Aspergillus sp. 13.33 |
SSF |
- |
45 °C |
844.6 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Aspergillus sp. 13.34 |
SSF |
- |
45 °C |
469.6 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Aspergillus sp. 13.35 |
SSF |
- |
45 °C |
640.5 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Aspergillus sp. |
SSF |
- |
30 °C |
107.66 |
- |
Soybean |
(Rajmalwar and Dabholkar, 2009Rajmalwar S, Dabholkar PS (2009) Production of protease by Aspergillus sp. using solidstate fermentation. Afr J Biotechnol 8:4197–4198.) |
Aspergillus awamori
|
SmF |
5.0 |
55 °C |
- |
Acid protease |
Wheat bran |
(Negi and Banerjee, 2009Negi S, Banerjee R (2009) Characterization of amylase and protease produced by Aspergillus awamori in a single bioreactor. Food Res Int 42:443–448.) |
Aspergillus clavatus ES1 |
SmF |
8.5 |
50 °C |
4970 |
Alkaline protease |
Wheat bran, fish flour |
(Hajji et al., 2007Hajji M, Kanoun S, Nasri M et al. (2007) Purification and characterization of an alkaline serine-protease produced by a new isolated Aspergillus clavatus ES1. Process Biochem 42:791–797.) |
Aspergillus flavus 1.2 |
SSF |
- |
45 °C |
117.6 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Aspergillus flavus
|
SSF |
7.5–9.5 |
32 °C |
6.8 |
Alkaline protease |
Wheat bran |
(Malathi and Chakraborty, 1991Malathi S, Chakraborty R (1991) Production of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent. Appl Environ Microbiol 57:712–716.) |
Aspergillus flavus
|
SSF |
7.5 |
45 °C |
640 |
Alkaline serine |
Wheat bran |
(Kranthi et al., 2012Kranthi VS, Rao DM, Jaganmohan P (2012) Production of Protease by Aspergillus flavus Through Solid State Fermentation Using Different Oil Seed Cakes. Int J Microbiol Res 3:12–15.) |
Aspergillus flavus
|
SSF |
7.0 |
36 °C |
1894 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Aspergillus fumigates
|
SSF |
8.0 |
50 °C |
- |
Serine protease |
Wheat bran |
(Silva et al., 2010Silva RR, Rodrigues A, Cabral H (2010) Investigation of Liquid and Solid Fermentation Processes of the Fungus Aspergillus fumigatus for Protease Production. J Biotechnol 150S:S1–S576.) |
Aspergillus niger
|
SmF |
4.0 |
30 °C |
200 |
Acid protease |
Rice |
(Yang and Lin, 1998Yang F, Lin I (1998) Production of acid protease using thin stillage from a rice-spirit distillery by Aspergillus niger. Enzym Microb Technol 23:397–402.) |
Aspergillus niger
|
SmF |
3.0 |
27 °C |
3600 |
- |
Yeast extract, malt extract, peptone and dextrose |
(O'Donnell et al., 2001O'Donnell D, Wang L, Xu J et al. (2001) Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity. Biochem Eng J 8:187–193.) |
Aspergillus oryzae IAM2704 |
SmF |
7.0 |
30 °C |
1550 |
Neutral protease |
Casein, glucose |
(Ogawa et al., 1995Ogawa A, Yasuhara A, Tanaka T et al. (1995) Production of Neutral Protease by Membrane-Surface of Aspergillus oryzae IAM2704. J Ferment Bioeng 80:35–40.) |
Aspergillus oryzae MTCC 5341 |
SSF |
3.0–4.0 |
55 °C |
43.658 |
Aspartate protease |
Wheat bran |
(Vishwanatha et al., 2009Vishwanatha KS, Rao AGA, Singh SA (2009) Characterisation of acid protease expressed from Aspergillus oryzae MTCC 5341. Food Chem 114:402–407.) |
Aspergillus oryzae MTCC 5341 |
SSF |
6.3 |
55 °C |
3500 |
Aspartate protease |
Wheat bran, Soy flour, Skim milk |
(Vishwanatha et al., 2010aVishwanatha KS, Appu Rao AG, Singh SA (2010a) Production and characterization of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341. Appl Microbiol Biotechnol 85:1849–1859.) |
Aspergillus oryzae NCIM 649 |
SSF |
7.0 |
36 °C |
6301 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Aspergillus oryzae NCIM 1212 |
SSF |
7.0 |
36 °C |
1631 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Aspergillus oryzae NCIM 1032 |
SSF |
7.0 |
36 °C |
