Abstract
Human plasma kallikrein, a serine proteinase, plays a key role in intrinsic blood clotting, in the kallikrein-kinin system, and in fibrinolysis. The proteolytic enzymes involved in these processes are usually controlled by specific inhibitors and may be influenced by several factors including glycosaminoglycans, as recently demonstrated by our group. The aim of the present study was to investigate the effect of glycosaminoglycans (30 to 250 µg/ml) on kallikrein activity on plasminogen and factor XII and on the inhibition of kallikrein by the plasma proteins C1-inhibitor and antithrombin. Almost all available glycosaminoglycans (heparin, heparan sulfate, bovine and tuna dermatan sulfate, chondroitin 4- and 6-sulfates) reduced (1.2 to 3.0 times) the catalytic efficiency of kallikrein (in a nanomolar range) on the hydrolysis of plasminogen (0.3 to 1.8 µM) and increased (1.9 to 7.7 times) the enzyme efficiency in factor XII (0.1 to 10 µM) activation. On the other hand, heparin, heparan sulfate, and bovine and tuna dermatan sulfate improved (1.2 to 3.4 times) kallikrein inhibition by antithrombin (1.4 µM), while chondroitin 4- and 6-sulfates reduced it (1.3 times). Heparin and heparan sulfate increased (1.4 times) the enzyme inhibition by the C1-inhibitor (150 nM).
Glycosaminoglycans; Human plasma kallikrein; Plasminogen; Factor XII; C1-inhibitor; Antithrombin
Braz J Med Biol Res, August 2003, Volume 36(8) 1055-1059
Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors
A.J. Gozzo1, V.A. Nunes1, H.B. Nader1, C.P. Dietrich1, A.K. Carmona2, M.U. Sampaio1, C.A.M. Sampaio1 and M.S. Araújo1
Departamentos de 1Bioquímica and 2Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
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References
Correspondence and Footnotes Correspondence and Footnotes Correspondence and Footnotes
Abstract
Human plasma kallikrein, a serine proteinase, plays a key role in intrinsic blood clotting, in the kallikrein-kinin system, and in fibrinolysis. The proteolytic enzymes involved in these processes are usually controlled by specific inhibitors and may be influenced by several factors including glycosaminoglycans, as recently demonstrated by our group. The aim of the present study was to investigate the effect of glycosaminoglycans (30 to 250 µg/ml) on kallikrein activity on plasminogen and factor XII and on the inhibition of kallikrein by the plasma proteins C1-inhibitor and antithrombin. Almost all available glycosaminoglycans (heparin, heparan sulfate, bovine and tuna dermatan sulfate, chondroitin 4- and 6-sulfates) reduced (1.2 to 3.0 times) the catalytic efficiency of kallikrein (in a nanomolar range) on the hydrolysis of plasminogen (0.3 to 1.8 µM) and increased (1.9 to 7.7 times) the enzyme efficiency in factor XII (0.1 to 10 µM) activation. On the other hand, heparin, heparan sulfate, and bovine and tuna dermatan sulfate improved (1.2 to 3.4 times) kallikrein inhibition by antithrombin (1.4 µM), while chondroitin 4- and 6-sulfates reduced it (1.3 times). Heparin and heparan sulfate increased (1.4 times) the enzyme inhibition by the C1-inhibitor (150 nM).
Key words: Glycosaminoglycans, Human plasma kallikrein, Plasminogen, Factor XII, C1-inhibitor, Antithrombin
Plasma prekallikrein is a protein that circulates in blood as the zymogen of human plasma kallikrein (huPK). Kallikrein may be involved in several physiological pathways such as activation of factor XII (1) and plasminogen by direct cleavage of the zymogen (2) or by activation of pro-urokinase (3).
