The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
Keywords:
Enzymes; Cellulases; Fungus; Hydrolysis
| Fungi | Celullase total (FPase) (U g-1) | AVICELase (U g-1) | CMCase (U g-1) | β-Glucosidase (U g-1) | Xylanase (U g-1) | Laccase (U g-1) | Protein mg g-1 colonized substrate |
|---|---|---|---|---|---|---|---|
| P. sanguineus PYC02 | 0.80 ± 0.05 | 0.88 ± 0.08 | 2.03 ± 0.18 | 0.38 ± 0.03 | 8.43 ± 0.00 | 14.33 ± 0.57 | 1.33 ± 0.13 |
| P. ostreatus PLO06 | 1.32 ± 0.03 | 4.12 ± 0.02 | 2.25 ± 0.07 | 0.18 ± 0.01 | 1.33 ± 0.00 | 5.65 ± 0.42 | 1.67 ± 0.14 |
| P. eryngii PLE04 | 0.65 ± 0.01 | 3.12 ± 0.02 | 1.43 ± 0.05 | 0.23 ± 0.03 | 1.80 ± 0.10 | 6.58 ± 0.68 | 2.30 ± 0.20 |
| P.chrysosporium PC | 1.13 ± 0.04 | 0.65 ± 0.04 | 4.03 ± 0.10 | 1.08 ± 0.04 | 2.37 ± 0.15 | 0.70 ± 0.08 | 1.96 ± 0.10 |
| T. versicolor TRAM01 | 1.02 ± 0.05 | 1.43 ± 0.07 | 2.25 ± 0.10 | 1.88 ± 0.05 | 8.03 ± 0.01 | 9.87 ± 0.53 | 1.65 ± 0.12 |
| Activities of crude extracts | Apparent optimal parameter | Fungi | ||||
|---|---|---|---|---|---|---|
| PC | PYC | TRAM01 | PLE04 | PLO06 | ||
| Cellulase total (FPAse) | pH | 5.0 | 5.0 | 4.0 | 7.0 | 7.0 |
| Temperature | 60 ºC | 70 ºC | 50 ºC | 50 ºC | 60 ºC | |
| Thermostabilitya | 40% | 35% | 35% | 20% | 10% | |
| CMCase | pH | 4.0 | 4.0 | 4.0 | 7.0 | 5.0 |
| Temperature | 50 ºC | 50 ºC | 50 ºC | 50 ºC | 60 ºC | |
| Thermostabilitya | 30% | 50% | 60% | 0% | 0% | |
| Avicelase | pH | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 |
| Temperature | 50 ºC | 50 ºC | 50 ºC | 50 ºC | 50 ºC | |
| Thermostabilitya | 20% | 0% | 25% | 0% | 0% | |
| β-Glucosidase | pH | 7.0 | 7.0 | 5.0 | 5.0 | 5.0 |
| Temperature | 50 ºC | 70 ºC | 60 ºC | 40 ºC | 40 ºC | |
| Thermostabilitya | 40% | 40% | 50% | 80% | 85% | |