4744 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Aspergillus parasiticus
|
SSF |
8.0 |
40 °C |
17.65 |
Serine protease |
Wheat bran |
(Tunga et al., 2003Tunga R, Shrivastava B, Banerjee R (2003) Purification and characterization of a protease from solid state cultures of Aspergillus parasiticus. Process Biochem 38:1553–1558.) |
Aspergillus parasiticus
|
SSF |
7.0 |
36 °C |
9545 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Aspergillus ustus
|
SmF |
9.0 |
45 °C |
1639 |
Serine protease |
Skim milk |
(Damare et al., 2006Damare S, Raghukumar C, Muraleedharan UD et al. (2006) Deep-sea fungi as a source of alkaline and cold-tolerant proteases. Enzym Microb Technol 39:172–181.) |
Beauveria sp. MTCC 5184 |
SmF |
6.5–7.5 |
28 °C |
9783 |
Serine protease |
Mustard seed cake |
(Shankar et al., 2011Shankar S, Rao M, Laxman RS (2011) Purification and characterization of an alkaline protease by a new strain of Beauveria sp. Process Biochem 46:579–585.) |
Beauveria bassiana
|
SmF |
7.0 |
26 °C |
280.72 |
Alkaline protease |
Shrimp shell, soy powder |
(Rao et al., 2006Rao YK, Lu S-C, Liu B-L et al. (2006) Enhanced production of an extracellular protease from Beauveria bassiana by optimization of cultivation processes. Biochem Eng J 28:57–66.) |
Beauveria felina
|
SSF |
7.0 |
28 °C |
8211 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Botrytis cinérea
|
SmF |
8.0 |
50 °C |
388 |
Alkaline protease |
Spirulina algae |
(Abidi et al., 2011Abidi F, Chobert J-M, Haertlé T et al. (2011) Purification and biochemical characterization of stable alkaline protease Prot-2 from Botrytis cinerea. Process Biochem 46:2301–2310.) |
Clonostachys rosea
|
SmF |
9.0 – 10.0 |
60 °C |
149.3 |
Serine protease |
Glucose, gelatin, peptone, yeast extract |
(Li et al., 2006Li J, Yang J, Huang X et al. (2006) Purification and characterization of an extracellular serine protease from Clonostachys rosea and its potential as a pathogenic factor. Process Biochem 41:925–929.) |
Conidiobolus coronatus
|
SmF |
6.0 – 7.0 |
28 °C |
72.46 |
Alkaline protease |
Malt extract, glucose, yeast extract, petone |
(Laxman et al., 2005Laxman RS, Sonawane AP, More SV et al. (2005) Optimization and scale up of production of alkaline protease from Conidiobolus coronatus. Process Biochem 40:3152–3158.) |
Cordyceps militaris
|
SmF |
8.5 – 12.0 |
25 °C |
818.7 |
Trypsin-like protease |
Colloidal chitin |
(Hattori et al., 2005Hattori M, Isomura S, Yokoyama E et al. (2005) Extracellular trypsin-like proteases produced by Cordyceps militaris. J Biosci Bioeng 100:631–636.) |
Cordyceps sinensis
|
SmF |
7.0 |
40–55 °C |
- |
Chymotrypsin-like serine |
Sucrose, mannose, galactose, tryptone, yeast extract |
(Zhang et al., 2008Zhang Y, Liu X, Wang M (2008) Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic fungus Cordyceps sinensis. Res Microbiol 159:462–469.) |
Engyodontium album
|
SSF |
10.0 – 11.0 |
45–60 °C |
3186 |
Alkaline protease |
Wheat bran |
(Chellappan et al., 2006Chellappan S, Jasmin C, Basheer SM et al. (2006) Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation. Process Biochem 41:956–961.) |
Fusurium oxysporum
|
SmF |
8.0 |
45 °C |
492 |
Trypsin-like protease |
Gelatin |
(Barata et al., 2002Barata RA, Andrade MHG, Rodrigues RD et al. (2002) Purification and Characterization of an Extracellular Trypsin-Like Protease of Fusarium oxysporum var. lini. J Biosci Bioeng 94:304–308.) |
Graphium putredinis
|
- |
7.0 |
50 °C |
114 |
Serine protease |
Soya bean meal |
(Savitha et al., 2011Savitha S, Sadhasivam S, Swaminathan K et al. (2011) Fungal protease: Production, purification and compatibility with laundry detergents and their wash performance. J Taiwan Inst Chem Eng 42:298–304.) |
Hirsutella rhossiliensis
|
SmF |
7.0 |
40 °C |
7.7 |
Serine protease |
