ABSTRACT
INTRODUCTION:
Several approaches have been tried for the treatment of tinnitus, from cognitive-behavioral therapies and sound enrichment to medication. In this context, antioxidants, widely used in numerous areas of medicine, appear to represent a promising approach for the control of this symptom, which often is poorly controlled.
OBJECTIVE:
To evaluate the effects of antioxidant therapy for tinnitus in a group of elderly patients.
METHODS:
Prospective, randomized, double-blinded, placebo-controlled clinical trial. The sample consisted of 58 subjects aged 60 years or older, with a complaint of tinnitus associated with sensorineural hearing loss. These individuals completed the Tinnitus Handicap Inventory (THI) questionnaire before and after six months of therapy. The treatment regimens were: Ginkgo biloba dry extract (120 mg/day), a-lipoic acid (60 mg/day) + vitamin C (600 mg/day), papaverine hydrochloride (100 mg/day) + vitamin E (400 mg/day), and placebo.
RESULTS:
There was no statistically significant difference between THI by degree (p = 0.441) and by score (p = 0.848) before and after treatment.
CONCLUSION:
There was no benefit from the use of antioxidant agents for tinnitus in this sample.
Keywords:
Tinnitus; Antioxidants; Aged
Resumo
Introdução:
Uma série de abordagens terapêuticas tem sido empregada no tratamento do zumbido, desde terapias cognitivo-comportamentais e de enriquecimento sonoro até terapias medicamentosas. Nesse contexto, os agentes antioxidantes, amplamente utilizados em diversas áreas da medicina, parecem representar uma perspectiva promissora para o controle desse sintoma, que muitas vezes tem um controle clínico insatisfatório.
Objetivo:
Avaliar os efeitos da terapia com agentes antioxidantes sobre o zumbido em um grupo de pacientes idosos.
Método:
Ensaio clínico prospectivo, randomizado, duplo-cego e controlado por placebo. A amostra composta de 58 indivíduos com 60 anos ou mais, com queixa clínica de zumbido associado à perda auditiva, do tipo neurossensorial, em graus variados. Esses indivíduos foram submetidos ao questionário THI (Tinnitus Handicap Inventory) antes e após 6 meses de uso da medicação. Os esquemas terapêuticos foram os seguintes: extrato seco de Ginkgo biloba(120 mg/dia), ácido a-lipóico (60 mg/dia) + vitamina C (600 mg/dia), cloridrato de papaverina(100 mg/dia) + vitamina E (400 mg/dia) e placebo.
Resultados:
O THI após o tratamento foi estatisticamente igual ao THI antes do tratamento, tanto em graus (p = 0,441) quanto em escores (p = 0,848).
Conclusão:
Não se verificou benefício estatisticamente significativo com o uso de agentes antioxidantes para o zumbido dos indivíduos avaliados.
PALAVRAS-CHAVE:
ZUMBIDO;ANTIOXIDANTES;IDOSO
Introduction
Tinnitus, whose prevalence is estimated at approximately 10% of the adult population, has repercussions and an impact on quality of life of the affected individuals, that varies from a slight perception without discomfort to an extreme compromise in quality of life.11. Davis A, El Rafaie A. Epidemiology of tinnitus. In: Tyler RS, editor. Tinnitus handbook. San Diego: Thomson Learning; 2000. p. 1-23.
