Validation of transit-time flowmetry for chronic measurements of regional blood flow in resting and exercising rats

The objective of the present study was to validate the transit-time technique for long-term measurements of iliac and renal blood flow in rats. Flow measured with ultrasonic probes was confirmed ex vivo using excised arteries perfused at varying flow rates. An implanted 1mm probe reproduced with accuracy different patterns of flow relative to pressure in freely moving rats and accurately quantitated the resting iliac flow value (on average 10.43 ± 0.99 ml/min or 2.78 ± 0.3 ml min-1 100 g body weight -1). The measurements were stable over an experimental period of one week but were affected by probe size (resting flows were underestimated by 57% with a 2-mm probe when compared with a 1-mm probe) and by anesthesia (in the same rats, iliac flow was reduced by 50-60% when compared to the conscious state). Instantaneous changes of iliac and renal flow during exercise and recovery were accurately measured by the transit-time technique. Iliac flow increased instantaneously at the beginning of mild exercise (from 12.03 ± 1.06 to 25.55 ± 3.89 ml/min at 15 s) and showed a smaller increase when exercise intensity increased further, reaching a plateau of 38.43 ± 1.92 ml/min at the 4th min of moderate exercise intensity. In contrast, exercise-induced reduction of renal flow was smaller and slower, with 18% and 25% decreases at mild and moderate exercise intensities. Our data indicate that transit-time flowmetry is a reliable method for long-term and continuous measurements of regional blood flow at rest and can be used to quantitate the dynamic flow changes that characterize exercise and recovery. Correspondence


Introduction
There is increasing interest in the continuous measurement of total and regional blood flow in conscious animals.Electromagnetic (1-7) and pulsed Doppler ultrasonic flow probes (4,5,(8)(9)(10) have been widely used for these measurements but they present some disadvantages.Electromagnetic probes are large, not appropriate for small vessels and show a variable zero offset, limiting their use in long-term studies, while Doppler probes measure only flow changes and not the absolute values.In recent years, the development of transit-time probes has renewed the interest in these studies by providing a technique to continuously measure control values as well as changes in volumet-ric blood flow.Perivascular transit-time probes measure blood flow by emitting a plane of ultrasonic sound that transits the cross section of the vessel in both the upstream and downstream directions.The difference between the integrated transit-times of accelerated (directed downstream) and decelerated (directed upstream) ultrasonic signals, that interact with the volume flow occurring within the vessel, is a measure of volume per minute (ml/min or l/min).Since the transit-time technique gives absolute flow, independently of vessel size, and is easily implantable due to the reduced dimensions of the apparatus, it is adequate for chronic studies.In the rat, transit-time flowmetry has been widely used to quantitate blood flow in gastric (11,12), renal (13,14), mesenteric (13,15), carotid (13), and femoral (13) arteries and in the aorta (13,15) but only under acute conditions.
The validation of transit-time probes has been done in large animals under a variety of experimental conditions (3,7,16,17).In the rat, renal (14), mesenteric and aortic flows (15) were measured with Transonic probes and compared with known blood/saline flows obtained from pump-perfused circuits (15) or by clearance of p-aminohippuric acid (14).These data, obtained in acute experiments, have demonstrated the accuracy of the transit-time method for blood flow measurements.No information on the stability of chronic measurements by the transit-time probe is available for conscious freely moving rats.
All previous studies concerning regional blood flow changes induced by exercise in rats (18)(19)(20)(21) were conducted using the microsphere technique, which only provides information about static but not dynamic flow behavior.There is no information about continuous measurements and/or time-course changes of regional blood flow during maintained exercise and recovery.Studies of continuous blood flow changes simultaneous to pressure/heart rate changes during exercise are also lacking.In order to standardize the transit-time flowmetry for recording regional blood flow in rats, the specific objectives of the present study were 1) to validate true flow values obtained in vitro with 1-mm and 2-mm probes, 2) to determine the sensitivity of this technique in reproducing the instantaneous changes of flow simultaneously with pressure in freely moving rats, 3) to evaluate the accuracy of the 1-mm probe in recording sequentially the basal iliac flow (IF) after chronic implantation and 4) to describe the dynamic behavior of hindlimb and renal flow at rest, during exercise and recovery.

