Embryos of early peach and nectarine were cultured on Murashige and Skoog medium containing 3%sacarose,0.6%agar and(in mg/liter) 1 thiamin, 0.5 nicotinic acid, 100 inositol, 250 glycin, 500 glutamin, 250 asparagin, 0.1 giberelic acid, and BAP at concentrations of 0, 5, 10, 15 and 20µM. The frequency of growth was as high as 100% when BAP was included in the medium. The best development of the embryos occurred with BAP rates of 5 and 10µM. At these concentrations of BAP, the mean number of adventitious shoots was 4.5 per embryo. The vitrification ratio was higher at concentrations of 15 and 20µM. The inclusion of BAP, consistently reduced but not fully eliminated lhe rosette and dwarf appearances in vitroplantules.
peach; Prunus persica; nectarine; breeding; embryo culture; vitrification; vitroplantules; rosette; dwarf