Production and purification of an Endo–1,4-beta-Xylanase from Humicola grisea var. thermoidea by electroelution

Produção e purificação de uma Endo–1,4-beta-Xylanase de Humicola grisea var. thermoidea por eletroeluição

Humicola grisea var. thermoidea produces two forms of extracellular xylanase. The component form 1 was purified using the electroelution method, due to the very small production of this extracellular enzyme. The apparent molecular mass was 61.8 kDa by SDS-PAGE.

electroelution; enzyme purification; extracellular xylanase; Humicola grisea var. thermoidea