Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.
Bartonella; Culture Media; False Positive Reactions; Polymerase Chain Reaction; Sheep-Blood Contamination