Panagrellus redivivus |
(Wang et al., 2007Wang B, Wu W, Liu X (2007) Purification and characterization of a neutral serine protease with nematicidal activity from Hirsutella rhossiliensis. Mycopathol 163:169–176.) |
Humicola lutea 120-5 |
SmF |
3.0 – 3.5 |
28 °C |
1100 |
Acid proteinase |
Glucose, casein |
(Aleksieva and Peeva, 2000Aleksieva P, Peeva L (2000) Investigation of acid proteinase biosynthesis by the fungus Humicola lutea 120–5 in an airlift bioreactor. Enzym Microb Technol 26:402–405.) |
Metarhizium anisopliae
|
SmF |
5.5 |
28 °C |
1.12 |
- |
Czapek-Dox |
(Kucera, 1981Kucera M (1981) The production of toxic protease by the entomopathogenous fungus Metarhizium anisopliae in submerged culture. J Invertebr Pathol 38:33–38.) |
Metarhizium anisopliae MTCC 892 |
SSF |
7.0 |
28 °C |
6452 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Mucor circinelloides
|
SmF |
5.2 |
25 °C |
25 |
- |
Glucose |
(Andrade et al., 2002Andrade VS, Sarubbo LA, Fukushima K et al. (2002) Production of extracellular proteases by Mucor circinelloides using D-glucose as carbon source / substrate. Braz J Microbiol 33:106–110.) |
Myceliophthora sp. |
SmF/SSF |
7.0/9.0 |
50 °C |
1.78 |
Alkaline protease |
Casein, wheat bran |
(Zanphorlin et al., 2010Zanphorlin LM, Facchini FD, Vasconcelos F et al. (2010) Production, partial characterization, and immobilization in alginate beads of an alkaline protease from a new thermophilic fungus Myceliophthora sp. J Microbiol 48:331–336.) |
Ophiostoma piceae 387N |
- |
7.0 – 9.0 |
40 °C |
56.1 |
Subtilisin serine proteinase |
Soybean |
(Abraham and Breuil, 1996Abraham LD, Breuil C (1996) Isolation and characterization of a subtilisin-like serine proteinase secreted by the sap-staining fungus Ophiostoma piceae. Enzym Microb Technol 18:133–140.) |
Penicillium sp. |
SSF |
6.0 – 9.0 |
45 °C |
51.6 |
Serine protease |
Defatted soybean |
(Germano et al., 2003Germano S, Pandey A, Osaku CA et al. (2003) Characterization and stability of proteases from Penicillium sp. produced by solid-state fermentation. Enzym Microb Technol 32:246–251.) |
Penicillium sp. |
SSF |
7.0 |
36 °C |
4819 |
Alkaline protease |
Wheat bran, soy protein |
(Agrawal et al., 2005Agrawal D, Patidar P, Banerjee T et al. (2005) Alkaline protease production by a soil isolate of Beauveria felina under SSF condition: parameter optimization and application to soy protein hydrolysis. Process Biochem 40:1131–1136.) |
Penicillium camemberti
|
SmF |
3.4 |
50 °C |
1276 |
Acid proteinase |
- |
(Chrzanowska et al., 1995Chrzanowska J, Kolaczkowska M, Dryjanski M et al. (1995) Aspartic proteinase from Penicillium camemberti: purification, properties, and substrate specificity. Enzym Microb Technol 17:719–724.) |
Penicillium citrinum
|
- |
7.0 |
40 °C |
- |
Serine proteinase |
- |
(Yamamoto et al., 1993Yamamoto N, Matsumoto K, Yamagata Y et al. (1993) A heat-labile serine proteinase from Penicillium citrinum. Phytochem 32:1393–1397.) |
Penicillium griseoroseum IH-02 |
SSF |
5.0 |
30 °C |
8.2 |
Acid protease |
Soybean meal, wheat bran |
(Ikram-Ul-Haq and Mukhtar, 2007Ikram-Ul-Haq, Mukhtar H (2007) Biosynthesis of acid proteases by Penicillium griseoroseum IH-02 in solid-state fermentation. Pak J Bot 39:2717–2724.) |
Penicillium restrictum
|
SSF |
7.0 – 8.0 |
37 °C |
7.9 |
- |
Starch |
(Gombert et al., 1999Gombert AK, Pinto AL, Castilho LR et al. (1999) Lipase production by Penicillium restrictum in solid-state fermentation using babassu oil cake as substrate. Process Biochem 35:85–90.) |
Penicillium roqueforti
|
- |
5.0 |
25 °C |
1394.7 |
Acid protease |
Czapek-Dox, peptone |
(Larsen et al., 1998Larsen MD, Kristiansen KR, Hansen TK (1998) Characterization of the proteolytic activity of starter cultures of Penicillium roqueforti for production of blue veined cheeses. Int J Food Microbiol 43:215–221.) |
