARAKAWA, Y., 2003. PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by Gram-negative bacteria isolated in Japan, with focus on the class 3 integron. Journal of Clinical Microbiology, vol. 41, no. 12, pp. 5407-5413. http://dx.doi.org/10.1128/JCM.41.12.5407-5413.2003. PMid:14662918. http://dx.doi.org/10.1128/JCM.41.12.5407...
|
intI1 R 5-GGTGTGGCGGGCTTCGTG-3 |
intI2
|
intI2 F 5-CACGGATATGCGACAAAAAGG T-3 |
789bp |
58 ºC (4) |
Shibata et al. (2003)SHIBATA, N., DOI, Y., YAMANE, K., YAGI, T., KUROKAWA, H., SHIBAYAMA, K., KATO, H., KAI, K. and ARAKAWA, Y., 2003. PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by Gram-negative bacteria isolated in Japan, with focus on the class 3 integron. Journal of Clinical Microbiology, vol. 41, no. 12, pp. 5407-5413. http://dx.doi.org/10.1128/JCM.41.12.5407-5413.2003. PMid:14662918. http://dx.doi.org/10.1128/JCM.41.12.5407...
|
intI2 R 5-GTAGCAAACGAGTGACGAAATG-3 |
intI3
|
intI3 F 5-AT TGCCAAACCTGACTG-3 |
922bp |
58 ºC (4) |
Shibata et al. (2003)SHIBATA, N., DOI, Y., YAMANE, K., YAGI, T., KUROKAWA, H., SHIBAYAMA, K., KATO, H., KAI, K. and ARAKAWA, Y., 2003. PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by Gram-negative bacteria isolated in Japan, with focus on the class 3 integron. Journal of Clinical Microbiology, vol. 41, no. 12, pp. 5407-5413. http://dx.doi.org/10.1128/JCM.41.12.5407-5413.2003. PMid:14662918. http://dx.doi.org/10.1128/JCM.41.12.5407...
|
intI3 R 5-CGAATGCCCCAACAACTC-3 |
type III secretion system (T3SS) |
exoS
|
Exo S F: GCG AGG TCA GCA GAG TAT CG |
118 |
56ºC (5) |
Ajayi et al. (2003)AJAYI, T., ALLMOND, L.R., SAWA, T. and WIENER-KRONISH, J.P., 2003. Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system. Journal of Clinical Microbiology, vol. 41, no. 8, pp. 3526-3531. http://dx.doi.org/10.1128/JCM.41.8.3526-3531.2003. PMid:12904350. http://dx.doi.org/10.1128/JCM.41.8.3526-...
|
Exo S R: TTC GGC GTC ACT GTG GAT GC |
exoT
|
Exo T F: AAT CGC CGT CCA ACT GCA TGC G |
152 |
56ºC (5) |
Ajayi et al. (2003)AJAYI, T., ALLMOND, L.R., SAWA, T. and WIENER-KRONISH, J.P., 2003. Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system. Journal of Clinical Microbiology, vol. 41, no. 8, pp. 3526-3531. http://dx.doi.org/10.1128/JCM.41.8.3526-3531.2003. PMid:12904350. http://dx.doi.org/10.1128/JCM.41.8.3526-...
|
Exo T R: TGT TCG CCG AGG TAC TGC TC |
exoU
|
Exo U F: CCG TTG TGG TGC CGT TGA AG |
134 |
56ºC (5) |
Ajayi et al. (2003)AJAYI, T., ALLMOND, L.R., SAWA, T. and WIENER-KRONISH, J.P., 2003. Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system. Journal of Clinical Microbiology, vol. 41, no. 8, pp. 3526-3531. http://dx.doi.org/10.1128/JCM.41.8.3526-3531.2003. PMid:12904350. http://dx.doi.org/10.1128/JCM.41.8.3526-...
|
Exo U R: CCA GAT GTT CAC CGA CTC GC |
exoY
|
Exo Y F: CGG ATT CTA TGG CAG GGA GG |
289 |
56ºC (5) |
Ajayi et al. (2003)AJAYI, T., ALLMOND, L.R., SAWA, T. and WIENER-KRONISH, J.P., 2003. Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system. Journal of Clinical Microbiology, vol. 41, no. 8, pp. 3526-3531. http://dx.doi.org/10.1128/JCM.41.8.3526-3531.2003. PMid:12904350. http://dx.doi.org/10.1128/JCM.41.8.3526-...
|
Exo Y R: GCC CTT GAT GCA CTC GAC CA |
Fluoroquinolone |
GyrA
|
GyrA F: TTA TGC CAT GAG CGA GCT GGG CAA CGA CT |
366 |
65ºC |
Sekiguchi et al. (2005)SEKIGUCHI, J., ASAGI, T., MIYOSHI-AKIYAMA, T., FUJINO, T., KOBAYASHI, I., MORITA, K., KIKUCHI, T., KURATSUJI, T. and KIRIKAE, T., 2005. Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6′)-Iae gene cassette encoding a novel aminoglycoside acetyltransferase. Antimicrobial Agents and Chemotherapy, vol. 49, no. 9, pp. 3734-3742. http://dx.doi.org/10.1128/AAC.49.9.3734-3742.2005. PMid:16127047. http://dx.doi.org/10.1128/AAC.49.9.3734-...
|
GyrA R: AAC CGT TGA CCA GCA GGT TGG GAA TCT T |
ParC
|
ParC F: ATG AGC GAA CTG GGG CTG GAT |
210 |
58ºC |
Sekiguchi et al. (2005)SEKIGUCHI, J., ASAGI, T., MIYOSHI-AKIYAMA, T., FUJINO, T., KOBAYASHI, I., MORITA, K., KIKUCHI, T., KURATSUJI, T. and KIRIKAE, T., 2005. Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6′)-Iae gene cassette encoding a novel aminoglycoside acetyltransferase. Antimicrobial Agents and Chemotherapy, vol. 49, no. 9, pp. 3734-3742. http://dx.doi.org/10.1128/AAC.49.9.3734-3742.2005. PMid:16127047. http://dx.doi.org/10.1128/AAC.49.9.3734-...
|
ParC R: ATG GCG GCG AAG GAC TTG GGA |
ParE
|
ParE F: CGG CGT TCG TCT CGG GCG TGG TGA AGG A |
592 |
68ºC |
Sekiguchi et al. (2005)SEKIGUCHI, J., ASAGI, T., MIYOSHI-AKIYAMA, T., FUJINO, T., KOBAYASHI, I., MORITA, K., KIKUCHI, T., KURATSUJI, T. and KIRIKAE, T., 2005. Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6′)-Iae gene cassette encoding a novel aminoglycoside acetyltransferase. Antimicrobial Agents and Chemotherapy, vol. 49, no. 9, pp. 3734-3742. http://dx.doi.org/10.1128/AAC.49.9.3734-3742.2005. PMid:16127047. http://dx.doi.org/10.1128/AAC.49.9.3734-...
|
ParE R: TCG AGG GCG TAG TAG ATG TCC TTG CCG A |