ica A (1315 bp) |
CCT AAC TAA CGA AAG GTA G AAG ATA TAG CGA TAA GTG C |
(92 ºC 45 s, 49 ºC 45 s, 72 ºC 1 min) × 30 and 72 ºC 7 min |
Vasudevan P et al.2222 Vasudevan P, Nair MKM, Annamalai T, Venkitanarayana KS. Phenotypic and Genotipic characterization of bovine mastitis isolates S. aureus for biofilm formation. Vet Microbiol. 2003;92:179-185.
|
ica D (381 bp) |
AAA CGT AAG AGA GGT GG GGC AAT ATG ATC AAG ATA C |
(92 ºC 45 s, 49 ºC 45 s, 72 ºC 1 min) × 30 and 72 ºC 7 min |
Vasudevan P et al.2222 Vasudevan P, Nair MKM, Annamalai T, Venkitanarayana KS. Phenotypic and Genotipic characterization of bovine mastitis isolates S. aureus for biofilm formation. Vet Microbiol. 2003;92:179-185.
|
bap (971 bp) |
CCC TAT ATC GAA GGT GTA GAA TTG GCT GTT GAA GTT AAT ACT GTA CCT GC |
94 ºC 2 min (94 ºC 30 s, 55 ºC 30 s, 72 ºC 75 s) × 40 and 72 ºC 5 min |
Cucarella C et al.88 Cucarella C, Tormo MA, Úbeda C, et al. Role of biofilm-associated protein Bap in the pathogenesis of bovine S. aureus. Infect Immun. 2004;72(4):2177-2185.
|
agr RNAIII (200 bp) |
CAT AGC ACT GAG TCC AAG GA CAA TCG GTG ACT TAG TAA AAT G |
94 ºC 3 min (94 ºC 1 min, 55 ºC 1 min, 72 ºC 1 min) × 30 and 72 ºC 5 min |
Reinoso EB2323 Reinoso EB (Tese de Doutorado) Análisis epidemiológico y molecular de cepas de S. aureus de distintosorígenes. Rio Cuarto, Argentina: Instituto de Microbiologia, UNRC; 2004, 199 pp.
|
agr I (440 bp) |
ATG CAC ATG GTG CAC ATG C GTC ACA AGT ACT ATA AGC TGC GAT |
(94 ºC 1 min, 55 ºC 1 min, 72 ºC 1 min) × 25 |
Shopsin B et al.77 Shopsin B, Mathema B, Alcabes P, et al. Prevalence of agr specificity groups among S. aureus strains colonizing children and their guardians. J Clin Microbiol. 2003;41(1):456-459.
|
agr II (572 bp) |
ATG CAC ATG GTG CAC ATG C GTA TTA CTA ATT GAA AAG TGC CAT AGC |
(94 ºC 1 min, 55 ºC 1 min, 72 ºC 1 min) × 25 |
Shopsin B et al.77 Shopsin B, Mathema B, Alcabes P, et al. Prevalence of agr specificity groups among S. aureus strains colonizing children and their guardians. J Clin Microbiol. 2003;41(1):456-459.
|
agr III (406 bp) |
ATGCACATGGTGCACATGC CTGTTGAAAAAGTCAACTAAAAGCTC |
(94 ºC 1 min, 55 ºC 1 min, 72 ºC 1 min) × 25 |
Shopsin B et al.77 Shopsin B, Mathema B, Alcabes P, et al. Prevalence of agr specificity groups among S. aureus strains colonizing children and their guardians. J Clin Microbiol. 2003;41(1):456-459.
|
agr IV (588 bp) |
ATGCACATGGTGCACATGC CGATAATGCCGTAATACCCG |
(94 ºC 1 min, 55 ºC 1 min, 72 ºC 1 min) × 25 |
Shopsin B et al.77 Shopsin B, Mathema B, Alcabes P, et al. Prevalence of agr specificity groups among S. aureus strains colonizing children and their guardians. J Clin Microbiol. 2003;41(1):456-459.
|
mec A (533 bp) |
AAA ATC GAT GGT AAA GGT TGG C AGT TCT GCA GTA CCG GAT TTG C |
94 ºC 4 min (94 ºC 30 s, 53 ºC 30 s, 72 ºC 1 min) × 30 and 72 ºC 4 min |
Murakami KW et al.2424 Murakami KW, Minamide K, Wada W, Nakamura E, Teraoka H, Watanbe S. Identification of methicillin resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol. 1991;29:2240-2244.
|
mecA (574 bp) |
ACG TTA CAA GAT ATG AAG ACA TTA ATA GCC ATC ATC |
95 ºC 5 min (94 ºC 1 min, 55 ºC 1 min, 72 ºC 1 min) × 30 and 72 ºC 10 min |
Melo DA et al.2525 Melo DA, Coelho IS, Motta CC, et al. Impairments of mecA gene detection in bovine Staphylococcus spp.. Braz J Microbiol. 2014;45(3):1075-1082.
|
bla Z (861 bp) |
TAC AAC TGT AAT ATC GGA GG CAT TAC ACT CTT GGC GGT TT |
94 ºC 5 min (94 ºC 30 s, 58 ºC 30 s, 72 ºC 30 s) × 35 and 72 ºC 5 min |
Rosato AE et al.2626 Rosato AE, Kreiswirth BN, Graig WA, Eisner W, Climo MW, Aecher GL. mecA-blaZ corepressors in clinical S. aureus isolates. Antimicrob Agents Chemother. 2003;47:1463-1466.
|