Phanerochaete chrysosporium
|
SSF |
4.5 |
25 °C |
35 |
Acid and thiolproteases |
Glucose |
(Cabaleiro et al., 2002Cabaleiro DR, Rodríguez-Couto S, Sanromán A et al. (2002) Comparison between the protease production ability of ligninolytic fungi cultivated in solid state media. Process Biochem 37: 1017–1023.) |
Phanerochaete chrysosporium
|
SmF |
6.5 |
37 °C |
231–290 |
- |
Glucose |
(Xiaoping et al., 2008Xiaoping X, Xianghua W, Yanan B et al. (2008) Effects of culture conditions on ligninolytic enzymes and protease production by Phanerochaete chrysosporium in air. J Environ Sci 20:94–100.) |
Phanerochaete radiata
|
SSF |
4.5 |
25 °C |
50 |
Thiolproteases |
Glucose |
(Cabaleiro et al., 2002Cabaleiro DR, Rodríguez-Couto S, Sanromán A et al. (2002) Comparison between the protease production ability of ligninolytic fungi cultivated in solid state media. Process Biochem 37: 1017–1023.) |
Rhizomucor sp. |
SSF |
- |
45 °C |
770.9 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Rhizopus SMC |
SmF |
4.3 |
28 °C |
2000 |
Acid protease |
Wheat bran, casein |
(Ramamurthy et al., 1991Ramamurthy V, Upadhyay CM, Kothari RM (1991) An optimized protocol for the preparation and application of acid protease. J Biotechnol 21:187–196.) |
Rhizopus oryzae
|
SSF |
5.5 |
60 °C |
760 |
Aspartate protease |
Wheat bran |
(Kumar et al., 2005Kumar S, Sharma NS, Saharan MR et al. (2005) Extracellular acid protease from Rhizopus oryzae: purification and characterization. Process Biochem 40:1701–1705.) |
Thermoascus aurantiacus
|
SSF |
- |
45 °C |
258.3 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Thermoascus aurantiacus
|
SSF |
5.5 |
60 °C |
248 |
Acid protease |
Wheat bran |
(Merheb et al., 2007Merheb CW, Cabral H, Gomes E et al. (2007) Partial characterization of protease from a thermophilic fungus, Thermoascus aurantiacus, and its hydrolytic activity on bovine casein. Food Chem 104:127–131.) |
Thermomucor indicae-seudaticae N31 |
SSF |
5.7 |
70 °C |
160 |
Acid protease |
Wheat bran |
(Merheb-Dini et al., 2010Merheb-Dini C, Gomes E, Boscolo M et al. (2010) Production and characterization of a milk-clotting protease in the crude enzymatic extract from the newly isolated Thermomucor indicae-seudaticae N31 (Milk-clotting protease from the newly isolated Thermomucor indicae-seudaticae N31). Food Chem 120:87–93.) |
Thermomyces lanuginosus
|
SmF |
6.0 |
50 °C |
0.71 |
- |
Glucose, citric acid |
(Jensen et al., 2002Jensen B, Nebelong P, Olsen J et al. (2002) Enzymes production in continuous cultivation by the thermophilic fungus, Thermomyces lanuginosus. Biotechnol Lett 24:41–45.) |
Thermomyces lanuginosus
|
SSF |
- |
45 °C |
945.2 |
- |
Wheat bran |
(Macchione et al., 2008Macchione MM, Merheb CW, Gomes E et al. (2008) Protease production by different thermophilic fungi. Appl Biochem Biotechnol 146:223–230.) |
Thermomyces lanuginosus
|
SmF |
5.0 |
70 °C |
12.8 |
Serine protease |
Casein, glucose, yeast extract |
(Li et al., 1997Li D, Yang Y, Shen C (1997) Protease production by the thermophilic fungus Thermomyces lanuginosus. Mycol Res 101:18–22.) |
Trichoderma harzianum
|
- |
8.0 |
60 °C |
99 |
Serine protease |
Soya bean meal |
(Savitha et al., 2011Savitha S, Sadhasivam S, Swaminathan K et al. (2011) Fungal protease: Production, purification and compatibility with laundry detergents and their wash performance. J Taiwan Inst Chem Eng 42:298–304.) |
Trichoderma reesei QM9414 |
- |
8.0 |
50 °C |
0.25 |
Trypsin serine proteases |
Glucose |
(Dienes et al., 2007Dienes D, Börjesson J, Hägglund P et al. (2007) Identification of a trypsin-like serine protease from Trichoderma reesei QM9414. Enzym Microb Technol 40:1087–1094.) |