Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus

Infecção aguda e latente em camundongos expostos a uma amostra virulenta do vírus da doença de Aujeszky

Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.

Aujeszky’s disease virus; polymerase chain reaction; dot-blot hybridization