Changes in activities of antioxidant enzymes in radish (<i>Raphanus sativus</i>) seedlings in response to allelopathic effect of safflower (<i>Carthamus tinctorius</i>)

Motamedi, Marzieh; Karimmojeni, Hassan; Sini, Fatemeh Ghorbani; Majidi, Mohammad Mahdi

doi:10.1590/s2175-97902022e19017

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Article • Braz. J. Pharm. Sci. 58 • 2022 • https://doi.org/10.1590/s2175-97902022e19017 linkcopy

Changes in activities of antioxidant enzymes in radish (Raphanus sativus) seedlings in response to allelopathic effect of safflower (Carthamus tinctorius)

Authorship SCIMAGO INSTITUTIONS RANKINGS

ROS (Reactive Oxygen Species) production is a usual plant reaction to environmental stresses such as allelopathy. Plants possess antioxidant enzymes to scavenge cells and resist against the ROS. This study was conducted to evaluate changes in antioxidant enzymes (CAT, GPX, APX) in radish seedlings in response to allelopathic effect of safflower root and shoot residues grown under normal irrigation and drought stress. Safflower allelopathic effect led to an increase in antioxidant enzymes activities. GPX activity increased more than CAT and APX. Radish seedlings exposed to safflower residue grown under drought stress showed more antioxidant enzymes activities. Root residues enhanced the activities of antioxidant enzymes greater than shoot. Seedlings exposed to root residues grown under drought stress had the highest level of antioxidant enzymes activities.

Key Words:
ROS; CAT; GPX; APX; Allelopathy

Source of variation	Degree of freedom	CAT	GPX	APX
Safflower variety	3	0.1697^**	29.6152^**	0.1043^**
Irrigation Level	1	0.0816^**	5.7228^**	0.0857^**
Safflower organ type	1	0.1699^**	16.0006^**	0.243^**
Safflower variety × Irrigation Level	3	0.0002 ^ns	0.0589 ^ns	0.0027 ^ns
Safflower variety ×	3	0.0002 ^ns	0.0589 ^ns	0.0027 ^ns
Safflower organ type	3	0.0025 ^ns	0.1166 ^ns	0.0049 ^ns
Safflower organ type × Irrigation Level	1	0.0015 ^ns	0.0382 ^ns	0.0065 ^ns
Safflower organ type ×	3	0.0024 ^ns	0.0294 ^ns	0.0003 ^ns
Irrigation Level ×
Safflower variety
Experimental error	32	0.0016	0.2050	0.0019
CV%		11.4242	12.4289	14.7209

Genotype type	CAT (unit mg^-1 pro min^-1)		GPX (unit mg^-1 pro min^-1)		APX (unit mg^-1 pro min^-1)
	Normal	Drought	Normal	Drought	Normal	Drought
Khorasan (Khorasan330)	0.5874 ^a	0.6891 ^a	4.1381 ^a	4.8998 ^a	0.3894 ^a	0.5260 ^a
Egypt (PI 657800)	0.4955^b	0.5533^b	3.9734 ^a	4.9156^a	0.3801^ab	0.4923^a
Kerman (CTNIR9)	0.3246 ^c	0.4324 ^c	1.1755 ^b	1.7807 ^b	0.2537 ^c	0.3482 ^b
Australia (PI 262424)	0.3797 ^c	^c 0.488	1.4814 ^b	2.1605 ^b	0.3013 ^b	0.3897 ^b
Control (Absence of residue)	0.2287^d	0.2287^d	1.1056^c	1.1056^c	0.2795^c	0.2795^c
LSD	0.0646	0.0625	0.7965	0.5975	0.1005	0.0793

Genotype type	CAT (unit mg^-1 pro min^-1)		GPX (unit mg^-1 pro min^-1)		APX (unit mg^-1 pro min^-1)
	Normal	Drought	Normal	Drought	Normal	Drought
Khorasan (Khorasan330)	0.7056 ^a	0.7889 ^a	5.338 ^a	5.8446 ^a	0.5999 ^a	0.7094 ^a
Egypt (PI 657800)	0.6293 ^a	0.7429 ^a	4.9431 ^a	5.944 ^a	0.5444^ab	0.6193^b
Kerman (CTNIR9)	0.4508 ^b	0.498 ^b	2.5993 ^b	3.0322 ^b	0.4509^bc	0.4121 ^d
Australia (PI 262424)	0.5231 ^b	0.5634 ^b	2.7328 ^b	3.3279 ^b	0.392 ^c	0.4904 ^c
Control (Absence of residue)	0.2287^c	0.2287^c	1.1056^c	1.1056^c	0.2795^d	0.2795^e
LSD	0.0995	0.0771	0.8273	1.169	0.0936	0.0524

	CAT Concentration	GPX Concentration	APX Concentration	Shoot lenght	Wet Weight
CAT Concentration	1.00000
GPX Concentration	0.92180^**	1.00000
APX Concentration	0.83181^**	0.84716^**	1.00000
Shoot lenght	-0.82953^**	-0.93620^**	-0.81008^**	1.00000
Wet Weight	-0.84956^**	-0.94896^**	-0.81884^**	0.99446^**	1.00000

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Universidade de São Paulo, Faculdade de Ciências Farmacêuticas Av. Prof. Lineu Prestes, n. 580, 05508-000 S. Paulo/SP Brasil, Tel.: (55 11) 3091-3824 - São Paulo - SP - Brazil
E-mail: bjps@usp.br

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[TFN1] *: significant at 5% probability level. **: significant at the 1% probability level. ns: not significant