Occurrence of genes encoding enterotoxins in uropathogenic   <i>Escherichia coli</i> isolates

Mirzarazi, Mahsa; Rezatofighi, Seyedeh Elham; Pourmahdi, Mahdi; Mohajeri, Mohamad Reza

doi:10.1590/S1517-838246120130860

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Brazilian Journal of Microbiology

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Medical Microbiology • Braz. J. Microbiol. 46 (1) • Jan-Mar 2015 • https://doi.org/10.1590/S1517-838246120130860 copy

Occurrence of genes encoding enterotoxins in uropathogenic Escherichia coli isolates

Authorship SCIMAGO INSTITUTIONS RANKINGS

To determine the presence of some toxins of diarrheagenic Escherichia coli (DEC) in uropathogenic E. coli (UPEC), 138 urinary tract infection (UTI)-causing UPECs were analyzed. The astA, set, sen and cdtB genes were detected in 13 (9.4%), 2 (1.3%), 13 (9.4%) and 0 (0%) of UPEC isolates respectively. The results show that some genes encoding toxins can be transferred from DEC pathotypes to UPECs therefore these isolates can transform into potential diarrhea-causing agents.

uropathogenic Escherichia coli; toxins; phylogenetic groups; set ; sen ; astA and cdt genes

Gene	Primer name	Sequence in 5′ → 3′ direction	Size of product (bp)	Reference
set-1B	ShET-1B upper	GTGAACCTGCTGCCGATATC	147	Fasano et al., 2011
	ShET-1B lower	ATTTGTGGATAAAAATGACG
Sen	ShET-2 upper	ATGTGCCTGCTATTATTTAT	799	*Nataro et al., 1995*Nataro JP, Seriwatana J, Fasano A et al. (1995) Identification and cloning of a novel plasmid-encoded enterotoxin of enteroinvasive Escherichia coli and Shigellastrains. Infect Immun 63:4721–4728.
	ShET-2 lower	CATAATAATAAGCGGTCAGC		*Vila et al., 2000*Vila J, Vargas M, Henderson IR et al. (2000) Enteroaggregative Escherichia coli virulence factors in traveler’s diarrhea strains. J Infect Dis 182:1780–1783.
astA	East-upper	ATGCCATCAACACAGTATAT	110	Johnson and Stell, 2000Johnson JR, Stell AL (2000) Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise. J Infect Dis 181:261–272.
	East-lowe	GCGAGTGACGGCTTTGTAGT
cdtB	cdt-a1	AAATCACCAAGAATCATCCAGTTA	430
	cdt-a2	AAATCTCCTGCAATCATCCAGTTTA		Chen and Griffiths, 1998Chen J, Griffiths MW (1998) PCR differentiation of Escherichia coli from other Gramnegative bacteria using primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein. Lett Appl Microbiol 27:369–371.
	cdt-s1	GAAAGTAAATGGAATATAAATGTCCG		Clermont et al., 2010
	cdt-s2	GAAAATAAATGGAACACACATGTCCG		Clermont et al., 2010
uspA	uspA-up	CCGATACGCTGCCAATCAGT	883	Clermont et al., 2010
	uspA-down	ACGCAGACCGTAGGCCAGAT
ChuA	ChuA.1	GACGAACCAACGGTCAGGA	279
	ChuA.2	TGCCGCCAGTACCAAAGACA
YjaA	YjaA.1	TGAAGTGTCAGGAGACGCTG	211
	YjaA.2	ATGGAGAATGCGTTCCTCAAC
TspE4C2	TspE4C2.1	GAGTAATGTCGGGGCATTCA	152
	TspE4C2.2	CGCGCCAACAAAGTATTACG

Gene	UPEC isolates	Phylogenetic groups of UPEC isolates				Commensal E. coli isolates	Phylogenetic groups of commensal E. coli isolates

		A	B1	B2	D		A	B1	B2	D
	138 (%)	17 (%)	16 (%)	76 (%)	29 (%)	30 (%)	9 (%)	0 (%)	9 (%)	12 (%)
set	2 (1.3)	0 (0)	0 (0)	2 (2.6)	0 (0)	0(0)	0 (0)	0 (0)	0 (0)	0 (0)
sen	13 (9.4)	1 (5.8)	2 (12.5)	4 (5.2)	6 (20.7)	2(6.6)	0 (0)	0 (0)	2 (22)	0 (0)
astA	13 (9.4)	1 (5.8)	2 (12.5)	9 (11.8)	1 (3.4)	5 (16.6)	0 (0)	0 (0)	4 (44)	1 (8)
cdtB	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
astA+sen	3 (2.2)	0 (0)	0 (0)	3 (3.9)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)

Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br

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[1] Send correspondence to S.E. Rezatofighi. Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, 6135743135 Ahvaz, Iran. E-mail: e.tofighi@scu.ac.ir.