Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx Please cite this article as: Ribeiro CV, Vasconcelos AC, Andrade Filho JS. Apoptosis and expression of argyrophilic nucleolus organizer regions in epithelial neoplasms of the larynx. Braz J Otorhinolaryngol. 2015;81:158-66. ☆☆ ☆☆ Institution: This project was developed at the Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG); and Escola de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. ☆☆☆ ☆☆☆ Case report: a retrospective study of 40 cases of epithelial neoplasms of the larynx, with 3 degrees of tumor differentiation.

Christiana Vargas Ribeiro Anilton Cesar Vasconcelos José de Souza Andrade FilhoAbout the authors

INTRODUCTION:

Occurrence of apoptosis and expression of proliferative markers are powerful tools to establish a prognosis in the follow-up of cancer.

OBJECTIVE:

To evaluate the growth fraction in papillomas and laryngeal squamous cell carcinomas with three degrees of differentiation through apoptosis and the expression of nucleolus organizer regions.

METHODS:

Retrospective study from which paraffin material was submitted to microtomy and hematoxylin-eosin and silver staining. Stained slides were used to quantify the apoptotic index and the number of nucleolus organizer regions by morphometry.

RESULTS:

Apoptosis was significantly more frequent in well differentiated carcinomas and in papillomas, and a higher growth fraction of expressed nucleolus organizer regions and cells that expressed a greater than average number of nucleolus organizer regions were more frequently noted in undifferentiated carcinomas.

CONCLUSIONS:

Thus, it was possible to verify that a high apoptotic index was associated with a lower chance of tumor differentiation in carcinomas, while a greater number of total nucleolus organizer regions, cells expressing nucleolus organizer regions above average and a higher growth fraction were associated with greater likelihood of abnormal cell proliferation and increased tumor differentiation.

Apoptosis; Larynx; Squamous cell carcinoma; Nucleolus organizer region; Papilloma


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