In vitro perivascular flow probe testing
The flow probes were submitted to bench tests before each implantation to confirm their performance.The values of the test signal were high (0.9 ± 0.06 to 1.02 ± 0.05 V) as recommended by the manufacturer and did not change throughout the experiment.
To assess the validity of 1-mm and 2-mm flow probe measurements over a wide range of flow values, a first series of experiments was conducted in vitro.The common carotid artery was removed from rats immediately after sacrifice under deep pentobarbital anesthesia and mounted in a circuit driven by a peristaltic (LKB, Bromma, 2115, multiperpex) or syringe pump (Orion, M 362).Transit-time ultrasonic probes (Transonic System, Ithaca, NY) were adapted around the vessel and an acoustic couplant was applied to fill the space between the carotid and transit-time probe and to provide adequate acoustic conduction.The circuit was filled with saline (transit-time flow measurements are independent of hematocrit) (14) and its extremity was opened into a graduated test tube to confirm the true flow.A wide range of known flow rates from 0 to 20-30 ml/min was passed through the artery and measured with the flowmeter (Transonic System, T 206 dual channel).

In vivo studies
All surgical procedures and protocols used were in accordance with the Institutional Guidelines for Animal Experimentation.
Male Wistar Kyoto rats (250-300 g, 2-3 months old) were anesthetized with sodium pentobarbital (50 mg/kg, ip).Two 1-cm dorsal incisions were made in the skin overlying the midscapular and iliac regions, and a blunt hemostat was used to create a subcutaneous tunnel between them.A midline incision was performed in the abdominal wall and the left iliac artery just below the aortic bifurcation (hindlimb studies) or the left renal artery (renal blood flow studies) was exposed and isolated for probe placement.All fatty tissue was carefully removed from the isolated segment of the vessel to avoid obstruction of the ultrasonic signal.The 1-mm (or 2-mm in one of the protocols) transit-time ultrasonic flow probe (model 1RB) was placed on the cleared section of the vessel.The probe was sutured to the underlying musculature and the segment of the artery containing the probe was covered with Lyostypt (Braun, Melsungen, Germany) to maintain vessel/probe alignment and to inhibit fat infiltration.The probe cable was passed through a small incision made in the retroabdominal wall and tunneled subcutaneously to the dorsal midscapular region.The probe was tested for the presence of a good acoustic signal, and blood flow during anesthesia was recorded for a period of 10-20 min (Gould recorder, model 4000, Cleveland, OH).The abdominal incision was then sutured and the connector end was fixed to the skin.The rats were treated with 60,000 units (0.1 ml, im) of penicillin (Pentabiótico Veterinário Fontoura, São Paulo, SP, Brazil).To record blood pressure, a clear vinyl catheter (3 cm of #10 tubing connected to 13 cm of #90 tubing) was inserted into the right iliac artery through the femoral artery of ether-anesthetized rats.The external part of the cannula was tunneled subcutaneously through the midscapular re-gion.The rats were allowed to recover for a period of 24 h.

Experimental protocols
Four groups of rats were studied.In group I, 10 conscious rats implanted with a 1-mm probe into the iliac artery were submitted to daily blood flow and heart rate (HR) measurements (40-60 min experimental sessions) for 5 days.The experimental sessions were conducted at the same time (about 8-10 a.m.).All behavioral activities (exploring, alert, rest, etc.) exhibited by the rats were annotated.Daily blood flow and heart rate values considered for each rat were those representing the respective means of all resting periods recorded.On the 5th day, the rats had the arterial cannula implanted and the recording session was repeated on the next day to assess the accuracy of the probe in detecting the possible patterns of iliac flow with respect to spontaneous pressure oscillations in conscious freely moving rats.The rats were then anesthetized with ether and the abdomen was reopened to expose the iliac artery plus the probe.The probe was calibrated for zero baseline function blood flow using the internal calibration system of the flowmeter.Under conditions of zero blood flow in the calibration meter, the normal ultrasound upstream/downstream flow sampling sequence of the flowmeter is replaced with unidirectional signal transmission passing through the probe.Thus, the flowmeter calculates blood volume flow from two identical measurements resulting in a zero flow output regardless of the actual blood flow in the vessel.The blood flow recording mode was turned on to reestablish the actual flow measurement, and the distal aorta was mechanically occluded just above the bifurcation leading to true zero flow in the vessel.This true zero blood flow reading was compared to the zero blood flow measurement of the flowmeter to ensure the validity of the zero calibration system.
Other groups of rats chronically implanted with a 1-mm probe in the iliac artery (group II, N = 7) or in the renal artery (group III, N = 13) were tested for regional blood flow changes during exercise.Before probe implantation the rats were adapted to 5-min treadmill running episodes of 0.3 to 0.8 km/ h, 0% degree for one week.The regional flow during rest, running and recovery was recorded on 3 successive days: on the first and second recording sessions, held 24 and 48 h after probe implantation, blood flow and heart rate were measured continuously.After stabilization of the variables, the rat ran on a treadmill (Inbramed, Porto Alegre, RS, Brazil) adapted for rats.The exercise test consisted of 4 min of graded exercise: 2 min at 0.4 km/h and 2 min at 0.8 km/h, 0% degree.On the second day the rats were implanted with the arterial cannula into the contralateral femoral artery and the running protocol was repeated 24 h later.To assess the reproducibility of IF, arterial pressure (AP) and HR changes during exercise, 2 sessions of running episodes were held for each rat, 40-60 min apart.Before the running protocol, IF, HR and AP were recorded for 15 to 30 min to permit stabilization of the variables and to obtain true resting values.
Group IV consisted of 8 rats with a 2-mm probe (model 2SB) chronically implanted around the iliac artery.IF records were repeated on 3 consecutive days (40-60 min experimental sessions) in freely moving rats.