The participation of huPK in physiological processes requires the existence of several controlling factors. Specific proteins have been found to inhibit huPK activity. On the other hand, biological compounds in contact with circulating proteinases such as plasma kallikrein are thought to affect their activity (4,5). The glycosaminoglycans (GAGs) are macromolecules present in blood vessels, which may interfere with kallikrein activity or inhibition (6,7). Recently we demonstrated that GAGs affect the action of huPK on kininogen hydrolysis and inflammation (8). In the present study, we report on the influence of some GAGs on other processes in which huPK participates such as plasminogen and factor XII activation, and on huPK inactivation by the physiological inhibitors C1-inhibitor and antithrombin.
The effect of GAGs on plasminogen activation by huPK was studied by determining plasmin activity on a synthetic substrate. Briefly, 3.1 nM huPK (8) in 50 mM tris(hydroxymethyl)aminomethane (Tris) buffer, pH 7.4, 12 mM NaCl, with or without 30 µg/ml GAGs (9), was incubated with plasminogen (0.3 to 1.8 µM) for 2 h at 37ºC as described (2). The chromogenic peptide H-D-valyl-leucyl-lysine p-nitroanilide (H-D-Val-Leu-Lys-pNan, 0.56 mM) was used to monitor plasmin activity. Plasmin generation corresponds to hydrolyzed plasminogen and its concentration was determined by comparison to the initial velocities of reaction using commercial plasmin as standard (data not shown). The catalytic parameters (KM and kcat) calculated for plasminogen hydrolysis in the absence of any GAGs agreed with those reported by others (2). Bovine dermatan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate, tuna dermatan sulfate and heparan sulfate reduced the catalytic efficiency of huPK from 63 to 16%, while heparin was the only GAG that increased this hydrolysis (44%), as shown in Table 1.
Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors. A.J. Gozzo, V.A. Nunes, H.B. Nader, C.P. Dietrich, A.K. Carmona, M.U. Sampaio, C.A.M. Sampaio and M.S. Araújo. Brazilian Journal of Medical and Biological Research, 36 (8): 1055, 2003.
The contact activation system of human plasma forms a complex surface involving factor XII, prekallikrein and high molecular weight kininogen. In this complex, prekallikrein is activated to kallikrein, which activates factor XII. The active form of factor XII (factor XIIa) anchored to high molecular weight kininogen is able to activate more prekallikrein in an autocatalytic cycle. Since factor XII is a natural substrate for huPK, we studied the influence of GAGs on this interaction. Human plasma kallikrein (10 nM) in 50 mM Tris buffer, pH 7.4, 12 mM NaCl, 1 mg/ml bovine serum albumin (BSA), with or without 100 µg/ml GAGs, was incubated for 20 min at 37ºC with factor XII (0.1 to 10 µM). H-D-prolyl-phenylalanyl-arginine p-nitroanilide (H-D-Pro-Phe-Arg-pNan, 0.4 mM) with 0.2 µM soybean trypsin inhibitor (SBTI), that completely inhibited kallikrein, and 10 µg/ml Polybrene that prevented continued factor XII activation, were added and the activity of formed factor XIIa was evaluated by hydrolysis of chromogenic peptide by measuring absorbance at 405 nm (10). The concentration of formed factor XIIa, and hence of hydrolyzed factor XII, was determined by comparison to the velocities of reaction of known concentrations of factor XIIa. The KM and kcat values for the hydrolysis of factor XII by huPK in the absence of GAGs were determined to be 2.36 µM and 1.00 x 10-3 s-1, respectively, as shown in Table 1. Heparin, tuna dermatan sulfate, bovine dermatan sulfate, chondroitin 6-sulfate, chondroitin 4-sulfate and heparan sulfate markedly increased (1.9 to 7.7 times) the catalytic efficiency of the enzyme on factor XII activation.