For those whose tinnitus has significant clinical impact, a number of therapeutic approaches have been described and employed, from cognitive-behavioral therapies and sound enrichment, to drug approaches. Some studies have shown favorable results, while others did not result in benefits.22. Baguley D, McFerran D, Hall D. Tinnitus. Lancet. 2013;382: 1600-7. Various substances have been used and tested as drug treatments. Among them, antioxidants have appeared promising.22. Baguley D, McFerran D, Hall D. Tinnitus. Lancet. 2013;382: 1600-7. Antioxidants encompass a wide range of substances whose primary function is the neutralization and clearance of free radicals, that is, because of their molecular configuration, result in being toxic and harmful to cells and tissues. With respect to the auditory system, the action of free radicals in cochlear physiology has been demonstrated experimentally.3, 4, 5 and 6 In the case of auditory disorders, antioxidants have been used in sudden deafness, to try to prevent ototoxicity, and for acute acoustic trauma,77. Joachims HZ, Segal J, Golz A, Netzer A, Goldenberg D. Antioxidants in treatment of idiopathic sudden hearing loss. Otol Neurotol. 2003;24:572-5.and88. Haase GM, Prasad KN, Cole WC, Baggett-Strehlau JM, Wyatt SE. Antioxidant micronutrient impact on hearing disorders: concept, rationale, and evidence. Am J Otolaryngol. 2011;32: 55-61. as well as in the approach to presbycusis, sometimes with conflicting results.99. Takumida M, Anniko M. Radical scavengers for elderly patients with age-related hearing loss. Acta Otolaryngol. 2009;129:36-44.and1010. Polanski JF, Cruz OL. Evaluation of antioxidant treat-ment in presbycusis: prospective, placebo-controlled,double-blind, randomised trial. J Laryngol Otol. 2013;127:134-41. In cases of tinnitus, probably the substance most widely used and studied currently is Ginkgo biloba, an herbal antioxidant. Associations of antioxidants, vitamins, and phospholipids administered to patients diagnosed with idiopathic tinnitus demonstrated relief of this condition and decreased serum levels of free radicals in a case series study. 1111. Savastano M, Brescia G, Marioni G. Antioxidant therapy in idiopathic tinnitus: preliminary outcomes. Arch Med Res. 2007;38:456-9.
Thus, it was decided to test the effects of antioxidants on tinnitus in a group of elderly patients in a controlled clinical study.
Methods
The research project was submitted to the Ethics Committee on Institutional Research and approved under No. CEP 0723/10.
The research was registered with the International Clinical Trials platform of the World Health Organization at: http://apps.who.int/trialsearch/trial.aspx?trialid=ACTRN12610000667011.
The sample was composed of 58 male and female subjects aged 60 years or older with clinical complaints of tinnitus associated with a variable degree of sensorineural hearing loss confirmed by previous audiometric testing. These subjects were administered the Tinnitus Handicap Inventory (THI) questionnaire1212. Newman CW, Jacobson GP, Spitzer JB. Development of the tinnitus handicap inventory. Arch Otolaryngol Head Neck Surg. 1996;122:143-8. before and after medication use. THI is a scale that measures discomfort caused by tinnitus, with questions related to everyday annoyances and losses attributed to the symptom, defining a different numeric value for each affirmative or negative answer, or for partial agreement. The final sum (score) is framed by a gradation (degree), from 1 (slight, only perceived in quiet environments) to 5 (catastrophic). In the sample selection, subjects with known allergy to any substance to be tested or with clinical contraindications to the use of these substances were excluded. Anticoagulant users or subjects with coagulopathy, as well as diabetics, were also excluded from the sample.
The entire group was interviewed in detail about their medical history, and the data were recorded. Then, participants were asked to give information about hearing loss duration, use (or not) of hearing aids, hypertension, dyslipidemia, heart disease, thyroid disease, and osteoarthropathy, and also in men, benign prostatic hyperplasia. The subjects were also asked about possible exposure to ototoxic substances or noisy environments, i.e., an exogenous auditory risk. In addition, a clinical exam was performed, focused on otoscopy.
Patients were treated for a period of six months. They were allocated into four groups and treated with one of the following regimens: dry extract of G. biloba (120 mg/day), a-lipoic acid (60 mg/day) plus vitamin C (600 mg/day), papaverine hydrochloride (100 mg/day) plus vitamin E (400 mg/day), and placebo (starch capsules). The substances were not identified by name in the containers into which they were packed, but rather through symbols defined by a professional who did not participate in the research, as a way of blinding investigators and patients. For distribution and randomization of participants, the resources available at http://www.randomization.com were used.
The statistical tests used in the analysis included Pearson's chi-squared test, Fisher's exact test (or its extension), and analysis of variance with parametric and non-parametric repeated measures. In all conclusions reached through the inferential analysis, the significance level a = 5% was used.
Results
The general epidemiological data of the sample are listed in Table 1.
The most significant general clinical data of the sample are listed in Table 2.
Distribution of the presence of comorbidities reported by patients with complaints of tinnitus.
Table 3 lists the distribution of THI by degree for subjects with complaints of tinnitus, in the different groups and in the time points before and after treatment.