Statistical analysis
Data are reported as mean ± SEM.The differences between groups were evaluated by the paired t-test or by analysis of variance for repeated measurements as appropriate.The level of significance was set at P<0.05.

Standardization of 1-mm and 2-mm transittime probes: in vitro validation and in vivo stability
Figure 1 provides the pooled data for relationships between true flow rate and measured flow with both probe sizes in the excised carotid artery.Under these experimental conditions the slopes for the 1-mm and 2-mm probes were quite different: 1.002 with a correlation coefficient of 0.997, and 0.778 with a correlation coefficient of 0.617 for probes 1R and 2S, respectively.Although data of carotid flow obtained with the 1-mm flow probe were superimposed to the line of identity, those obtained with the 2-mm probe were not.These results indicate that flow in the common carotid and iliac arteries is better quantitated by the 1-mm flow probe.
Iliac flow measurements after chronic implantation presented excellent stability.As observed in Figure 2 (upper panel for one conscious rat), both baseline HR and resting IF (pulsatile and mean) were stable and closely similar from day 2 up to day 6 after implantation.For this group, mean IF was on average 10.43 ± 0.99 ml/min, a value that was almost constant throughout the experimental period (Figure 2, lower panel).At rest, HR oscillated between 367 ± 15 and 398 ± 24 bpm during the 6 days of the experimental period.On the 6th day basal mean arterial pressure (MAP) was 110 ± 3 mmHg.It should be noted that at that time (after catheterization of the contralateral femoral artery) mean resting IF was 10.10 ± 0.7 ml/min.In contrast, the IF of conscious rats at rest differed markedly from the IF measured during surgery after acute implantation of the probe into pentobarbital-anesthetized rats.As illustrated in Figure 2, the IF of the same rats (4.46 ± 0.56 ml/min) was significantly reduced by 50-60% during anesthesia when compared to the conscious state.

Accuracy of probe measurements
The sensitivity of the transit-time probe in reproducing physiological changes of IF was analyzed in chronically implanted rats also instrumented with an arterial catheter.As shown in Figure 3, IF records reproduced not only tonic but also phasic instantaneous absolute changes of pulsatile and mean flow, i.e., all the physiological patterns of flow changes such as increases/decreases simultaneous to elevations/falls in pressure (panels A and B), no changes regardless of pressure changes (panel C) and phasic flow oscillations opposite to pressure oscillations (panel D) were observed.Maintenance of elevated flow (tonic effect) simultaneously to pressure oscillation was also observed (panel E).Most of these flow patterns were presented by the 10 freely moving rats of group I.The flow changes related or not to pressure modification seemed to depend on the behavioral activities and demonstrated that the probe was able to detect with accuracy all patterns of IF changes.