In order to study the effect of GAGs on huPK inhibition, a kinetic assay was performed based on a method described previously (6,11). The proteinase inhibitors C1-inhibitor or antithrombin (12) with or without GAGs were preincubated in 50 mM Tris buffer, pH 8.0, 150 mM NaCl at 37ºC for 5 min. After this period, pre-warmed huPK at final concentrations of 72-86 nM was added to the reactions, and 50-µl aliquots were removed at various times (0 to 20 min). Residual enzyme activity was assessed on the hydrolysis of the chromogenic substrate N-a-acetyl-phenylalanyl-arginine p-nitroanilide (Ac-Phe-Arg-pNan, 0.7 mM), monitored at 405 nm. The kinetics of huPK inhibition was studied with the inhibitors in 2- to 65-fold molar excess and each rate constant was determined twice. The straight lines obtained when natural logarithm of huPK residual activity was plotted versus time indicate a pseudo-first-order kinetic. The values of the apparent first-order rate constants (k) were calculated from the slopes of those lines and were found to be directly proportional to the inhibitor concentrations - [I] - (data not shown). The second-order rate constants (k") were calculated from the equation k = k" x [I].
The data showed a second-order rate constant of 240 M-1 s-1 for kallikrein inhibition by antithrombin, in agreement with a previous study (4); this rate, however, increased about 3.4-fold in the presence of heparin (Table 2). In addition, it was demonstrated that antithrombin-mediated huPK inhibition is enhanced by heparan sulfate and bovine and tuna dermatan sulfate, and is impaired by chondroitin 4- and 6-sulfates. Kallikrein inhibition by C1-inhibitor was slightly increased in the presence of GAGs (Table 2) according to the calculated apparent second-order rate constants.
Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors. A.J. Gozzo, V.A. Nunes, H.B. Nader, C.P. Dietrich, A.K. Carmona, M.U. Sampaio, C.A.M. Sampaio and M.S. Araújo. Brazilian Journal of Medical and Biological Research, 36 (8): 1055, 2003.
The relationship between increased iduronic acid and sulfate contents and the enhancement of anticoagulant and antithrombotic activities has been established (13,14). The dual action of the different GAGs on huPK inactivation could be explained by analyzing their carbohydrate composition. It is known that GAGs contain iduronic acid and sulfate. Our results showed that chondroitin sulfate, which does not contain iduronic acid, has no significant action on huPK inhibition while bovine dermatan sulfate, that contains more iduronic acid than tuna dermatan sulfate, increases the inhibition. Heparin and heparan sulfate, having high sulfate content, also increase the inhibition.
The mechanism by which glycosaminoglycans interfere with huPK activity and inhibition is not understood. However, some possible mechanisms have been postulated by others (8,15): i) GAGs may bind to enzymes inducing a conformational change, leading to a more or less active form; ii) GAGs may bind to natural substrates or inhibitors, neutralizing their positive charges and interfering with their hydrolysis or inhibition.
It has been reported that some circulating proteins such as factor XI contain structural elements involved in the interactionswith GAGs (16). These elements include basic amino acids that form positively charged bindingsites for the negatively charged GAGs (17). Thrombin, however, comprises a more complex bindingregion involving charged residues that are not essentiallyadjacent to each other in the primary amino acid sequence, butare close together in the correctly folded protein (18,19). Since huPK shares high structural homology with factor XI (20), it was not surprising to observe that consensus sequences related to heparin binding sites are present in huPK.
The physiological role of the presumed interactions between huPK, GAGs and natural substrates and inhibitors is not known. However, since some GAGs such as dermatan sulfate have no deleterious effects on the organism, they appear to be candidates for therapeutic use in processes in which kallikrein participates such as fibrinolysis and blood coagulation or in processes involving uncontrolled huPK activity.
Address for correspondence: M.S. Araújo, Departamento de Bioquímica, EPM, UNIFESP, Rua Três de Maio, 100, 6º andar, 04044-020 São Paulo, SP, Brasil. Fax: +55-11-5572-3006. E-mail: mariana.bioq@unifesp.epm.br
Presented at SIMEC 2002 (International Symposium on Extracellular Matrix), Angra dos Reis, RJ, Brazil, October 7-10, 2002. Research supported by FAPESP, CNPq and FADA (UNIFESP-SPDM). Received November 29, 2002. Accepted May 29, 2003.
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Publication Dates
-
Publication in this collection
23 July 2003 -
Date of issue
Aug 2003
History
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Accepted
29 May 2003 -
Received
29 Nov 2002