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Medicina Veterinária • Braz. J. Vet. Res. Anim. Sci. 38 (3) • 2001 • https://doi.org/10.1590/S1413-95962001000300010 linkcopy

Open-access Comparison of a commercial enzyme immune assay (ELISA) and serum neutralization techniques for the detection of antibodies against BHV-1 in buffaloes (Bubalus bubalis) sera

AuthorshipSCIMAGO INSTITUTIONS RANKINGS

    The performance of a commercial ELISA (Bovine Rhinotracheitis virus antibody test Kit, Herdcheck®, IDEXX Laboratories Inc., USA) designed to detect the presence of antibody to the Bovine Herpesvirus type 1 (BHV-1) in bovine serum, was evaluated in samples of buffaloes (Bubalus bubalis) serum. Sera of 133 buffaloes without IBR vaccination were performed both by ELISA and standard serum neutralization technique. Sensitivity and specificity of ELISA were 97.14% and 46.03%, respectively. The estimated concordance between two serologic diagnostic methods by kappa index was 0.44 IC (0.29-0.59) using 95% confidence level.

    Infections bovine rhinotracheites; ELISA; Buffaloes; Bubalus bubalis

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    Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária Armando de Salles Oliveira, 05508-270 São Paulo SP Brazil, Tel.: +55 11 3091-7636, Fax: +55 11 3031-3074 / 3091-7672 / 3091-7678 - São Paulo - SP - Brazil
    E-mail: brazvet@edu.usp.br
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