It was studied the effects of sucrose and inorganic nitrogen on multiplication of (NH4NO3 and KNO3) "in vitro" culture of Poncirus trifoliata (L.) Raf. Apex buddings from successive multiplications in MS medium added with BAP l.0 mg/l and ANA l.0 mg/l were placed into new MS media where different tested combinations of sucrose (0; 7.5; 15; 30; 45 and 60 g/l) and inorganic nitrogen levels (0 MS; 1/4 MS; 1/2 MS; 1 MS and 2 MS) present in MS medium were tested. The buddings were maintained under the 2,500 lux for 16 daily hours at 27 ± 2°C. After 45 days they were evaluated according characteristics of total number of buds and number of buds higher than 1 cm long. The sucrose and inorganic nitrogen doses affected the rated characteristics. The best responses observed were between 30 to 45 g/l of sucrose associated with double (2 MS) dose of inorganic nitrogen.
tissue culture; Citrus; sucrose; inorganic nitrogen