Distribution of Tinnitus Handicap Inventory (THI) by degree of subjects with complaint of tinnitus, for placebo (P), Ginkgo biloba 120 mg/day (GB), a-lipoic acid 60 mg/day plus vitamin C 600 mg/day (AA + VC), and papaverine hydrochloride 100 mg/day plus vitamin E 400 mg/day (PP + VE) groups, before and after treatment time points.
Table 4 lists the distribution of THI in scores for subjects with complaints of tinnitus, in the different groups and in the time points before and after treatment.
Distribution of Tinnitus Handicap Inventory (THI) by score of subjects with complaint of tinnitus, for placebo (P), Ginkgo biloba 120 mg/day (GB), a-lipoic acid 60 mg/day plus vitamin C 600 mg/day (AA + VC), and papaverine hydrochloride 100 mg/day plus vitamin E 400 mg/day (PP + VE) groups, before and after treatment time points.
After statistical analysis, it was concluded that THI before treatment was statistically equivalent to THI after treatment, both by degree (p = 0.441) and by score (p = 0.848). Additionally, the inferential results revealed that the four treatment groups were statistically equivalent, both in THI expressed by degree (p = 0.663) and by score (p = 0.715).
Discussion
A number of antioxidants have been studied, showing positive effects in several clinical conditions.13, 14 and 15 In this study, the choice of the selected substances was based on the evidence and descriptions in the literature, both in clinical and experimental research, and also on their availability in this community. Briefly, G. biloba can be described as an herbal medicine whose active pharmacological groups are flavonoids with antioxidant and vasodilator action, and terpene lactones, which act as antiplatelet agents. 1616. Smith JV, Luo Y. Studies on molecular mechanisms of Ginkgo biloba extract. Appl Microbiol Biotechnol. 2004;64: 465-72. Originally, a-lipoic acid was considered as part of the vitamin B complex, but now is no longer considered as a vitamin, because there is evidence that this substance can be synthesized by the human body. a-Lipoic acid has an antioxidant effect and also an oxidative reduction effect on other antioxidants.1414. Bilska A, Wlodek L. Lipoic acid - the drug of the future? Pharmacol Rep. 2005;57:570-7. Vitamin E is an essential fat-soluble vitamin whose main function is related to the lipid stability of cell membranes against oxygen free radicals. This vitamin also has a modulating effect on cell growth, in response to oxidative stress, hence its positive effect on atherosclerosis and certain neoplasms.1717. Meydani M. Vitamin E. Lancet. 1995;345:170-5. Vitamin C or ascorbic acid is a water-soluble vitamin, critical for collagen and L-carnitine biosynthesis, for the conversion of dopamine to norepinephrine; it also improves iron absorption. Under physiological conditions, this vitamin also acts as a potent antioxidant.1818. Li Y, Schellhorn HE. New developments and novel therapeutic perspectives for vitamin C. J Nutr. 2007;137:171-84. Papaverine hydrochloride is a synthetic alkaloid that exerts a tissue protective effect correlated to antioxidants, because this substance promotes non-specific smooth muscle relaxation, leading to vasodilation.1919. Mathis JM, Jensen ME, Dion JE. Technical considerations on intra-arterial papaverine hydrochloride for cerebral vasospasm. Neuroradiology. 1997;39:90-8. Antioxidants act synergistically with other agents or in isolation, functioning in different ways, protecting cell membranes and also eliminating oxygen free radicals. 44. Seidman MD. Effects of dietary restriction and antioxidants on presbycusis. Laryngoscope. 2000;110:727-38.and66. Heman-Ackah SE, Juhn SK, Huang TC, Wiedmann TS. A combination antioxidant therapy prevents age-related hearing loss in C57BL/6 mice. Otolaryngol Head Neck Surg. 2010;143:429-34.
The afflictions of the auditory apparatus are complex conditions that involve a number of physical phenomena, various tissues, and different topographies of the auditory pathway. Tinnitus appears to be caused by abnormal neural activity in cochlea-auditory cortex pathway.2020. Eggermont J. Pathophysiology of tinnitus. Prog Brain Res. 2007;166:19-35. There is a consensus in the literature that at least some of the changes found along the auditory pathway and related to auditory symptoms appear to be related to biochemical changes, inflammation, and injuries induced by free radicals.88. Haase GM, Prasad KN, Cole WC, Baggett-Strehlau JM, Wyatt SE. Antioxidant micronutrient impact on hearing disorders: concept, rationale, and evidence. Am J Otolaryngol. 2011;32: 55-61.
