Trichosporon pullulans (Lidner) Diddens & Lodder isolated from the oral cavity of AIDS patient

Abstract

Trichosporon has been reported as a remarkable emerging pathogen and usually occurs in imunodrepressed patients. An oropharyngeal secretion sample was collected from a patient with AIDS, at the Sector of Infectious and Parasitic Diseases, Osvaldo Cruz University Hospital, Pernambuco State, Brazil. No yeast cells or arthroconidia were observed in the direct exam, but a culture was positive for Trichosporon pullulans, which is being reported in an AIDS patient for the first time.

Trichosporon pullulans; AIDS


Rejane Pereira Neves; Maria Auxiliadora de Queiroz Cavalcanti; Guilherme Maranhão Chaves; Oliane Maria Correia Magalhães

Departamento de Micologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil

Correspondence

SHORT COMMUNICATION

ABSTRACT

Trichosporon has been reported as a remarkable emerging pathogen and usually occurs in imunodrepressed patients. An oropharyngeal secretion sample was collected from a patient with AIDS, at the Sector of Infectious and Parasitic Diseases, Osvaldo Cruz University Hospital, Pernambuco State, Brazil. No yeast cells or arthroconidia were observed in the direct exam, but a culture was positive for Trichosporon pullulans, which is being reported in an AIDS patient for the first time.

Key words: Trichosporon pullulans, AIDS

The importance of the study of fungi as pathogenic agents from the oral cavity has been reported, indicating the mouth as an entrance of many mycosis etiological agents (11,14,15). Trichosporon is found in the indigenous mycobiota of the skin surface, mainly in the genito crural and perianal areas of homosexuals (17).

Hematogenic dissemination due to Trichosporon species is frequently observed in patients with low immunity, particularly in cases of neutropenia or leukemia (6,10,16). Among the species connected to mycosis, T. pullulans is recognized as usually occurring in imunodepressed individuals (2,6,8,9,12).

This study reports the isolation of T. pullulans from a clinical sample of an AIDS patient, hospitalized in the Sector of Infectious and Parasitic Diseases, University Hospital Osvaldo Cruz, Pernambuco State University, Recife, Pernambuco.

The patient is a 35-years-old male (C.J.V.), living as a mason in Paulista (Recife Metropolitan Region – Pernambuco State, Brazil), and presented lesions in the oral cavity and underside of the tongue. The sample of oropharyngeal secretion was collected with a sterilized swab that was placed into a tube containing 2mL of sterile destilled water plus 50 mg of chloranphenicol/L. At the Medical Mycology Laboratory (Department of Mycology, Federal University of Pernambuco), the sample was processed for direct examination and isolation in culture. The time from collecting to processing the sample did not exceed two hours.

The direct examination was performed on the fresh sample (without clarification and staining) and for the isolation, it spreading was performed on the surface of Sabouraud agar with 0.5 mg of yeast extract/L and 50 mg chloranphenicol/L was placed and incubated at room temperature (28º C ± 2º C) for 10 days. After the isolation, a suspension using sterile distilled water plus 50 mg of chloranphenicol/L, was prepared and 0.2 mL were placed on the surface of Sabouraud agar into tube. For identification, the morphophysiological and biochemical tests such as auxanogram, zimogram, acid and urease production were performed according to Barnett et al. (1), Kreger Van-Rij (7) and Lodder (13). The identification was confirmed by the VITEK 120 test.

No like-fungi structure was observed in the direct examination. Macroscopically the colony was cream, smooth, firm texture with irregular edges. In Sabouraud broth sediment was observed, but not ring or pellicle. Microscopically ovoid yeast cells, true mycelium and arthrospores were observed. Glucose, galactose, sucrose, lactose, trehalose, cellobiose, melezitose, raffinose, xylose, maltose and ammonium sulfate were assimilated as carbon and nitrogen sources. There was no fermentation or acid production and it was urease positive.

According to Barnett et al. (1) T. pullulans has been found in wood pulp, soil, pipelines in a brewery, butter, frozen beef and human nail, but no report of this species from the clinical samples of AIDS patients was found. On the other hand, T. pullulan has also been isolated from bovine mastitis (3), pigeon droppings (4), nasal infection in cats (5), cancer patients using (8) or not catheters (9), and leukemic patient associated with lung infection (16).

Hence, it is the first time that T. pullulans has been reported as isolated from AIDS patient.

ACKNOWLEDGMENT

Authors thanks to CAPES for financial support (scholarships).

Submitted: January 30, 2002; Returned to authors for corrections: June 18, 2002; Approved: September 24, 2002

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    (Lidner) diddens & lodder isolated from the oral cavity of AIDS patient
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Publication Dates

  • Publication in this collection
    23 June 2003
  • Date of issue
    Sept 2002

History

  • Accepted
    24 Sept 2002
  • Reviewed
    18 June 2002
  • Received
    30 Jan 2002
Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
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