Zero flow baseline measurement
The accuracy of flow measurements was tested in situ after median laparotomy and exposition of the distal abdominal aorta and iliac artery implanted with the probe (ether anesthesia).Figure 4 shows a typical recording with the 1-mm probe taken from 2 rats.Brief occlusions (20 to 60 s, panels B and C, respectively) actually caused zero pressure and true zero flow in the iliac artery which were properly detected by the probe.Mean flow measured after abdominal aortic occlusion in the group of rats as a whole was 0.33 ± 1.3 ml/min, which was not different from zero.Also indicative of probe accuracy in detecting flow changes was the observation of the transient increase that characterizes the reactive hyperemic response after 1-min occlusion of the abdominal aorta (Figure 4,

Regional flow changes during exercise
The behavior of IF in response to dynamic exercise was tested simultaneously with AP records in another group of chronically instrumented rats during short 4-min runs on a treadmill.As illustrated in Figure 5, AP showed a small but similar increase (+10 to +15 mmHg) during the running period in rats implanted with iliac or renal probes.HR and IF increased quickly during the first 15-30 s of mild exercise (0.4 km/h), with an additional increase being observed at the beginning of moderate exercise intensity (0.8 km/ h), and remained at this level until the end of the exercise.In contrast, renal flow (RF) showed opposite and smaller changes, i.e., it was almost unchanged at the beginning of exercise and showed a slow and continuous decrease until the end of the exercise protocol.
Table 1 shows the baseline values of MAP, HR and regional blood flow obtained for the iliac and renal groups (protocols II and III).Baseline MAP, HR and absolute iliac and renal flow were similar for both groups.The renal group also had a smaller body weight (366 ± 17 vs 458 ± 18 g in the iliac group) with a similar relative flow (3.04 ± 0.5 vs 2.78 ± 0.3 ml min -1 100 g -1 ).On the other hand, calculations of flow index by correcting for kidney and hindlimb muscle weight (on average 1.37 g and 7.49 g, respectively) showed a higher value for renal tissue (7.93 vs 1.7, renal and hindlimb index, respectively).
The flow changes during exercise for both groups are illustrated in Figure 6.During mild exercise the first workload mean IF was increased by +15.1 ± 3.1 ml/min, a value that is more than double (114% increase) the resting IF, reaching +20.43 ± 2.45 ml/min (+158%) at the second workload.At the same exercise intensity, RF showed minor changes compared to baseline, i.e., RF decreased on average 2 ml/min (-18%) during the second minute of 0.4 km/h and 3-4 ml/ min at 0.8 km/h (-25% below the resting value).As observed in Figure 6, the recovery of both IF and RF was progressive, reaching basal values 3-4 min after the end of exercise.
IF changes during acute exercise were reproducible.In two successive runs on a treadmill 40-60 min apart, similar AP, HR and IF responses were observed (Table 2): for the same AP changes, IF was increased 2.1 and 2.6 times for 0.4 and 0.8 km/h in the first test and 2.1 and 2.5 times in the second test and a complete recovery of baseline values occurred 5 min after the end of exercise.

Absolute flow measurements: 1-mm vs 2-mm probes
An important and unexpected observation concerning absolute blood flow values is illustrated in Figure 7. Comparing IF values obtained with the 1-mm probe (group I) with the values presented by another group of rats chronically implanted with the 2-mm probe (group IV, N = 8), we observed that probes of different sizes showed different absolute IF values for the same condition, i.e., conscious state at rest.The values obtained with the large probe were significantly lower (57% on average) than those observed with the 1-mm probe.It should be noted that the average value for the 3-day period (4.45 ± 0.19 ml/min) was similar to that observed in vitro for a true flow of 10 ml/min (Figure 1).

Postmortem findings
No macroscopic evidence of iliac or renal artery damage was found in any group of rats instrumented with the transit-time flow probes.There was also no evidence of disturbance of hindlimb or renal blood flow  since the performance of rats on the treadmill after probe implantation was similar to that observed 1-2 days before surgery.