The main cause of tinnitus is damage to hearing sensory cells of the cochlea, with or without association to an injury of central auditory system structures, through several etiopathogenic mechanisms.2121. Nondahl DM, Cruickshanks KJ, Huang GH, Klein BE, Klein R, Nieto FJ, et al. Tinnitus and its risk factors in the Beaver Dam offspring study. Int J Audiol. 2011;50:313-20. Subjects with normal hearing may also have tinnitus; however, patients with hearing loss may not have tinnitus. The sample of this study was entirely composed of subjects with tinnitus and sensorineural hearing loss.
With regard to the sample, anticoagulant users were excluded due to the chance of bleeding when these drugs are combined with G. biloba, which have an antithrombotic effect. Another group excluded was diabetics, due to the chance of glycemic imbalance when in combination with a-lipoic acid. 1414. Bilska A, Wlodek L. Lipoic acid - the drug of the future? Pharmacol Rep. 2005;57:570-7. Given the authors' intention to test several substances in a very defined population group, the groups had a relatively limited number of subjects, but they were methodologically sound for this study and its statistical evaluation.
The substances chosen were processed in a compounding pharmacy, since the combinations used are not commercially available. This was also important in the blinding process, since the substances were packaged in identical capsules and identical bottles, but were identified by different symbols, so they could not be identified by the subjects or researchers. The person responsible for handling the substances was aware of this condition. The doses of substances used were based on what the literature recommends as an effective and clinically safe dose. In this study, there were no adverse effects with these substances during the study period and at the doses used. Moreover, according to the literature and pharmacological research, there are no reports on the associations of substances proposed in this study, nor information to indicate that, once associated, they could show decreased interaction or summation of their effects. This was corroborated by our results, since no modification of the researched symptom occurred after treatment.
The effects of antioxidant therapy for tinnitus were evaluated through THI,1212. Newman CW, Jacobson GP, Spitzer JB. Development of the tinnitus handicap inventory. Arch Otolaryngol Head Neck Surg. 1996;122:143-8. a validated and widely used questionnaire to evaluate the influence of tinnitus on quality of life of the subjects tested. As described earlier, there was no observable effect of the antioxidants on tinnitus in the sample groups for a period of six months. Some reports indicate that the use of B-complex vitamins could be beneficial in controlling tinnitus. However, no controlled clinical trial has proven this hypothesis.2222. Seidman MD, Standring RT, Dornhoffer JL. Tinnitus: current understanding and contemporary management. Curr Opin Otolaryngol Head Neck Surg. 2010;18:363-8. Regarding G. biloba in the treatment of tinnitus, a systematic review evaluating studies on the use of this substance (in its EGb 761 presentation) has demonstrated efficacy when compared to placebo. 2323. Von Boetticher A. Ginkgo biloba extract in the treatmentof tinnitus: a systematic review. Neuropsychiatr Dis Treat.2011;7:441-7. Another Cochrane group review conducted in 2013 did not demonstrate efficacy of G. biloba in the treatment of tinnitus, irrespective of the form in which the plant extract was obtained. 2424. Hilton MP, Zimmermann EF, Hunt WT. Ginkgo biloba for tinnitus. Cochrane Database Syst Rev. 2013;3:CD003852. The present study used compounded dry extract of G. biloba.
These findings also corroborate a recent international recommendation, against prescribing vitamin and dietary supplements for the treatment of patients with persistent and clinically relevant tinnitus.2525. Tunkel DE, Bauer CA, Sun GH, Rosenfeld RM, Chandrasekhar SS, Cunningham ER, et al. Clinical practice guideline: tinnitus. Otolaryngol Head Neck Surg. 2014;151:1-40.
Conclusion
In the time interval and sample evaluated, we observed no statistically significant benefit from the use of antioxidants for tinnitus.
References
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1Davis A, El Rafaie A. Epidemiology of tinnitus. In: Tyler RS, editor. Tinnitus handbook. San Diego: Thomson Learning; 2000. p. 1-23.
-
2Baguley D, McFerran D, Hall D. Tinnitus. Lancet. 2013;382: 1600-7.
-
3Clerici WJ, Yang L. Direct effects of intraperilymphatic reactive oxygen species generation on cochlear function. Hear Res. 1996;101:14-22.
