[RETRACTED ARTICLE] Therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis

Wang Hai Xu Ping Yang Zhi-wen Zhang Chun About the authors

Abstract

Caspase recruitment domain-containing protein 9 (Card9) is located upstream of the nuclear factor kappa B (NF-κB) and p38 mitogen-activated protein kinase (MAPK) inflammatory pathways. This study investigated the therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis (SAP). SAP was induced by a retrograde infusion of 5.0% sodium taurocholate into the biliopancreatic duct of Sprague Dawley rats (n=54), which were then treated with pioglitazone. Blood and pancreatic tissues were harvested at 3, 6, and 12 h after SAP induction. Pancreatic pathological damage was evaluated by hematoxylin and eosin staining. Serum amylase, serum pro-inflammatory cytokines, and pancreatic myeloperoxidase (MPO) activities were determined by enzyme-linked immunosorbent assay. The expression of Card9 mRNA and protein in pancreatic tissues was detected by real-time polymerase chain reaction and western blotting. Pioglitazone had a therapeutic effect in treating rats with SAP by decreasing the level of amylase activity, ameliorating pancreatic histological damage, decreasing serum pro-inflammatory cytokine levels and tissue MPO activity, and downregulating the expression of NF-κB, p38MAPK, and Card9 mRNAs and proteins (P<0.05). The present study demonstrated that the inhibition of Card9 expression could reduce the severity of SAP. Card9 has a role in the pathogenic mechanism of SAP.

Caspase recruitment domain-containing protein 9; Pioglitazone; Severe acute pancreatitis


Retraction for: Braz J Med Biol Res | doi: 10.1590/1414-431X20176812 | PMID: 5731332

The authors would like to retract the article “Therapeutic effect and potential mechanism of pioglitazone in rats with severe acute pancreatitis” that was published in volume 51 no. 2 (2018) (Epub Dec 11, 2017) in the Brazilian Journal of Medical and Biological Research <http://dx.doi.org/10.1590/1414-431x20176812> PMID: 5731332.

The first author Wang Hai states that “we want to do further study about the part of validation of NF-κB, p38MAPK proteins”.

The Brazilian Journal of Medical and Biological Research had received authorization from all authors before the publication of the paper. We regret the unprofessional behavior of the authors involved.

Publication Dates

  • Publication in this collection
    2018

History

  • Received
    26 June 2017
  • Accepted
    15 Sept 2017
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