Discussion
In the present study we demonstrate for the first time that a transit-time probe permits sequential and accurate measurement of regional blood flow responses during rest, exercise and recovery.In addition, we showed that after chronic implantation the technique provided stable and reliable measurements, thus permitting continuous (and simultaneously to pressure) recording of tonic flow as well as its phasic changes.In contrast to previous reports that blood flow is independent of the size of the transit-time probe (14,15), we showed that 1-mm and 2-mm probes gave different absolute flow values, both in vitro and when chronically implanted, indicating that volumetric flow measurements are limited by the window of accuracy, which varies with probe size.
The transit-time technology for measuring dynamic changes of regional blood flow has several advantages over other methods such as electromagnetic, microsphere and Doppler system flowmetry: 1) small probe size permitting easy implantation; 2) continuous measurement of absolute blood flow representing an enormous advantage over phasic (microspheres, dye dilution) (3,22,23) or relative measurements (3,4,(8)(9)(10); 3) probes are nonconstrictive and therefore do not interfere with the pulse pressure or percentage of diastolic flow as electromagnetic devices do (7,14); 4) the probes are suitable for long-term studies (17) since the quality of the received signal improves with reactive infiltrating tissue growth around the probe/ vessel, providing the necessary acoustic conduction (3,15), and 5) the flowmeter zero offset function permits frequent calibration in conscious freely moving rats.The perivascular transit-time probe has been successfully used for continuous assessment of flow in coronary (17), mesenteric (13,(15)(16)(17), renal (13,14,17,24), carotid (13), gastric (11,12), hindlimb (13,17,25,26) and pulmonary arteries (7) and also for measurement of cardiac output (3,8,13,15,24).Most of these studies, however, were carried out after acute implantation and few were conducted after chronic implantation.Chronic implantation, however, was employed to study drug effects on cardiac output and regional flows (8) and did not attempt to describe the daily flow profile of resting animals (17,25).The results of the present experiments showed that implantable transit-time probes provided stable resting iliac flow values throughout the experimental period of 1 to 6 days.Another important result was the fact that the mean iliac flow of awake rats was not affected by arterial catheter placement in the contralateral femoral artery.These observations indicate that perivascular transit-time probes are suitable for chronic flow/pressure studies from the first day after implantation.In addition, the flow records obtained simultaneously to blood pressure records in awake rats demonstrated that transit-time probes reliably reproduce different flow patterns (mean and/or pulsatile) conditioned by pressure oscillations, opposite to pressure changes or bearing no relationship to them.Brief pulsatile records of femoral flow have already been reported for conscious rats (25), but they were obtained immediately before tail suspension and did not represent the true baseline flow.Bednarik and May (17) also employed this technique to measure mesenteric, renal, iliac and coronary blood flows  6).The present protocol also included comparisons of zero blood flow obtained by the zero flow baseline function of the flowmeter and by measurement of zero blood flow after complete occlusion of the vessel.The two procedures provided similar measurements and, since there were no detectable changes in zero baseline function during the experimental protocol, we confirmed the advantage of the ultrasonic method over electromagnetic flowmetry in chronic studies.These observations agree with other studies (13,15,17).An important and unexpected observation was the difference between the 1-mm and 2-mm probes concerning the resting iliac flow values of conscious rats.This discrepancy could be explained by the different windows of accuracy for the 1-mm and 2mm probes.D'Almeida et al. (15) reported that true flow for the 2-mm probe is identical to measured flow only for values of about 20 ml/min (ranging from 10.4 to 45 ml/min), with marked deviations occurring at smaller or larger flow values.Our data with the 2mm probe also showed deviations from true flow, but extending for all ranges of flow tested.On the other hand, our data with the 1-mm probe indicated a perfect correlation between measured and true flows, confirming previous observations (15) and strongly suggesting that the flows measured with the 1-mm probe accurately represent the true flow rates.Based on these observations, we suggest that the resting iliac flow of awake normotensive rats is about 2.8 ml min -1 100 g body weight -1 or 1.7 ml min -1 g tissue -1 .
We also showed that measurements in conscious and anesthetized rats yielded different values even when using the same probe.It is well known that pentobarbital anesthesia depresses respiratory function, decreases sympathetic vasoconstrictor tone and increases vasodilator tone, thus reducing stroke volume, central venous pressure and arterial pressure (27).Accordingly, during anesthesia the iliac flow was proportionally decreased when compared to the conscious state.In keeping with these observations, care should be taken when comparing flow profiles obtained with different transit-time probe sizes during different states (conscious vs anesthetized) and by different methods.Our absolute values are also in agreement with those obtained by Iriuchijima (1) who found a resting terminal aortic flow of 5.8 ml min -1 100 g -1 , a value similar to that obtained here for iliac flow if we consider that aortic flow is shared by each iliac artery.It should be noted that the flow index calculated from the present data confirmed the well-known concept of flow distribution, i.e., that the renal circulation receives a larger portion of cardiac output than the skeletal muscle circulation under resting conditions.
One important observation is that the perivascular probe is an excellent method for measuring the dynamic changes in regional blood flow during exercise and recovery.It permits detection of instantaneous and continuous changes of mean or pulsatile flow profile, reproducing the actual flow pattern of different territories such as iliac (marked increase) and renal arteries (small decrease) in response to mild to moderate exercise intensity.The very rapid and pronounced increase of iliac flow was determined by withdrawal of sympathetic tone at the beginning of exercise, associated with local metabolic factors whose accumulation was proportional to the exercise intensity (28).On the other hand, the small reduction of renal flow during exercise was not unex-pected due to autoregulatory responses (29).Whether renal flow is maintained or decreased during exercise remains controversial (30).Most evidence suggests that redistribution of renal flow to exercising muscles only occurs when the exercise intensity is sufficiently severe and/or when a decrease in oxygen delivery to working muscles is imposed (30).Dynamic regional flow patterns during exercise have not been studied previously mainly due to the lack of an appropriate technique.Blood flow distribution within and among muscles in rats (18)(19)(20)(21) and dogs (30) during exercise was determined by means of radiolabeled and radioactive microsphere techniques which permit few and static flow measurements, but do not provide information about the dynamic moment-to-moment flow changes as obtained here with the perivascular ultrasonic probe.
Based on the present observations, we propose that the transit-time technique is adequate to reproduce with accuracy any flow profile independent of absolute change (increase/decrease) and time-course (instantaneous or phasic/maintained or tonic).
In summary, the transit-time flow probe provides important advantages over other systems for the measurement of regional blood flow.It provides direct flow volume measurements, has a negligible zero offset and presents a long in vivo life span, thus permitting accurate measurements of regional flow after chronic implantation over long periods.It is a good and reliable method to measure instantaneously and continuously the dynamic changes of blood flow during rest, exercise and recovery in small animals such as rats.