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4Seidman MD. Effects of dietary restriction and antioxidants on presbycusis. Laryngoscope. 2000;110:727-38.
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5Jacono AA, Hu B, Kopke RD, Henderson D, Van De Water TR, Steinman HM. Changes in cochlear antioxidant enzyme activity after sound conditioning and noise exposure in the chinchilla. Hear Res. 1998;117:31-8.
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6Heman-Ackah SE, Juhn SK, Huang TC, Wiedmann TS. A combination antioxidant therapy prevents age-related hearing loss in C57BL/6 mice. Otolaryngol Head Neck Surg. 2010;143:429-34.
-
7Joachims HZ, Segal J, Golz A, Netzer A, Goldenberg D. Antioxidants in treatment of idiopathic sudden hearing loss. Otol Neurotol. 2003;24:572-5.
-
8Haase GM, Prasad KN, Cole WC, Baggett-Strehlau JM, Wyatt SE. Antioxidant micronutrient impact on hearing disorders: concept, rationale, and evidence. Am J Otolaryngol. 2011;32: 55-61.
-
9Takumida M, Anniko M. Radical scavengers for elderly patients with age-related hearing loss. Acta Otolaryngol. 2009;129:36-44.
-
10Polanski JF, Cruz OL. Evaluation of antioxidant treat-ment in presbycusis: prospective, placebo-controlled,double-blind, randomised trial. J Laryngol Otol. 2013;127:134-41.
-
11Savastano M, Brescia G, Marioni G. Antioxidant therapy in idiopathic tinnitus: preliminary outcomes. Arch Med Res. 2007;38:456-9.
-
12Newman CW, Jacobson GP, Spitzer JB. Development of the tinnitus handicap inventory. Arch Otolaryngol Head Neck Surg. 1996;122:143-8.
-
13Byers T, Bowman B. Vitamin E supplements and coronary heart disease. Nutr Rev. 1993;51:333-6.
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14Bilska A, Wlodek L. Lipoic acid - the drug of the future? Pharmacol Rep. 2005;57:570-7.
-
15Mahadevan S, Park Y. Multifaceted therapeutic benefits of Ginkgo biloba L.: chemistry, efficacy, safety, and uses. J Food Sci. 2008;73:14-9.
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16Smith JV, Luo Y. Studies on molecular mechanisms of Ginkgo biloba extract. Appl Microbiol Biotechnol. 2004;64: 465-72.
-
17Meydani M. Vitamin E. Lancet. 1995;345:170-5.
-
18Li Y, Schellhorn HE. New developments and novel therapeutic perspectives for vitamin C. J Nutr. 2007;137:171-84.
-
19Mathis JM, Jensen ME, Dion JE. Technical considerations on intra-arterial papaverine hydrochloride for cerebral vasospasm. Neuroradiology. 1997;39:90-8.
-
20Eggermont J. Pathophysiology of tinnitus. Prog Brain Res. 2007;166:19-35.
-
21Nondahl DM, Cruickshanks KJ, Huang GH, Klein BE, Klein R, Nieto FJ, et al. Tinnitus and its risk factors in the Beaver Dam offspring study. Int J Audiol. 2011;50:313-20.
-
22Seidman MD, Standring RT, Dornhoffer JL. Tinnitus: current understanding and contemporary management. Curr Opin Otolaryngol Head Neck Surg. 2010;18:363-8.
-
23Von Boetticher A. Ginkgo biloba extract in the treatmentof tinnitus: a systematic review. Neuropsychiatr Dis Treat.2011;7:441-7.
-
24Hilton MP, Zimmermann EF, Hunt WT. Ginkgo biloba for tinnitus. Cochrane Database Syst Rev. 2013;3:CD003852.
-
25Tunkel DE, Bauer CA, Sun GH, Rosenfeld RM, Chandrasekhar SS, Cunningham ER, et al. Clinical practice guideline: tinnitus. Otolaryngol Head Neck Surg. 2014;151:1-40.
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☆
Please cite this article as: Polanski JF, Soares AD, de Mendonça Cruz OL. Antioxidant therapy in the elderly with tinnitus. Braz J Otorhinolaryngol. 2016;82:269-74.
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☆☆
Institution: Department of Otorhinolaryngology and Head and Neck Surgery, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Publication Dates
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Publication in this collection
May-Jun 2016
History
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Received
16 Dec 2014 -
Accepted
27 Apr 2015