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Distribution of aflatoxins in contaminated corn fractions segregated by size

Distribuição da aflatoxina em frações de milho contaminado, segregadas por tamanho

The efficiency of segregation, by particle size, in reducing the aflatoxin content of corn lots under the Brazilian conditions was studied. The aflatoxin content, in the corn fractions obtained after sieving thirty contaminated samples, with approximately 3.0 kg each, was determined. The samples were passed through 4.5 mm round-hole sieves and the fractions with particles ³4.5 mm (fraction A) and <4.5 mm (fraction B) and then analyzed for aflatoxins by TLC. The results showed that the distribution of the aflatoxins among the fractions A and B varied from sample to sample. However, the fraction B presented higher content (6 to 1,422 µg/kg) of aflatoxins (P<5%), than samples of fraction A (4 to 389 µg/kg). In spite of that the segregation of this fraction did not reduce significantly the contamination of the samples. This probably occurred because the contribution of the fraction B for the estimated total of the concentration of aflatoxin of the sample was smaller than that of the fraction A, due to the smallest amount of the fraction B in the sample weight total. In spite of being significant for the studied samples the correlation among the percentages of the groups of grains with defects a) burning grains, sprouted, burned and moldy and b) total injured, with the estimated level of contamination for the samples, more studies will be necessary so that better conclusions can be drawn for this correlation.

aflatoxin; corn; contamination distribution; segregation; fractions


Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
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