Figure 1 -
Figure 1 -In vitro calibration of 1-mm (upper panel) and 2-mm (lower panel) probes implanted around the rat common carotid artery.Symbols represent experimental data obtained with 1mm (N = 4) and 2-mm (N = 2) probes.The solid line represents the slope of the data and the dotted line, the line of identity.Parameters of linear regression analysis are shown.

Figure 2 -
Figure 2 -Upper panel, Sequential recordings of heart rate (HR) and pulsatile and mean iliac flow (IF) at rest in a conscious rat with a perivascular 1-mm probe chronically implanted around the iliac artery.Lower panel, Mean values of resting iliac flow obtained on 6 consecutive days in the group of 10 rats.The bar represents the flow of the same rats immediately after probe implantation and during pentobarbital anesthesia.*P<0.05 compared to the conscious state (paired t-test).

Figure 3 -Figure 4 -
Figure 3 -Records of pulsatile (AP) and mean arterial pressure (MAP), heart rate (HR) and mean or pulsatile iliac flow (IF) in freely moving rats with a 1-mm probe chronically implanted around the iliac artery.See text for explanation.
panel C).Moreover, the flowmeter zero flow function (Figure4, panel A) produced zero flow recordings similar to true zero flow measurements (panels B and C).Since no differences between the two methods for measuring zero blood flow were observed, the zero baseline function was used for calibration during the chronic experiments.We zero flow in some animals by measuring IF in situ after sacrifice at the end of the experiments.

Figure 5 -
Figure 5 -Recordings of pulsatile (AP) and mean arterial pressure (MAP), heart rate (HR) and mean regional flow (BF) for 2 rats with a chronically implanted 1-mm probe (left panel, iliac flow and right panel, renal flow) during rest, exercise of mild (0.4 km/h) to moderate (0.8 km/h) intensity (black bars) and recovery.

Figure 6 -
Figure 6 -Time-course changes of absolute regional flow (BF) (iliac, N = 7 and renal, N = 10) during 4 min of exercise (black bar) and during recovery.

Figure 7 -
Figure 7 -Comparison of absolute iliac flow values (IF) obtained in the conscious state at rest (day 1 up to day 3) for rats implanted with a 1-mm (group I, N = 10) or 2-mm probe (group IV, N = 8).*P<0.05 compared to the 1-mm probe (ANOVA).

Table 2 -
Comparison of mean arterial pressure (MAP), heart rate (HR) and iliac flow (IF) values in 2 running tests involving 7 normotensive rats.