Fungos endofíticos para o biocontrole de <i>Duponchelia fovealis</i> (Zeller) (Lepidoptera: Crambidae)

Amatuzzi, R. F.; Cardoso, N.; Poltronieri, A. S.; Poitevin, C. G.; Dalzoto, P.; Zawadeneak, M. A.; Pimentel, I. C.

doi:10.1590/1519-6984.166681

R. F. Amatuzzi ^**e-mail: rfamatuzzi@gmail.com

Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná – UFPR, Avenida Coronel Francisco H. dos Santos, 100, CP 19031, CEP 81531-980, Curitiba, PR, Brazil

N. Cardoso

Departamento de Matemática e Estatistica, Universidade Federal de Rondônia – UNIR, Rua Rio Amazonas, 351, Jardins dos Migrantes, CEP 76900-726, Ji-Paraná, RO, Brazil

A. S. Poltronieri

Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná – UFPR, Avenida Coronel Francisco H. dos Santos, 100, CP 19031, CEP 81531-980, Curitiba, PR, Brazil

C. G. Poitevin

Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná – UFPR, Avenida Coronel Francisco H. dos Santos, 100, CP 19031, CEP 81531-980, Curitiba, PR, Brazil

P. Dalzoto

Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná – UFPR, Avenida Coronel Francisco H. dos Santos, 100, CP 19031, CEP 81531-980, Curitiba, PR, Brazil

M. A. Zawadeneak

Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná – UFPR, Avenida Coronel Francisco H. dos Santos, 100, CP 19031, CEP 81531-980, Curitiba, PR, Brazil

I. C. Pimentel

Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná – UFPR, Avenida Coronel Francisco H. dos Santos, 100, CP 19031, CEP 81531-980, Curitiba, PR, Brazil

*e-mail: rfamatuzzi@gmail.com

Site	Location	Geographical Coordinates	Crop
A	São José dos Pinhais	25º 36’ 753” S and 49º 04’ 887” W	Organic
B	Pinhais	25°23’30” S and 49°07’30” W	Organic
C	São José dos Pinhais	25º 33’580” S and 49º 08’ 887” W	Conventional
D	São José dos Pinhais	25º 35’ 820”S and 49º 05’ 866” W	Conventional

Characteristic	Site A	Site B	Site C	Site D
N^o of samples	100	100	100	100
N^o of isolates	183 ^* * Comparison of means using the Tukey test at 0.05 probability. Same lowercase letters do not differ at 95% level; a	123 ^* * Comparison of means using the Tukey test at 0.05 probability. Same lowercase letters do not differ at 95% level; b	114 ^* * Comparison of means using the Tukey test at 0.05 probability. Same lowercase letters do not differ at 95% level; b	97 ^* * Comparison of means using the Tukey test at 0.05 probability. Same lowercase letters do not differ at 95% level; b
average deviation	1.146	0.941	0.829	0.926
Isolate prevalence (%) ^ Isolate prevalence (%) = (total number of leaf pieces yielding more than one isolate)/(total number of leaf pieces in that trial) x100.	45.8%	30.7%	28.5%	24.3%

Genera	Number of isolates	%
Trichoderma sp.	2	0.7
Fusarium spp.	2	0.7
Paecilomyces spp.	4	1.43
Nigrospora sp.	11	3.95
Ulocladium sp.	15	5.39
Diaporthe sp.	16	5.75
Phyllosticta sp.	16	5.75
Bipolaris sp.	17	6.11
Phoma sp.	18	6.47
Chaetomium sp.	18	6.47
Penicillium sp.	21	7.55
Mycelia sterilia	24	8.63
Aspergillus spp.	27	9.71
Alternaria spp.	39	14.02
Cladosporium spp.	48	17.26
^* * Non-sporulated monosporic colonies. Unidentified	239	-

Species	Strain	GenBank Number	Gene
Aspergillus terreus	CD72E1	KT427380 KT427386	β-Tubulin Calmodulin
Aspergillus sydowwi	G70	KT427381	β-Tubulin
Paecilomyces lilacinus	G41	KT427382	β-Tubulin
Paecilomyces lilacinus	G42	KT427383	β-Tubulin
Diaporthe endophytica	G13	KT427385 KP2164961	ITS Calmodulin
Cladosporium sphaerospermum	OB96E2	KP2164981	ITS
Cladosporium cladosporides	G28	KP2164971	ITS
Fusarium solani	CD34F1	KP2164951	ITS

Treatment	G41	G42
Nº of larvae	689	671
^a a LC50 values and their 95% confidence levels are expressed in conidia per milliliter. LC50	3.6×107	5.4×107
Slope(SE)	0.524 ± 0.081	0.422 ± 0.050
95% FL	1.2×107- 8.4×107	1.3×107 - 9.2×107
χ2 (df=4)	0.444	0.880

[1] *e-mail: rfamatuzzi@gmail.com

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Fungos endofíticos para o biocontrole de Duponchelia fovealis (Zeller) (Lepidoptera: Crambidae)

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