Isoenzymatic characterization of sugarcane clones and somaclones

Abstracts

Extracts from leaves and roots of three sugarcane varieties and their somaclones: NA56-79 (at seven and nine month old plants), IAC68-12 and IAC68-144 (both at nine month old plants) were obtained. The peroxidase and esterase isoenzyme contents of the extracts were determined to verify the genetic variability of tissue culture somaclones. The peroxidase specific activity was studied in relation to the time and isoenzymatic pattern. The peroxidase and esterase zymograms showed distinct differences among varieties, and the esterase presented less bands but easier to characterize than the peroxidase bands. The root zymograms exhibited great enzymatic variation due to the presence of old roots thus not allowing to characterize the varieties. No differences in peroxidase or esterase zymograms among somaclones of the same variety were noticed. The NA56-79 presented lower specific activity of peroxidase and number of isoenzymes than IAC68-144, suggesting that the decrease of isoenzyme bands is associated to the reduction of enzymatic activity. The specific activity of peroxidase changed with maturity and was higher at nine than at seven month old plants.

sugarcane; isoenzymes; varieties; somaclones

Foram caracterizados os padrões de isoenzimas de peroxidase e esterase de extrato de folha e raiz de cana-de-açúcar de variedade e somaclones de NA56-79 aos 7 meses de idade e de variedades e somaclones de NA56-79, IAC68-12 e IAC68-144 aos 9 meses. Avaliaram-se também as atividades específicas de peroxidase com relação ao tempo e perfil isoenzimático das três variedades e respectivos somaclones. Os zimogramas de peroxidase e esterase de folhas mostraram diferenças distintas entre as três variedades; a esterase apresentou menor número de bandas em relação à peroxidase, porém de mais fácil caracterização. Os zimogramas de raízes, devido à grande variação de atividade enzimática, em conseqüência de raízes velhas, não foram conclusivos quanto à identificação das variedades. Não ocorreram diferenças nos zimogramas de peroxidase ou esterase entre somaclones da mesma variedade. NA56-79 apresentou menor número de isoenzimas e menor atividade especifica de peroxidase em relação à IAC68-144, sugerindo que a diminuição das isoenzimas seja associada ao decréscimo de atividade enzimática. A atividade especifica de peroxidase variou com a maturação, sendo a 9 meses maior do que a 7.

cana-de-açúcar; isoenzimas; variedades; somaclones

HOMAGE

Oswaldo Paulo Forattini

1924-2007

On September 15, 2007, in São Paulo, Brazil lost one of its most prominent scientists in the fields of Public Health, Epidemiology and Medical Entomology, Professor Oswaldo Paulo Forattini, at the age of 83 years.

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*

Com bolsa de pesquisa do CNPq.

Publication Dates

• Publication in this collection
  29 Nov 2007
• Date of issue
  1989

History

• Accepted
  06 Apr 1989
• Received
  23 June 1988