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SDS-PAGE seminal plasma proteins pattern and its correlation with gir breed semen freezability

This study was undertaken to determine whether bovine seminal plasma contained protein markers associated with semen freezability. Seminal plasma was obtained from ten Gir bulls of known fertility and freezability, set into two groups of High (up to 80% of viable sperm after thawing) and Low freezability (less than 50%). SDS-PAGE of seminal plasma samples indicated that one protein (Mr =-20.3;-MW-=-61,800Da) predominated in high-freezability semen. These findings indicate that bull seminal plasma contains freezability-associated proteins which can be used as markers for predicting semen freezability.

Seminal plasma; Cattle; Electrophoresis; Freezing; Semen

Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária Armando de Salles Oliveira, 05508-270 São Paulo SP Brazil, Tel.: +55 11 3091-7636, Fax: +55 11 3031-3074 / 3091-7672 / 3091-7678 - São Paulo - SP - Brazil